Abstract
The Bacillus subtilis sleB gene, which codes for the enzyme homologous to the germination-specific amidase from Bacillus cereus, was cloned and its nucleotide sequence was determined. Sequence analysis showed that it had an open reading frame of 918 bp, coding for a polypeptide of 305 amino acids with a putative signal sequence of 29 residues. Enzyme activity was not found in germination exudate of B. subtilis spores, which differs from the case of B. cereus enzyme. A B. subtilis mutant with an insertionally inactivated sleB gene revealed normal behavior in growth and sporulation. However, the sleB mutant was unable to complete germination mediated by L-alanine.
Full Text
The Full Text of this article is available as a PDF (464.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Foster S. J., Johnstone K. Pulling the trigger: the mechanism of bacterial spore germination. Mol Microbiol. 1990 Jan;4(1):137–141. doi: 10.1111/j.1365-2958.1990.tb02023.x. [DOI] [PubMed] [Google Scholar]
- Foster S. J., Johnstone K. Purification and properties of a germination-specific cortex-lytic enzyme from spores of Bacillus megaterium KM. Biochem J. 1987 Mar 1;242(2):573–579. doi: 10.1042/bj2420573. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Makino S., Ito N., Inoue T., Miyata S., Moriyama R. A spore-lytic enzyme released from Bacillus cereus spores during germination. Microbiology. 1994 Jun;140(Pt 6):1403–1410. doi: 10.1099/00221287-140-6-1403. [DOI] [PubMed] [Google Scholar]
- Miyata S., Moriyama R., Miyahara N., Makino S. A gene (sleC) encoding a spore-cortex-lytic enzyme from Clostridium perfringens S40 spores; cloning, sequence analysis and molecular characterization. Microbiology. 1995 Oct;141(Pt 10):2643–2650. doi: 10.1099/13500872-141-10-2643. [DOI] [PubMed] [Google Scholar]
- Miyata S., Moriyama R., Sugimoto K., Makino S. Purification and partial characterization of a spore cortex-lytic enzyme of Clostridium perfringens S40 spores. Biosci Biotechnol Biochem. 1995 Mar;59(3):514–515. doi: 10.1271/bbb.59.514. [DOI] [PubMed] [Google Scholar]
- Moir A., Smith D. A. The genetics of bacterial spore germination. Annu Rev Microbiol. 1990;44:531–553. doi: 10.1146/annurev.mi.44.100190.002531. [DOI] [PubMed] [Google Scholar]
- Moriyama R., Kudoh S., Miyata S., Nonobe S., Hattori A., Makino S. A germination-specific spore cortex-lytic enzyme from Bacillus cereus spores: cloning and sequencing of the gene and molecular characterization of the enzyme. J Bacteriol. 1996 Sep;178(17):5330–5332. doi: 10.1128/jb.178.17.5330-5332.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sato T., Harada K., Ohta Y., Kobayashi Y. Expression of the Bacillus subtilis spoIVCA gene, which encodes a site-specific recombinase, depends on the spoIIGB product. J Bacteriol. 1994 Feb;176(3):935–937. doi: 10.1128/jb.176.3.935-937.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith H., de Jong A., Bron S., Venema G. Characterization of signal-sequence-coding regions selected from the Bacillus subtilis chromosome. Gene. 1988 Oct 30;70(2):351–361. doi: 10.1016/0378-1119(88)90207-7. [DOI] [PubMed] [Google Scholar]
- Talarico T. L., Dobrogosz W. J. Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri. Appl Environ Microbiol. 1990 Apr;56(4):1195–1197. doi: 10.1128/aem.56.4.1195-1197.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986 Jun 11;14(11):4683–4690. doi: 10.1093/nar/14.11.4683. [DOI] [PMC free article] [PubMed] [Google Scholar]