Abstract
OBJECTIVE: Prevotella bivia is one of the anaerobic bacteria that resides in the flora of the female genital tract. We studied the pathogenicity of P. bivia in a rat pyometra model. METHODS: The experimental animal (rat) model of pyometra was developed to investigate the pathogenicity of P. bivia in a rat pyometra model. RESULTS: In the groups inoculated with aerobes alone, the infection rate was 10% (1/10) in the Staphylococcus aureus- or Staphylococcus agalactiae-inoculated group and 20% (2/10) in the Escherichia coli-inoculated group. Infection was not established in the groups inoculated with anaerobes alone. High infection rates were observed in all the mixed-infection groups. In the S. agalactiae- and Bacteroides fragilis-, S. agalactiae- and P. bivia-, F. coli- and B. fragilis-, and E. coli- and P. bivia-inoculated groups, an infection rate of 100% (10/10) was demonstrated. The efficacy of antibiotics such as flomoxef (FMOX) could be determined using a rat pyometra model. In relation to the alteration of vaginal microbial flora during the menstrual cycle, estrogen increased the growth of P. bivia. CONCLUSION: Mixture of aerobic bacteria and P. bivia increased the pathogenicity of P. bivia. Estrogen would be useful for raising up the inflammatory change of the uterus in experimental models of genital tract infection due to P. bivia.
Full Text
The Full Text of this article is available as a PDF (351.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bartlett J. G., Dezfulian M., Joiner K. Relative efficacy and critical interval of antimicrobial agents in experimental infections involving bacteroides fragilis. Arch Surg. 1983 Feb;118(2):181–184. doi: 10.1001/archsurg.1983.01390020037006. [DOI] [PubMed] [Google Scholar]
- Bartlett J. G., Louie T. J., Gorbach S. L., Onderdonk A. B. Therapeutic efficacy of 29 antimicrobial regimens in experimental intraabdominal sepsis. Rev Infect Dis. 1981 May-Jun;3(3):535–542. doi: 10.1093/clinids/3.3.535. [DOI] [PubMed] [Google Scholar]
- Corbishley C. M. Microbial flora of the vagina and cervix. J Clin Pathol. 1977 Aug;30(8):745–748. doi: 10.1136/jcp.30.8.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris C. A., Morris D. F. Normal vaginal microbiology of women of childbearing age in relation to the use of oral contraceptives and vaginal tampons. J Clin Pathol. 1967 Jul;20(4):636–640. doi: 10.1136/jcp.20.4.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neary M. P., Allen J., Okubadejo O. A., Payne D. J. Preoperative vaginal bacteria and postoperative infections in gynaecological patients. Lancet. 1973 Dec 8;2(7841):1291–1294. doi: 10.1016/s0140-6736(73)92870-5. [DOI] [PubMed] [Google Scholar]
- Ohashi H. [Clinicobacteriological study on microbial flora in the vagina: (1) Variations of vaginal microbial flora according to the menstrual cycle (author's transl)]. Kansenshogaku Zasshi. 1980 Jul;54(7):321–330. doi: 10.11150/kansenshogakuzasshi1970.54.321. [DOI] [PubMed] [Google Scholar]
- Onderdonk A. B., Kasper D. L., Mansheim B. J., Louie T. J., Gorbach S. L., Bartlett J. G. Experimental animal models for anaerobic infections. Rev Infect Dis. 1979 Mar-Apr;1(2):291–301. doi: 10.1093/clinids/1.2.291. [DOI] [PubMed] [Google Scholar]
- Osborne N. G., Wright R. C., Grubin L. Genital bacteriology: a comparative study of premenopausal women with postmenopausal women. Am J Obstet Gynecol. 1979 Sep 15;135(2):195–198. doi: 10.1016/0002-9378(79)90342-9. [DOI] [PubMed] [Google Scholar]
- Sparks R. A., Purrier B. G., Watt P. J., Elstein M. The bacteriology of the cervix and uterus. Br J Obstet Gynaecol. 1977 Sep;84(9):701–704. doi: 10.1111/j.1471-0528.1977.tb12684.x. [DOI] [PubMed] [Google Scholar]