Figure 4.

Polyclonal repopulation with transduced hematopoietic cells. Peripheral blood samples from dogs G272 and G306 were analyzed by LAM-PCR, revealing polyclonal repopulation of both animals. An ethidium-bromide– stained acrylamide gel of LAM-PCR products is shown; L, 50-bp standard. In lanes labeled M, DNA samples were digested with the restriction enzyme MspI, and in lanes labeled B/P, DNA samples were digested with both BspHI and PciI restriction enzymes which have compatible sticky ends. The day after transplantation on which the PB samples were collected is listed below (d117 is day 117 after transplantation).