Table 1.

Transduction and engraftment of canine CD34⁺ cells

Dog	No. of CD34-enriched cells/kg × 106 before culture	Purity of CD34-enriched cells	No. of cells/mL	Amount of virus/mL	Vector titer/mL*	MOI	Infused cells/kg × 106	Transduced CFCs†,%	Days to ANC greater than 500	Days to platelets greater than 50 000
G264	4.9	58	8 × 105	6.8 × 106	5.0 × 107	8.5	2	24	15	NA‡
G272	12.7	78	2.3 × 106	1.9 × 107	1.0 × 108	8.0	5.5	18	10	55
G306	4.8	82	4.7 × 105	4.6 × 106	2.5 × 107	9.8	3.9	11	9	43

CFC indicates colony-forming cell; PB, peripheral blood; NA not applicable.

^*Titer of vector stock preparation prior to addition to transduction culture.

^†Percentage of fluorescence-positive colonies plated immediately after transduction and assessed on day 14 by fluorescence microscopy.

^‡Animal died 17 days after transplantation because of an intussusception.