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. 1996 Nov;178(21):6366–6368. doi: 10.1128/jb.178.21.6366-6368.1996

Role of the two-component signal transduction and the phosphoenolpyruvate: carbohydrate phosphotransferase systems in competence development of Haemophilus influenzae Rd.

M L Gwinn 1, D Yi 1, H O Smith 1, J F Tomb 1
PMCID: PMC178514  PMID: 8892843

Abstract

Haemophilus influenzae Rd becomes competent for transformation by nutritional downshift or transient anaerobic growth through a process that requires cyclic AMP receptor protein and adenylate cyclase. Insertion mutations in crr or ptsI of the phosphoenolpyruvate:carbohydrate phosphotransferase system lowered transformation frequencies, and the effect was reversed by the addition of cyclic AMP. However, insertions into H. influenzae homologs of two-component signal transduction genes had no effect on competence.

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Selected References

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  1. Barcak G. J., Chandler M. S., Redfield R. J., Tomb J. F. Genetic systems in Haemophilus influenzae. Methods Enzymol. 1991;204:321–342. doi: 10.1016/0076-6879(91)04016-h. [DOI] [PubMed] [Google Scholar]
  2. Botsford J. L., Harman J. G. Cyclic AMP in prokaryotes. Microbiol Rev. 1992 Mar;56(1):100–122. doi: 10.1128/mr.56.1.100-122.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chandler M. S., Smith R. A. Characterization of the Haemophilus influenzae topA locus: DNA topoisomerase I is required for genetic competence. Gene. 1996 Feb 22;169(1):25–31. doi: 10.1016/0378-1119(95)00777-6. [DOI] [PubMed] [Google Scholar]
  4. Chandler M. S. The gene encoding cAMP receptor protein is required for competence development in Haemophilus influenzae Rd. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1626–1630. doi: 10.1073/pnas.89.5.1626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Danese P. N., Snyder W. B., Cosma C. L., Davis L. J., Silhavy T. J. The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP. Genes Dev. 1995 Feb 15;9(4):387–398. doi: 10.1101/gad.9.4.387. [DOI] [PubMed] [Google Scholar]
  6. Dorocicz I. R., Williams P. M., Redfield R. J. The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence. J Bacteriol. 1993 Nov;175(22):7142–7149. doi: 10.1128/jb.175.22.7142-7149.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fleischmann R. D., Adams M. D., White O., Clayton R. A., Kirkness E. F., Kerlavage A. R., Bult C. J., Tomb J. F., Dougherty B. A., Merrick J. M. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. 1995 Jul 28;269(5223):496–512. doi: 10.1126/science.7542800. [DOI] [PubMed] [Google Scholar]
  8. GOODGAL S. H., HERRIOTT R. M. Studies on transformations of Hemophilus influenzae. I. Competence. J Gen Physiol. 1961 Jul;44:1201–1227. doi: 10.1085/jgp.44.6.1201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herriott R. M., Meyer E. M., Vogt M. Defined nongrowth media for stage II development of competence in Haemophilus influenzae. J Bacteriol. 1970 Feb;101(2):517–524. doi: 10.1128/jb.101.2.517-524.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Macfadyen L. P., Dorocicz I. R., Reizer J., Saier M. H., Jr, Redfield R. J. Regulation of competence development and sugar utilization in Haemophilus influenzae Rd by a phosphoenolpyruvate:fructose phosphotransferase system. Mol Microbiol. 1996 Sep;21(5):941–952. doi: 10.1046/j.1365-2958.1996.441420.x. [DOI] [PubMed] [Google Scholar]
  11. Nagasawa S., Ishige K., Mizuno T. Novel members of the two-component signal transduction genes in Escherichia coli. J Biochem. 1993 Sep;114(3):350–357. doi: 10.1093/oxfordjournals.jbchem.a124180. [DOI] [PubMed] [Google Scholar]
  12. Postma P. W., Lengeler J. W., Jacobson G. R. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev. 1993 Sep;57(3):543–594. doi: 10.1128/mr.57.3.543-594.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rabin R. S., Stewart V. Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12. J Bacteriol. 1993 Jun;175(11):3259–3268. doi: 10.1128/jb.175.11.3259-3268.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Redfield R. J. sxy-1, a Haemophilus influenzae mutation causing greatly enhanced spontaneous competence. J Bacteriol. 1991 Sep;173(18):5612–5618. doi: 10.1128/jb.173.18.5612-5618.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spiro S., Guest J. R. Adaptive responses to oxygen limitation in Escherichia coli. Trends Biochem Sci. 1991 Aug;16(8):310–314. doi: 10.1016/0968-0004(91)90125-f. [DOI] [PubMed] [Google Scholar]
  16. Stock J. B., Stock A. M., Mottonen J. M. Signal transduction in bacteria. Nature. 1990 Mar 29;344(6265):395–400. doi: 10.1038/344395a0. [DOI] [PubMed] [Google Scholar]
  17. Wanner B. L. Gene regulation by phosphate in enteric bacteria. J Cell Biochem. 1993 Jan;51(1):47–54. doi: 10.1002/jcb.240510110. [DOI] [PubMed] [Google Scholar]
  18. Williams P. M., Bannister L. A., Redfield R. J. The Haemophilus influenzae sxy-1 mutation is in a newly identified gene essential for competence. J Bacteriol. 1994 Nov;176(22):6789–6794. doi: 10.1128/jb.176.22.6789-6794.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wise E. M., Jr, Alexander S. P., Powers M. Adenosine 3':5'-cyclic monophosphate as a regulator of bacterial transformation. Proc Natl Acad Sci U S A. 1973 Feb;70(2):471–474. doi: 10.1073/pnas.70.2.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zulty J. J., Barcak G. J. Identification of a DNA transformation gene required for com101A+ expression and supertransformer phenotype in Haemophilus influenzae. Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3616–3620. doi: 10.1073/pnas.92.8.3616. [DOI] [PMC free article] [PubMed] [Google Scholar]

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