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. 1996 Nov;178(21):6403–6406. doi: 10.1128/jb.178.21.6403-6406.1996

Genetic and chemical characterization of a mutant that disrupts synthesis of the lipopolysaccharide core tetrasaccharide in Rhizobium leguminosarum.

D Allaway 1, B Jeyaretnam 1, R W Carlson 1, P S Poole 1
PMCID: PMC178523  PMID: 8892852

Abstract

A 2-kb region that complements the Tn5-derived lipopolysaccharide (LPS) rough mutant Rhizobium leguminosarum RU301 was sequenced. Two open reading frames (ORFs) were identified. The first ORF (lpcA) is homologous to a family of bacterial sugar transferases involved in LPS core tetrasaccharide biosynthesis. ORF2 (lpcB), in which Tn5 transposed, has no significant homology to any DNA in the GenBank-EMBL databases. Chemical characterization of LPS produced by strain RU301 demonstrated that the 3-deoxy-D-manno-2-octulosonic acid (Kdo) residue which normally attaches the core tetrasaccharide to the O chain was missing, suggesting that IpcB may encode a CMP-Kdo:LPS Kdo transferase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhat U. R., Krishnaiah B. S., Carlson R. W. Re-examination of the structures of the lipopolysaccharide core oligosaccharides from Rhizobium leguminosarum biovar phaseoli. Carbohydr Res. 1991 Nov 11;220:219–227. doi: 10.1016/0008-6215(91)80020-n. [DOI] [PubMed] [Google Scholar]
  2. Carlson R. W., Reuhs B., Chen T. B., Bhat U. R., Noel K. D. Lipopolysaccharide core structures in Rhizobium etli and mutants deficient in O-antigen. J Biol Chem. 1995 May 19;270(20):11783–11788. doi: 10.1074/jbc.270.20.11783. [DOI] [PubMed] [Google Scholar]
  3. Cava J. R., Elias P. M., Turowski D. A., Noel K. D. Rhizobium leguminosarum CFN42 genetic regions encoding lipopolysaccharide structures essential for complete nodule development on bean plants. J Bacteriol. 1989 Jan;171(1):8–15. doi: 10.1128/jb.171.1.8-15.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diebold R., Noel K. D. Rhizobium leguminosarum exopolysaccharide mutants: biochemical and genetic analyses and symbiotic behavior on three hosts. J Bacteriol. 1989 Sep;171(9):4821–4830. doi: 10.1128/jb.171.9.4821-4830.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gotschlich E. C. Genetic locus for the biosynthesis of the variable portion of Neisseria gonorrhoeae lipooligosaccharide. J Exp Med. 1994 Dec 1;180(6):2181–2190. doi: 10.1084/jem.180.6.2181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hollingsworth R. I., Carlson R. W., Garcia F., Gage D. A. A new core tetrasaccharide component from the lipopolysaccharide of Rhizobium trifolii ANU 843. J Biol Chem. 1989 Jun 5;264(16):9294–9299. [PubMed] [Google Scholar]
  7. Mavridou A., Barny M. A., Poole P., Plaskitt K., Davies A. E., Johnston A. W., Downie J. A. Rhizobium leguminosarum nodulation gene (nod) expression is lowered by an allele-specific mutation in the dicarboxylate transport gene dctB. Microbiology. 1995 Jan;141(Pt 1):103–111. doi: 10.1099/00221287-141-1-103. [DOI] [PubMed] [Google Scholar]
  8. Noel K. D., Vandenbosch K. A., Kulpaca B. Mutations in Rhizobium phaseoli that lead to arrested development of infection threads. J Bacteriol. 1986 Dec;168(3):1392–1401. doi: 10.1128/jb.168.3.1392-1401.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Poole P. S., Schofield N. A., Reid C. J., Drew E. M., Walshaw D. L. Identification of chromosomal genes located downstream of dctD that affect the requirement for calcium and the lipopolysaccharide layer of Rhizobium leguminosarum. Microbiology. 1994 Oct;140(Pt 10):2797–2809. doi: 10.1099/00221287-140-10-2797. [DOI] [PubMed] [Google Scholar]
  10. Pradel E., Parker C. T., Schnaitman C. A. Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core. J Bacteriol. 1992 Jul;174(14):4736–4745. doi: 10.1128/jb.174.14.4736-4745.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Priefer U. B. Genes involved in lipopolysaccharide production and symbiosis are clustered on the chromosome of Rhizobium leguminosarum biovar viciae VF39. J Bacteriol. 1989 Nov;171(11):6161–6168. doi: 10.1128/jb.171.11.6161-6168.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zhang Y., Hollingsworth R. I., Priefer U. B. Characterization of structural defects in the lipopolysaccharides of symbiotically impaired Rhizobium leguminosarum biovar viciae VF-39 mutants. Carbohydr Res. 1992 Jul 2;231:261–271. doi: 10.1016/0008-6215(92)84024-m. [DOI] [PubMed] [Google Scholar]

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