NEUROSCIENCE. For the article “Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses,” by Anita Scheuber, Rachel Rudge, Lydia Danglot, Graca Raposo, Thomas Binz, Jean-Christophe Poncer, and Thierry Galli, which appeared in issue 44, October 31, 2006, of Proc Natl Acad Sci USA (103:16562–16567; first published October 20, 2006; 10.1073/pnas.0603511103), the authors note that Fig. 3g was labeled incorrectly. The corrected figure and its legend appear below.
Fig. 3.
Ca-independent quantal release at excitatory synapses on CA3 cells in control and mocha cultured slices. (a) Representative traces of mEPSCs recorded in CA3 pyramidal cells from control (+/−) and mocha (−/−) cultures, treated or not with TeNT. (b) Averaged mEPSCs (≈100) detected from the above recordings. Black traces, control; blue traces, after TeNT treatment. No difference in their rate of either onset or decay was apparent. (c) (Left) Average amplitude of mEPSCs recorded in all four conditions. No significant difference was observed (n = 7, 9, 11, and 8 cells, respectively; P > 0.05). (Right) Cumulative amplitude histograms from the same four data sets. The distributions were not significantly different (Kolmogorov–Smirnov test, P > 0.05). (d) Mean frequencies of mEPSCs were significantly different between control and TeNT-treated cultures in both control and mocha cultures (P < 0.005 and P < 0.05, respectively). mEPSC frequency was also different in control vs. mocha culture in the absence of TeNT (P < 0.05). (e) mocha culture slices treated with TeNT for 72 h were fixed and labeled with antibodies against SNAP25 (red), TeNT (green), and DAPI (blue). The whole surface of the explant can be visualized by either DAPI (nucleus) or SNAP25 (neuronal plasma membrane). Note that TeNT staining is uniformly distributed, confirming the extended penetration of the toxin. (Scale bar, 200 μm.) (f) Culture slices used in electrophysiological recordings were lysed and analyzed by Western blotting with antibodies against AP-3δ, Syb2, and actin (as a loading control). A 72-h treatment with TeNT resulted in efficient cleavage of Syb2, although quantification of the remaining Syb2 revealed a 2-fold increase in TeNT-resistant Syb2 in mocha slices [mean ± SEM; control 17.83 ± 2.35, n = 6; mocha 37.58 ± 6.31, n = 6; P < 0.015 (Mann–Whitney rank sum test)]. (g) mocha-cultured slices treated with or without TeNT for 72 h were fixed and labeled with antibodies against Syp (green), Syb2 (red), and DAPI (blue). Note that the remaining Syb2 labeling after TeNT treatment is very faint. The rare remaining Syb2 puncta are mainly synaptic. (Scale bar, 50 μm.)