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. 1996 Dec;178(24):7304–7307. doi: 10.1128/jb.178.24.7304-7307.1996

Inhibition of RecA-mediated cleavage in covalent dimers of UmuD.

M H Lee 1, A Guzzo 1, G C Walker 1
PMCID: PMC178647  PMID: 8955416

Abstract

Disulfide-cross-linked UmuD2 derivatives were cleaved poorly upon incubation with activated RecA. Reducing the disulfide bonds prior to incubating the derivatives with RecA dramatically increased their extent of cleavage. These observations suggest that the UmuD monomer is a better substrate for the RecA-mediated cleavage reaction than the dimer.

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Selected References

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