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. 1997 Jan;179(1):272–275. doi: 10.1128/jb.179.1.272-275.1997

Effects of inactivation or overexpression of the sspF gene on properties of Bacillus subtilis spores.

C A Loshon 1, P Kraus 1, B Setlow 1, P Setlow 1
PMCID: PMC178689  PMID: 8982008

Abstract

Inactivation of the Bacillus subtilis sspF gene had no effect on sporulation, spore resistance, or germination in a wild-type strain or one lacking DNA protective alpha/beta-type small, acid-soluble proteins (SASP). Overexpression of SspF in wild-type spores or in spores lacking major alpha/beta-type SASP (alpha- beta- spores) had no effect on sporulation but slowed spore outgrowth and restored a small amount of UV and heat resistance to alpha- beta- spores. In vitro analyses showed that SspF is a DNA binding protein and is cleaved by the SASP-specific protease (GPR) at a site similar to that cleaved in alpha/beta-type SASP. SspF was also degraded during spore germination and outgrowth, and this degradation was initiated by GPR.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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