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. 1997 Feb;179(3):949–951. doi: 10.1128/jb.179.3.949-951.1997

Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression.

C Haussmann 1, F Rohdich 1, F Lottspeich 1, S Eberhardt 1, J Scheuring 1, S Mackamul 1, A Bacher 1
PMCID: PMC178780  PMID: 9006053

Abstract

The enzyme catalyzing the epimerization at position 2' of dihydroneopterin triphosphate was purified by a factor of about 10,000 from cell extract of Escherichia coli. The cognate gene was cloned, sequenced, expressed, and mapped to kb 2427 on the E. coli chromosome.

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Selected References

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  1. Heine M. C., Brown G. M. Enzymatic epimerization of D-erythro-dihydroneopterin triphosphate to L-threo-dihydroneopterin triphosphate. Biochim Biophys Acta. 1975 Dec 5;411(2):236–249. doi: 10.1016/0304-4165(75)90304-9. [DOI] [PubMed] [Google Scholar]
  2. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  3. Mathis J. B., Brown G. M. Dihydroneopterin aldolase from Escherichia coli. Methods Enzymol. 1980;66:556–560. doi: 10.1016/0076-6879(80)66506-9. [DOI] [PubMed] [Google Scholar]
  4. Mathis J. B., Brown G. M. The biosynthesis of folic acid. XI. Purification and properties of dihydroneopterin aldolase. J Biol Chem. 1970 Jun 10;245(11):3015–3025. [PubMed] [Google Scholar]
  5. Volpe F., Ballantine S. P., Delves C. J. Two domains with amino-acid sequence similarity are required for dihydroneopterin aldolase function in the multifunctional folic acid synthesis Fas protein of Pneumocystis carinii. Gene. 1995 Jul 4;160(1):41–46. doi: 10.1016/0378-1119(95)00203-i. [DOI] [PubMed] [Google Scholar]

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