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. 1997 Mar;179(5):1805–1808. doi: 10.1128/jb.179.5.1805-1808.1997

Primary structure and properties of the Na+/glucose symporter (Sg1S) of Vibrio parahaemolyticus.

R I Sarker 1, W Ogawa 1, T Shimamoto 1, T Shimamoto 1, T Tsuchiya 1
PMCID: PMC178897  PMID: 9045844

Abstract

Previously, we cloned and sequenced a DNA fragment from Vibrio parahaemolyticus and found four open reading frames (ORFs). Here, we clearly demonstrate that one of the ORFs, ORF1, is the gene (sglS) encoding a Na+/glucose symporter (SglS). We characterize the Na+/glucose symporter produced in Escherichia coli mutant (JM1100) cells which lack original glucose transport activity and galactose transport activity. We also show that phlorizin, a potent inhibitor of the SGLT1 Na+/glucose symporter of animal cells, inhibited glucose transport, but not galactose transport, via the SglS system.

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Selected References

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