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. 1997 Mar;179(5):1819–1823. doi: 10.1128/jb.179.5.1819-1823.1997

In vivo evidence that acyl coenzyme A regulates DNA binding by the Escherichia coli FadR global transcription factor.

J E Cronan Jr 1
PMCID: PMC178900  PMID: 9045847

Abstract

In vitro experiments point to fatty acyl coenzymes A (acyl-CoAs) rather than unesterified fatty acids as the small-molecule ligands regulating DNA binding by the FadR protein of Escherichia coli. To provide an in vivo test of this specificity, unesterified fatty acids were generated within the cellular cytosol. These fatty acids were found to be efficient modulators of FadR action only when the acids could be converted to acyl-CoAs.

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Selected References

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