Abstract
Loss of 3, 7, or 10 of the amino-terminal 15 residues removed upon autoactivation of the zymogen of the germination protease (GPR), which initiates protein degradation during germination of spores of Bacillus species, did not result in significant changes in (i) the lack of enzymatic activity of the zymogen, (ii) the rate of zymogen autoactivation, or (iii) the unreactivity of the zymogen's single SH group. Removal of 13 amino-terminal residues resulted in a partially active enzyme whose SH group was as reactive as the fully active enzyme. These findings suggest that at least a part of the propeptide blocks access to the enzyme's active site. However, the free propeptide did not inhibit the enzyme.
Full Text
The Full Text of this article is available as a PDF (322.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Illades-Aguiar B., Setlow P. Autoprocessing of the protease that degrades small, acid-soluble proteins of spores of Bacillus species is triggered by low pH, dehydration, and dipicolinic acid. J Bacteriol. 1994 Nov;176(22):7032–7037. doi: 10.1128/jb.176.22.7032-7037.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Illades-Aguiar B., Setlow P. Studies of the processing of the protease which initiates degradation of small, acid-soluble proteins during germination of spores of Bacillus species. J Bacteriol. 1994 May;176(10):2788–2795. doi: 10.1128/jb.176.10.2788-2795.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Illades-Aguiar B., Setlow P. The zymogen of the protease that degrades small, acid-soluble proteins of spores of Bacillus species can rapidly autoprocess to the active enzyme in vitro. J Bacteriol. 1994 Sep;176(17):5571–5573. doi: 10.1128/jb.176.17.5571-5573.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loshon C. A., Setlow P. Bacillus megaterium spore protease: purification, radioimmunoassay, and analysis of antigen level and localization during growth, sporulation, and spore germination. J Bacteriol. 1982 Apr;150(1):303–311. doi: 10.1128/jb.150.1.303-311.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loshon C. A., Swerdlow B. M., Setlow P. Bacillus megaterium spore protease. Synthesis and processing of precursor forms during sporulation and germination. J Biol Chem. 1982 Sep 25;257(18):10838–10845. [PubMed] [Google Scholar]
- Markaryan A., Lee J. D., Sirakova T. D., Kolattukudy P. E. Specific inhibition of mature fungal serine proteinases and metalloproteinases by their propeptides. J Bacteriol. 1996 Apr;178(8):2211–2215. doi: 10.1128/jb.178.8.2211-2215.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanchez-Salas J. L., Setlow P. Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores. J Bacteriol. 1993 May;175(9):2568–2577. doi: 10.1128/jb.175.9.2568-2577.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sarkar G., Sommer S. S. The "megaprimer" method of site-directed mutagenesis. Biotechniques. 1990 Apr;8(4):404–407. [PubMed] [Google Scholar]
- Setlow P. Small, acid-soluble spore proteins of Bacillus species: structure, synthesis, genetics, function, and degradation. Annu Rev Microbiol. 1988;42:319–338. doi: 10.1146/annurev.mi.42.100188.001535. [DOI] [PubMed] [Google Scholar]
- Sussman M. D., Setlow P. Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination. J Bacteriol. 1991 Jan;173(1):291–300. doi: 10.1128/jb.173.1.291-300.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]