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. 1997 Mar;179(6):2068–2072. doi: 10.1128/jb.179.6.2068-2072.1997

Isolation and characterization of Rhizobium etli mutants altered in degradation of asparagine.

A Huerta-Zepeda 1, L Ortuño 1, G Du Pont 1, S Durán 1, A Lloret 1, H Merchant-Larios 1, J Calderón 1
PMCID: PMC178935  PMID: 9068657

Abstract

Rhizobium etli mutants unable to grow on asparagine as the nitrogen and carbon source were isolated. Two kinds of mutants were obtained: AHZ1, with very low levels of aspartase activity, and AHZ7, with low levels of asparaginase and very low levels of aspartase compared to the wild-type strain. R. etli had two asparaginases differentiated by their thermostabilities, electrophoretic mobilities, and modes of regulation. The AHZ mutants nodulated as did the wild-type strain and had nitrogenase levels similar to that of the wild-type strain.

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Selected References

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