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. 1997 Apr;179(7):2449–2451. doi: 10.1128/jb.179.7.2449-2451.1997

A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.

W Jäger 1, J Kalinowski 1, A Pühler 1
PMCID: PMC178988  PMID: 9079937

Abstract

A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters.

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Selected References

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