Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1997 May;179(9):2840–2844. doi: 10.1128/jb.179.9.2840-2844.1997

The A protein of the filamentous bacteriophage Cf of Xanthomonas campestris pv. citri.

M K Yang 1, Y C Yang 1
PMCID: PMC179043  PMID: 9139897

Abstract

Filamentous bacteriophages have very strict host specificities. Experiments were performed to investigate whether the A protein of the filamentous phage Cf, which infects Xanthomonas campestris pv. citri but not X. campestris pv. oryzae, is involved in determining Cf's host specificity. The gene encoding the A protein of Cf was cloned and expressed in X. campestris pv. citri. The genomic DNA of another filamentous bacteriophage, Xf, which infects X. campestris pv. oryzae but not X. campestris pv. citri, was then introduced by electroporation into X. campestris pv. citri that had expressed the A protein of Cf. The progeny phages thus produced were able to infect both X. campestris pv. oryzae and X. campestris pv. citri, indicating that the A protein of Cf was incorporated into the viral particles of Xf and conferred upon Xf the ability to infect the host of Cf. Inactivation of the A protein gene abolished the infectivity of Cf. The results of this study indicate that the A protein of Cf is responsible for controlling the host specificity of Cf.

Full Text

The Full Text of this article is available as a PDF (383.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bross P., Bussmann K., Keppner W., Rasched I. Functional analysis of the adsorption protein of two filamentous phages with different host specificities. J Gen Microbiol. 1988 Feb;134(2):461–471. doi: 10.1099/00221287-134-2-461. [DOI] [PubMed] [Google Scholar]
  3. Caro L. G., Schnös M. The attachment of the male-specific bacteriophage F1 to sensitive strains of Escherichia coli. Proc Natl Acad Sci U S A. 1966 Jul;56(1):126–132. doi: 10.1073/pnas.56.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Denhardt D. T. The single-stranded DNA phages. CRC Crit Rev Microbiol. 1975 Dec;4(2):161–223. doi: 10.3109/10408417509111575. [DOI] [PubMed] [Google Scholar]
  5. Endemann H., Gailus V., Rasched I. Interchangeability of the adsorption proteins of bacteriophages Ff and IKe. J Virol. 1993 Jun;67(6):3332–3337. doi: 10.1128/jvi.67.6.3332-3337.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldsmith M. E., Konigsberg W. H. Adsorption protein of the bacteriophage fd: isolation, molecular properties, and location in the virus. Biochemistry. 1977 Jun 14;16(12):2686–2694. doi: 10.1021/bi00631a016. [DOI] [PubMed] [Google Scholar]
  7. Grant R. A., Lin T. C., Konigsberg W., Webster R. E. Structure of the filamentous bacteriophage fl. Location of the A, C, and D minor coat proteins. J Biol Chem. 1981 Jan 10;256(1):539–546. [PubMed] [Google Scholar]
  8. Gray C. W., Brown R. S., Marvin D. A. Adsorption complex of filamentous fd virus. J Mol Biol. 1981 Mar 15;146(4):621–627. doi: 10.1016/0022-2836(81)90050-4. [DOI] [PubMed] [Google Scholar]
  9. Henry T. J., Pratt D. The proteins of bacteriophage M13. Proc Natl Acad Sci U S A. 1969 Mar;62(3):800–807. doi: 10.1073/pnas.62.3.800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kuo T. T., Huang T. C., Chow T. Y. A filamentous bacteriophage from Xanthomonas oryzae. Virology. 1969 Nov;39(3):548–555. doi: 10.1016/0042-6822(69)90102-0. [DOI] [PubMed] [Google Scholar]
  11. Kuo T. T., Tan M. S., Su M. T., Yang M. K. Complete nucleotide sequence of filamentous phage Cf1c from Xanthomonas campestris pv. citri. Nucleic Acids Res. 1991 May 11;19(9):2498–2498. doi: 10.1093/nar/19.9.2498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lin T. C., Bendet I. J. The A protein of bacteriophage fd: its interference with viral infection. Biochem Biophys Res Commun. 1976 Sep 7;72(1):369–372. doi: 10.1016/0006-291x(76)91003-2. [DOI] [PubMed] [Google Scholar]
  13. Marvin D. A., Hohn B. Filamentous bacterial viruses. Bacteriol Rev. 1969 Jun;33(2):172–209. doi: 10.1128/br.33.2.172-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rasched I., Oberer E. Ff coliphages: structural and functional relationships. Microbiol Rev. 1986 Dec;50(4):401–427. doi: 10.1128/mr.50.4.401-427.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Russel M. Filamentous phage assembly. Mol Microbiol. 1991 Jul;5(7):1607–1613. doi: 10.1111/j.1365-2958.1991.tb01907.x. [DOI] [PubMed] [Google Scholar]
  16. Russel M. Interchangeability of related proteins and autonomy of function. The morphogenetic proteins of filamentous phage f1 and IKe cannot replace one another. J Mol Biol. 1992 Sep 20;227(2):453–462. doi: 10.1016/0022-2836(92)90900-5. [DOI] [PubMed] [Google Scholar]
  17. Stengele I., Bross P., Garcés X., Giray J., Rasched I. Dissection of functional domains in phage fd adsorption protein. Discrimination between attachment and penetration sites. J Mol Biol. 1990 Mar 5;212(1):143–149. doi: 10.1016/0022-2836(90)90311-9. [DOI] [PubMed] [Google Scholar]
  18. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES