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. 1997 May;179(9):3039–3042. doi: 10.1128/jb.179.9.3039-3042.1997

Cloning and characterization of cspL and cspP, two cold-inducible genes from Lactobacillus plantarum.

B Mayo 1, S Derzelle 1, M Fernández 1, C Léonard 1, T Ferain 1, P Hols 1, J E Suárez 1, J Delcour 1
PMCID: PMC179071  PMID: 9139925

Abstract

Two cold shock genes, cspL and cspP, have been cloned from two Lactobacillus plantarum strains. These genes, which are nonallelic, were present in all strains tested. The genes encode 66-amino-acid polypeptides related to each other and to the cold shock Csp family. Transcription of cspP rendered a single mRNA, while two cspL mRNAs were found with common 5' ends. The amounts of these transcripts increased moderately upon exposure of the cultures to cold.

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Selected References

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  1. Fang L., Jiang W., Bae W., Inouye M. Promoter-independent cold-shock induction of cspA and its derepression at 37 degrees C by mRNA stabilization. Mol Microbiol. 1997 Jan;23(2):355–364. doi: 10.1046/j.1365-2958.1997.2351592.x. [DOI] [PubMed] [Google Scholar]
  2. Goldstein J., Pollitt N. S., Inouye M. Major cold shock protein of Escherichia coli. Proc Natl Acad Sci U S A. 1990 Jan;87(1):283–287. doi: 10.1073/pnas.87.1.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Graumann P., Marahiel M. A. The major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides. FEBS Lett. 1994 Jan 31;338(2):157–160. doi: 10.1016/0014-5793(94)80355-2. [DOI] [PubMed] [Google Scholar]
  4. Graumann P., Schröder K., Schmid R., Marahiel M. A. Cold shock stress-induced proteins in Bacillus subtilis. J Bacteriol. 1996 Aug;178(15):4611–4619. doi: 10.1128/jb.178.15.4611-4619.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hebraud M., Dubois E., Potier P., Labadie J. Effect of growth temperatures on the protein levels in a psychrotrophic bacterium, Pseudomonas fragi. J Bacteriol. 1994 Jul;176(13):4017–4024. doi: 10.1128/jb.176.13.4017-4024.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jones P. G., Inouye M. The cold-shock response--a hot topic. Mol Microbiol. 1994 Mar;11(5):811–818. doi: 10.1111/j.1365-2958.1994.tb00359.x. [DOI] [PubMed] [Google Scholar]
  7. Jones P. G., Krah R., Tafuri S. R., Wolffe A. P. DNA gyrase, CS7.4, and the cold shock response in Escherichia coli. J Bacteriol. 1992 Sep;174(18):5798–5802. doi: 10.1128/jb.174.18.5798-5802.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones P. G., VanBogelen R. A., Neidhardt F. C. Induction of proteins in response to low temperature in Escherichia coli. J Bacteriol. 1987 May;169(5):2092–2095. doi: 10.1128/jb.169.5.2092-2095.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. La Teana A., Brandi A., Falconi M., Spurio R., Pon C. L., Gualerzi C. O. Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10907–10911. doi: 10.1073/pnas.88.23.10907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee S. J., Xie A., Jiang W., Etchegaray J. P., Jones P. G., Inouye M. Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli, whose members show a high sequence similarity with the eukaryotic Y-box binding proteins. Mol Microbiol. 1994 Mar;11(5):833–839. doi: 10.1111/j.1365-2958.1994.tb00361.x. [DOI] [PubMed] [Google Scholar]
  11. Mayr B., Kaplan T., Lechner S., Scherer S. Identification and purification of a family of dimeric major cold shock protein homologs from the psychrotrophic Bacillus cereus WSBC 10201. J Bacteriol. 1996 May;178(10):2916–2925. doi: 10.1128/jb.178.10.2916-2925.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakashima K., Kanamaru K., Mizuno T., Horikoshi K. A novel member of the cspA family of genes that is induced by cold shock in Escherichia coli. J Bacteriol. 1996 May;178(10):2994–2997. doi: 10.1128/jb.178.10.2994-2997.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pouwels P. H., Leer R. J. Genetics of lactobacilli: plasmids and gene expression. Antonie Van Leeuwenhoek. 1993;64(2):85–107. doi: 10.1007/BF00873020. [DOI] [PubMed] [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sawers G., Böck A. Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression. J Bacteriol. 1989 May;171(5):2485–2498. doi: 10.1128/jb.171.5.2485-2498.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tanabe H., Goldstein J., Yang M., Inouye M. Identification of the promoter region of the Escherichia coli major cold shock gene, cspA. J Bacteriol. 1992 Jun;174(12):3867–3873. doi: 10.1128/jb.174.12.3867-3873.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Willimsky G., Bang H., Fischer G., Marahiel M. A. Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures. J Bacteriol. 1992 Oct;174(20):6326–6335. doi: 10.1128/jb.174.20.6326-6335.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wolffe A. P., Tafuri S., Ranjan M., Familari M. The Y-box factors: a family of nucleic acid binding proteins conserved from Escherichia coli to man. New Biol. 1992 Apr;4(4):290–298. [PubMed] [Google Scholar]
  19. Yamanaka K., Mitani T., Ogura T., Niki H., Hiraga S. Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli. Mol Microbiol. 1994 Jul;13(2):301–312. doi: 10.1111/j.1365-2958.1994.tb00424.x. [DOI] [PubMed] [Google Scholar]

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