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. 1997 May;179(10):3365–3367. doi: 10.1128/jb.179.10.3365-3367.1997

Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coli.

O I Sanders 1, C Rensing 1, M Kuroda 1, B Mitra 1, B P Rosen 1
PMCID: PMC179121  PMID: 9150238

Abstract

In a search for genes responsible for the accumulation of antimonite in Escherichia coli, TnphoA was used to create a pool of random insertional mutants, from which one antimonite-resistant mutant was isolated. Sequence analysis showed that the TnphoA insertion was located in the glpF gene, coding for the glycerol facilitator GlpF. The mutant was shown to be defective in polyol transport by GlpF. These results suggest that in solution Sb(III) is recognized as a polyol by the glycerol facilitator.

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Selected References

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  1. Carlin A., Shi W., Dey S., Rosen B. P. The ars operon of Escherichia coli confers arsenical and antimonial resistance. J Bacteriol. 1995 Feb;177(4):981–986. doi: 10.1128/jb.177.4.981-986.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chen C. M., Misra T. K., Silver S., Rosen B. P. Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon. J Biol Chem. 1986 Nov 15;261(32):15030–15038. [PubMed] [Google Scholar]
  3. Heller K. B., Lin E. C., Wilson T. H. Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli. J Bacteriol. 1980 Oct;144(1):274–278. doi: 10.1128/jb.144.1.274-278.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
  5. Kuroda M., Dey S., Sanders O. I., Rosen B. P. Alternate energy coupling of ArsB, the membrane subunit of the Ars anion-translocating ATPase. J Biol Chem. 1997 Jan 3;272(1):326–331. doi: 10.1074/jbc.272.1.326. [DOI] [PubMed] [Google Scholar]
  6. Manoil C., Beckwith J. TnphoA: a transposon probe for protein export signals. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129–8133. doi: 10.1073/pnas.82.23.8129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mobley H. L., Rosen B. P. Energetics of plasmid-mediated arsenate resistance in Escherichia coli. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6119–6122. doi: 10.1073/pnas.79.20.6119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Plunkett G., 3rd, Burland V., Daniels D. L., Blattner F. R. Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 1993 Jul 25;21(15):3391–3398. doi: 10.1093/nar/21.15.3391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Silver S., Budd K., Leahy K. M., Shaw W. V., Hammond D., Novick R. P., Willsky G. R., Malamy M. H., Rosenberg H. Inducible plasmid-determined resistance to arsenate, arsenite, and antimony (III) in escherichia coli and Staphylococcus aureus. J Bacteriol. 1981 Jun;146(3):983–996. doi: 10.1128/jb.146.3.983-996.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sweet G., Gandor C., Voegele R., Wittekindt N., Beuerle J., Truniger V., Lin E. C., Boos W. Glycerol facilitator of Escherichia coli: cloning of glpF and identification of the glpF product. J Bacteriol. 1990 Jan;172(1):424–430. doi: 10.1128/jb.172.1.424-430.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weissenborn D. L., Wittekindt N., Larson T. J. Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12. J Biol Chem. 1992 Mar 25;267(9):6122–6131. [PubMed] [Google Scholar]
  13. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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