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. 1997 May;179(10):3371–3373. doi: 10.1128/jb.179.10.3371-3373.1997

The Bacillus subtilis ureABC operon.

H Cruz-Ramos 1, P Glaser 1, L V Wray Jr 1, S H Fisher 1
PMCID: PMC179123  PMID: 9150240

Abstract

The Bacillus subtilis ureABC operon encodes homologs of the three subunits of urease enzymes of the family Enterobacteriaceae. Disruption of ureC prevented utilization of urea as a nitrogen source and resulted in a partial growth defect in minimal medium containing limiting amounts of arginine or allantoin as the sole nitrogen source.

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Selected References

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  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Atkinson M. R., Fisher S. H. Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis. J Bacteriol. 1991 Jan;173(1):23–27. doi: 10.1128/jb.173.1.23-27.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Atkinson M. R., Wray L. V., Jr, Fisher S. H. Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis. J Bacteriol. 1990 Sep;172(9):4758–4765. doi: 10.1128/jb.172.9.4758-4765.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chambers S. P., Prior S. E., Barstow D. A., Minton N. P. The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene. 1988 Aug 15;68(1):139–149. doi: 10.1016/0378-1119(88)90606-3. [DOI] [PubMed] [Google Scholar]
  5. Fisher S. H., Rohrer K., Ferson A. E. Role of CodY in regulation of the Bacillus subtilis hut operon. J Bacteriol. 1996 Jul;178(13):3779–3784. doi: 10.1128/jb.178.13.3779-3784.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gardan R., Rapoport G., Débarbouillé M. Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis. J Mol Biol. 1995 Jun 23;249(5):843–856. doi: 10.1006/jmbi.1995.0342. [DOI] [PubMed] [Google Scholar]
  7. Glaser P., Danchin A., Kunst F., Zuber P., Nakano M. M. Identification and isolation of a gene required for nitrate assimilation and anaerobic growth of Bacillus subtilis. J Bacteriol. 1995 Feb;177(4):1112–1115. doi: 10.1128/jb.177.4.1112-1115.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Glaser P., Kunst F., Arnaud M., Coudart M. P., Gonzales W., Hullo M. F., Ionescu M., Lubochinsky B., Marcelino L., Moszer I. Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees. Mol Microbiol. 1993 Oct;10(2):371–384. [PubMed] [Google Scholar]
  9. Jabri E., Carr M. B., Hausinger R. P., Karplus P. A. The crystal structure of urease from Klebsiella aerogenes. Science. 1995 May 19;268(5213):998–1004. [PubMed] [Google Scholar]
  10. Maeda M., Hidaka M., Nakamura A., Masaki H., Uozumi T. Cloning, sequencing, and expression of thermophilic Bacillus sp. strain TB-90 urease gene complex in Escherichia coli. J Bacteriol. 1994 Jan;176(2):432–442. doi: 10.1128/jb.176.2.432-442.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mobley H. L., Island M. D., Hausinger R. P. Molecular biology of microbial ureases. Microbiol Rev. 1995 Sep;59(3):451–480. doi: 10.1128/mr.59.3.451-480.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mulrooney S. B., Hausinger R. P. Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation. J Bacteriol. 1990 Oct;172(10):5837–5843. doi: 10.1128/jb.172.10.5837-5843.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Park I. S., Hausinger R. P. Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis. Protein Sci. 1993 Jun;2(6):1034–1041. doi: 10.1002/pro.5560020616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vogels G. D., Van der Drift C. Degradation of purines and pyrimidines by microorganisms. Bacteriol Rev. 1976 Jun;40(2):403–468. doi: 10.1128/br.40.2.403-468.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wray L. V., Jr, Ferson A. E., Rohrer K., Fisher S. H. TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8841–8845. doi: 10.1073/pnas.93.17.8841. [DOI] [PMC free article] [PubMed] [Google Scholar]

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