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. 1997 Jun;179(12):3989–3996. doi: 10.1128/jb.179.12.3989-3996.1997

Mutations in sdh (succinate dehydrogenase genes) alter the thiamine requirement of Salmonella typhimurium.

J L Enos-Berlage 1, D M Downs 1
PMCID: PMC179209  PMID: 9190816

Abstract

Mutants lacking the first enzyme in de novo purine synthesis (PurF) can synthesize thiamine if increased levels of pantothenate are present in the culture medium (J. L. Enos-Berlage and D. M. Downs, J. Bacteriol. 178:1476-1479, 1996). Derivatives of purF mutants that no longer required pantothenate for thiamine-independent growth were isolated. Analysis of these mutants demonstrated that they were defective in succinate dehydrogenase (Sdh), an enzyme of the tricarboxylic acid cycle. Results of phenotypic analyses suggested that a defect in Sdh decreased the thiamine requirement of Salmonella typhimurium. This reduced requirement correlated with levels of succinyl-coenzyme A (succinyl-CoA), which is synthesized in a thiamine pyrophosphate-dependent reaction. The effect of succinyl-CoA on thiamine metabolism was distinct from the role of pantothenate in thiamine synthesis.

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Selected References

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  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Berkowitz D., Hushon J. M., Whitfield H. J., Jr, Roth J., Ames B. N. Procedure for identifying nonsense mutations. J Bacteriol. 1968 Jul;96(1):215–220. doi: 10.1128/jb.96.1.215-220.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Castilho B. A., Olfson P., Casadaban M. J. Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol. 1984 May;158(2):488–495. doi: 10.1128/jb.158.2.488-495.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Creaghan I. T., Guest J. R. Succinate dehydrogenase-dependent nutritional requirement for succinate in mutants of Escherichia coli K12. J Gen Microbiol. 1978 Jul;107(1):1–13. doi: 10.1099/00221287-107-1-1. [DOI] [PubMed] [Google Scholar]
  5. Creaghan I. T., Guest J. R. Suppression of the succinate requirement of lipoamide dehydrogenase mutants of Escherichia coli by mutations affecting succinate dehydrogenase activity. J Gen Microbiol. 1977 Sep;102(1):183–194. doi: 10.1099/00221287-102-1-183. [DOI] [PubMed] [Google Scholar]
  6. DeMoll E., Shive W. Determination of the metabolic origin of the sulfur atom in thiamin of Escherichia coli by mass spectrometry. Biochem Biophys Res Commun. 1985 Oct 15;132(1):217–222. doi: 10.1016/0006-291x(85)91010-1. [DOI] [PubMed] [Google Scholar]
  7. Downs D. M. Evidence for a new, oxygen-regulated biosynthetic pathway for the pyrimidine moiety of thiamine in Salmonella typhimurium. J Bacteriol. 1992 Mar;174(5):1515–1521. doi: 10.1128/jb.174.5.1515-1521.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Downs D. M., Petersen L. apbA, a new genetic locus involved in thiamine biosynthesis in Salmonella typhimurium. J Bacteriol. 1994 Aug;176(16):4858–4864. doi: 10.1128/jb.176.16.4858-4864.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Downs D. M., Roth J. R. Synthesis of thiamine in Salmonella typhimurium independent of the purF function. J Bacteriol. 1991 Oct;173(20):6597–6604. doi: 10.1128/jb.173.20.6597-6604.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Enos-Berlage J. L., Downs D. M. Involvement of the oxidative pentose phosphate pathway in thiamine biosynthesis in Salmonella typhimurium. J Bacteriol. 1996 Mar;178(5):1476–1479. doi: 10.1128/jb.178.5.1476-1479.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Estramareix B., David S. Conversion of 5-aminoimidazole ribotide to the pyrimidine of thiamin in enterobacteria: study of the pathway with specifically labeled samples of riboside. Biochim Biophys Acta. 1990 Aug 17;1035(2):154–160. doi: 10.1016/0304-4165(90)90110-i. [DOI] [PubMed] [Google Scholar]
  12. Estramareix B., Therisod M. La tyrosine, facteur de la biosynthèse du thiazole de la thiamine chez Escherichia coli. Biochim Biophys Acta. 1972 Jul 19;273(2):275–282. [PubMed] [Google Scholar]
  13. Guest J. R. Partial replacement of succinate dehydrogenase function by phage- and plasmid-specified fumarate reductase in Escherichia coli. J Gen Microbiol. 1981 Feb;122(2):171–179. doi: 10.1099/00221287-122-2-171. [DOI] [PubMed] [Google Scholar]
  14. Hong J. S., Ames B. N. Localized mutagenesis of any specific small region of the bacterial chromosome. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158–3162. doi: 10.1073/pnas.68.12.3158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Langley D., Guest J. R. Biochemical and genetic characterics of deletion and other mutant strains of Salmonella typhimurium LT2 lacking alpha-keto acid dehydrogenase complex activities,. J Gen Microbiol. 1974 Jun;82(2):319–335. doi: 10.1099/00221287-82-2-319. [DOI] [PubMed] [Google Scholar]
  16. Langley D., Guest J. R. Biochemical genetics of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: genetic characterization and regulatory properties of deletion mutants. J Gen Microbiol. 1978 May;106(1):103–117. doi: 10.1099/00221287-106-1-103. [DOI] [PubMed] [Google Scholar]
  17. Liu S. L., Sanderson K. E. A physical map of the Salmonella typhimurium LT2 genome made by using XbaI analysis. J Bacteriol. 1992 Mar;174(5):1662–1672. doi: 10.1128/jb.174.5.1662-1672.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Petersen L., Downs D. M. Mutations in apbC (mrp) prevent function of the alternative pyrimidine biosynthetic pathway in Salmonella typhimurium. J Bacteriol. 1996 Oct;178(19):5676–5682. doi: 10.1128/jb.178.19.5676-5682.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Petersen L., Enos-Berlage J., Downs D. M. Genetic analysis of metabolic crosstalk and its impact on thiamine synthesis in Salmonella typhimurium. Genetics. 1996 May;143(1):37–44. doi: 10.1093/genetics/143.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schmieger H. Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet. 1972;119(1):75–88. doi: 10.1007/BF00270447. [DOI] [PubMed] [Google Scholar]
  22. Smith M. W., Neidhardt F. C. 2-Oxoacid dehydrogenase complexes of Escherichia coli: cellular amounts and patterns of synthesis. J Bacteriol. 1983 Oct;156(1):81–88. doi: 10.1128/jb.156.1.81-88.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Spencer M. E., Guest J. R. Isolation and properties of fumarate reductase mutants of Escherichia coli. J Bacteriol. 1973 May;114(2):563–570. doi: 10.1128/jb.114.2.563-570.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Way J. C., Davis M. A., Morisato D., Roberts D. E., Kleckner N. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene. 1984 Dec;32(3):369–379. doi: 10.1016/0378-1119(84)90012-x. [DOI] [PubMed] [Google Scholar]
  25. Webb E., Febres F., Downs D. M. Thiamine pyrophosphate (TPP) negatively regulates transcription of some thi genes of Salmonella typhimurium. J Bacteriol. 1996 May;178(9):2533–2538. doi: 10.1128/jb.178.9.2533-2538.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wilde R. J., Guest J. R. Transcript analysis of the citrate synthase and succinate dehydrogenase genes of Escherichia coli K12. J Gen Microbiol. 1986 Dec;132(12):3239–3251. doi: 10.1099/00221287-132-12-3239. [DOI] [PubMed] [Google Scholar]
  27. Yamauchi S., Kojima M., Nakayama F. Preparation and antigenic property of 3-dehydrolithocholylglycine 3-(O-carboxymethyl) oxime-bovine serum albumin conjugate. Steroids. 1983 Feb;41(2):165–172. doi: 10.1016/0039-128x(83)90005-3. [DOI] [PubMed] [Google Scholar]
  28. Zilles J. L., Downs D. M. A novel involvement of the PurG and PurI proteins in thiamine synthesis via the alternative pyrimidine biosynthetic (APB) pathway in Salmonella typhimurium. Genetics. 1996 Nov;144(3):883–892. doi: 10.1093/genetics/144.3.883. [DOI] [PMC free article] [PubMed] [Google Scholar]

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