Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1997 Jun;179(12):4071–4074. doi: 10.1128/jb.179.12.4071-4074.1997

Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis.

S Trinh 1, G Reysset 1
PMCID: PMC179223  PMID: 9190830

Abstract

The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host factor binding sites. The product of the mobilization gene mob421 (42.2 kDa) is a member of the Bacteroides mobilization protein family, which includes the MobA of pBI143, NBUs, and Tn4555. Sequence similarity suggests that it has both oriT binding and nicking activities. The transfer frequency of pIP421 in a B. fragilis donor strain possessing a Tc(r) or Tc(r) Em(r)-like conjugative transposon was significantly enhanced by tetracycline. Moreover, the mobilization region of pIP421 confers the ability to be mobilized from Escherichia coli by an IncP plasmid.

Full Text

The Full Text of this article is available as a PDF (163.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Assinder S. J., de Marco P., Sayers J. R., Shaw L. E., Winson M. K., Williams P. A. Identical resolvases are encoded by Pseudomonas TOL plasmids pWW53 and pDK1. Nucleic Acids Res. 1992 Oct 25;20(20):5476–5476. doi: 10.1093/nar/20.20.5476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Breuil J., Dublanchet A., Truffaut N., Sebald M. Transferable 5-nitroimidazole resistance in the Bacteroides fragilis group. Plasmid. 1989 Mar;21(2):151–154. doi: 10.1016/0147-619x(89)90060-7. [DOI] [PubMed] [Google Scholar]
  3. Dodd H. M., Bennett P. M. The R46 site-specific recombination system is a homologue of the Tn3 and gamma delta (Tn1000) cointegrate resolution system. J Gen Microbiol. 1987 Aug;133(8):2031–2039. doi: 10.1099/00221287-133-8-2031. [DOI] [PubMed] [Google Scholar]
  4. Egel-Mitani M., Flygenring H. P., Hansen M. T. A novel aspartyl protease allowing KEX2-independent MF alpha propheromone processing in yeast. Yeast. 1990 Mar-Apr;6(2):127–137. doi: 10.1002/yea.320060206. [DOI] [PubMed] [Google Scholar]
  5. Friedman D. I. Integration host factor: a protein for all reasons. Cell. 1988 Nov 18;55(4):545–554. doi: 10.1016/0092-8674(88)90213-9. [DOI] [PubMed] [Google Scholar]
  6. Haggoud A., Trinh S., Moumni M., Reysset G. Genetic analysis of the minimal replicon of plasmid pIP417 and comparison with the other encoding 5-nitroimidazole resistance plasmids from Bacteroides spp. Plasmid. 1995 Sep;34(2):132–143. doi: 10.1006/plas.1995.9994. [DOI] [PubMed] [Google Scholar]
  7. Hecht D. W., Jagielo T. J., Malamy M. H. Conjugal transfer of antibiotic resistance factors in Bacteroides fragilis: the btgA and btgB genes of plasmid pBFTM10 are required for its transfer from Bacteroides fragilis and for its mobilization by IncP beta plasmid R751 in Escherichia coli. J Bacteriol. 1991 Dec;173(23):7471–7480. doi: 10.1128/jb.173.23.7471-7480.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Li L. Y., Shoemaker N. B., Salyers A. A. Characterization of the mobilization region of a Bacteroides insertion element (NBU1) that is excised and transferred by Bacteroides conjugative transposons. J Bacteriol. 1993 Oct;175(20):6588–6598. doi: 10.1128/jb.175.20.6588-6598.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Li L. Y., Shoemaker N. B., Wang G. R., Cole S. P., Hashimoto M. K., Wang J., Salyers A. A. The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. J Bacteriol. 1995 Jul;177(14):3940–3945. doi: 10.1128/jb.177.14.3940-3945.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mahillon J., Seurinck J. Complete nucleotide sequence of pGI2, a Bacillus thuringiensis plasmid containing Tn4430. Nucleic Acids Res. 1988 Dec 23;16(24):11827–11828. doi: 10.1093/nar/16.24.11827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murphy C. G., Malamy M. H. Characterization of a "mobilization cassette" in transposon Tn4399 from Bacteroides fragilis. J Bacteriol. 1993 Sep;175(18):5814–5823. doi: 10.1128/jb.175.18.5814-5823.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy C. G., Malamy M. H. Requirements for strand- and site-specific cleavage within the oriT region of Tn4399, a mobilizing transposon from Bacteroides fragilis. J Bacteriol. 1995 Jun;177(11):3158–3165. doi: 10.1128/jb.177.11.3158-3165.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Novicki T. J., Hecht D. W. Characterization and DNA sequence of the mobilization region of pLV22a from Bacteroides fragilis. J Bacteriol. 1995 Aug;177(15):4466–4473. doi: 10.1128/jb.177.15.4466-4473.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pansegrau W., Lanka E., Barth P. T., Figurski D. H., Guiney D. G., Haas D., Helinski D. R., Schwab H., Stanisich V. A., Thomas C. M. Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis. J Mol Biol. 1994 Jun 24;239(5):623–663. doi: 10.1006/jmbi.1994.1404. [DOI] [PubMed] [Google Scholar]
  15. Privitera G., Dublanchet A., Sebald M. Transfer of multiple antibiotic resistance between subspecies of Bacteroides fragilis. J Infect Dis. 1979 Jan;139(1):97–101. doi: 10.1093/infdis/139.1.97. [DOI] [PubMed] [Google Scholar]
  16. Reysset G. Genetics of 5-nitroimidazole resistance in Bacteroides species. Anaerobe. 1996 Apr;2(2):59–69. doi: 10.1006/anae.1996.0008. [DOI] [PubMed] [Google Scholar]
  17. Reysset G., Haggoud A., Sebald M. Genetics of resistance of Bacteroides species to 5-nitroimidazole. Clin Infect Dis. 1993 Jun;16 (Suppl 4):S401–S403. doi: 10.1093/clinids/16.supplement_4.s401. [DOI] [PubMed] [Google Scholar]
  18. Reysset G., Haggoud A., Su W. J., Sebald M. Genetic and molecular analysis of pIP417 and pIP419: Bacteroides plasmids encoding 5-nitroimidazole resistance. Plasmid. 1992 May;27(3):181–190. doi: 10.1016/0147-619x(92)90020-b. [DOI] [PubMed] [Google Scholar]
  19. Salyers A. A., Shoemaker N. B., Li L. Y. In the driver's seat: the Bacteroides conjugative transposons and the elements they mobilize. J Bacteriol. 1995 Oct;177(20):5727–5731. doi: 10.1128/jb.177.20.5727-5731.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Salyers A. A., Shoemaker N. B., Stevens A. M., Li L. Y. Conjugative transposons: an unusual and diverse set of integrated gene transfer elements. Microbiol Rev. 1995 Dec;59(4):579–590. doi: 10.1128/mr.59.4.579-590.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith C. J., Parker A. C. A gene product related to Tral is required for the mobilization of Bacteroides mobilizable transposons and plasmids. Mol Microbiol. 1996 May;20(4):741–750. doi: 10.1111/j.1365-2958.1996.tb02513.x. [DOI] [PubMed] [Google Scholar]
  22. Smith C. J., Rollins L. A., Parker A. C. Nucleotide sequence determination and genetic analysis of the Bacteroides plasmid, pBI143. Plasmid. 1995 Nov;34(3):211–222. doi: 10.1006/plas.1995.0007. [DOI] [PubMed] [Google Scholar]
  23. Trinh S., Haggoud A., Reysset G. Conjugal transfer of the 5-nitroimidazole resistance plasmid pIP417 from Bacteroides vulgatus BV-17: characterization and nucleotide sequence analysis of the mobilization region. J Bacteriol. 1996 Dec;178(23):6671–6676. doi: 10.1128/jb.178.23.6671-6676.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Trinh S., Haggoud A., Reysset G., Sebald M. Plasmids pIP419 and pIP421 from Bacteroides: 5-nitroimidazole resistance genes and their upstream insertion sequence elements. Microbiology. 1995 Apr;141(Pt 4):927–935. doi: 10.1099/13500872-141-4-927. [DOI] [PubMed] [Google Scholar]
  25. Weisburg W. G., Oyaizu Y., Oyaizu H., Woese C. R. Natural relationship between bacteroides and flavobacteria. J Bacteriol. 1985 Oct;164(1):230–236. doi: 10.1128/jb.164.1.230-236.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES