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. 1997 Jul;179(13):4399–4402. doi: 10.1128/jb.179.13.4399-4402.1997

Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium.

E Webb 1, K Claas 1, D M Downs 1
PMCID: PMC179266  PMID: 9209060

Abstract

Thiamine pyrophosphate (TPP) is a required cofactor in Salmonella typhimurium that is generated de novo by the condensation of 4-amino-5-hydroxymethyl pyrimidine (HMP) pyrophosphate and 4-methyl-5-(beta-hydroxyethyl)-thiazole (THZ) monophosphate. The THZ and HMP moieties are independently synthesized, and labeling studies have demonstrated probable metabolic precursors to both. We present herein the initial characterization of thiI, a gene required for THZ synthesis. We show that thiI is a 1,449-bp open reading frame located at minute 9.65 on the S. typhimurium chromosome and that it encodes a 483-amino-acid protein with a predicted molecular mass of 55 kDa. Unlike genes in the thiamine biosynthetic operon at minute 90, thiI is not transcriptionally regulated by TPP.

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Selected References

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