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. 1997 Jul;179(13):4426–4428. doi: 10.1128/jb.179.13.4426-4428.1997

Versatile insertion plasmids for targeted genome manipulations in bacteria: isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome.

G Pósfai 1, M D Koob 1, H A Kirkpatrick 1, F R Blattner 1
PMCID: PMC179272  PMID: 9209066

Abstract

A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonuclease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli O157:H7 genome was excised and isolated in vitro, deleted in vivo, rescued as a plasmid, and transferred into another strain.

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Selected References

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