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. 1997 Jul;179(13):4446–4450. doi: 10.1128/jb.179.13.4446-4450.1997

Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine.

S L Otten 1, M A Gallo 1, K Madduri 1, X Liu 1, C R Hutchinson 1
PMCID: PMC179277  PMID: 9209071

Abstract

Characterization of the dnmZ, dnmU, and dnmV genes from the daunorubicin-producer Streptomyces peucetius by DNA sequence analysis indicated that these genes encode a protein of unknown function plus a putative thymidine diphospho-4-keto-6-deoxyglucose-3(5)-epimerase and thymidine diphospho-4-ketodeoxyhexulose reductase, respectively. Inactivation of each of the three genes by gene disruption and replacement in the wild-type strain demonstrated that all of them are required for daunosamine biosynthesis.

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Selected References

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