Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Bacteriology logoLink to Journal of Bacteriology
. 1997 Jul;179(14):4553–4558. doi: 10.1128/jb.179.14.4553-4558.1997

Occurrence of deletions, associated with genetic instability in Streptomyces ambofaciens, is independent of the linearity of the chromosomal DNA.

G Fischer 1, B Decaris 1, P Leblond 1
PMCID: PMC179291  PMID: 9226265

Abstract

The chromosomal structures of mutant strains of Streptomyces ambofaciens which have arisen from genetic instability were investigated by using pulsed-field gel electrophoresis and probing with sequences cloned from the unstable region which maps near the ends of the linear chromosomal DNA. The chromosomal structures of seven mutant strains harboring large deletions were classified into three types. (i) Deletions internal to one chromosomal arm were characterized in two of the mutant strains. In these strains, a linear chromosomal structure was retained, as were parts of the terminal inverted repeats sequences (TIRs) and the proteins bound to them. (ii) Four of the mutants presented a deletion including all sequences from the TIRs. A junction fragment homologous to sequences originating from internal region of both arms was characterized. Consequently, the chromosomal DNA of these strains was deduced to be circularized. Furthermore, chromosomal stability was assessed in the progeny of these circular DNA mutants. Additional deletion events were detected in 11 mutants among the 13 strains isolated, demonstrating that circular chromosomes do not correspond to a stabilization of the chromosome structure and that the occurrence of deletion could be independent of the presence of chromosomal ends. (iii) A mutant with DNA amplification was shown to have a linear chromosome with a deletion of all sequences between the amplified region and the end of the chromosome. The other chromosomal arm remained unaffected by deletion and associated with protein.

Full Text

The Full Text of this article is available as a PDF (510.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altenbuchner J., Cullum J. Structure of an amplifiable DNA sequence in Streptomyces lividans 66. Mol Gen Genet. 1985;201(2):192–197. doi: 10.1007/BF00425659. [DOI] [PubMed] [Google Scholar]
  2. Aubert M., Weber E., Schneider D., Simonet J. M., Decaris B. Primary structure analysis of a duplicated region in the amplifiable AUD6 locus of Streptomyces ambofaciens DSM40697. FEMS Microbiol Lett. 1993 Oct 1;113(1):49–56. doi: 10.1111/j.1574-6968.1993.tb06486.x. [DOI] [PubMed] [Google Scholar]
  3. Birch A., Häusler A., Rüttener C., Hütter R. Chromosomal deletion and rearrangement in Streptomyces glaucescens. J Bacteriol. 1991 Jun;173(11):3531–3538. doi: 10.1128/jb.173.11.3531-3538.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chu G., Vollrath D., Davis R. W. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. doi: 10.1126/science.3538420. [DOI] [PubMed] [Google Scholar]
  5. Dyson P., Schrempf H. Genetic instability and DNA amplification in Streptomyces lividans 66. J Bacteriol. 1987 Oct;169(10):4796–4803. doi: 10.1128/jb.169.10.4796-4803.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gravius B., Bezmalinović T., Hranueli D., Cullum J. Genetic instability and strain degeneration in Streptomyces rimosus. Appl Environ Microbiol. 1993 Jul;59(7):2220–2228. doi: 10.1128/aem.59.7.2220-2228.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hornemann U., Otto C. J., Zhang X. Y. DNA amplification in Streptomyces achromogenes subsp. rubradiris is accompanied by a deletion, and the amplified sequences are conditionally stable and can be eliminated by two pathways. J Bacteriol. 1989 Nov;171(11):5817–5822. doi: 10.1128/jb.171.11.5817-5822.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kieser H. M., Kieser T., Hopwood D. A. A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome. J Bacteriol. 1992 Sep;174(17):5496–5507. doi: 10.1128/jb.174.17.5496-5507.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leblond P., Demuyter P., Moutier L., Laakel M., Decaris B., Simonet J. M. Hypervariability, a new phenomenon of genetic instability, related to DNA amplification in Streptomyces ambofaciens. J Bacteriol. 1989 Jan;171(1):419–423. doi: 10.1128/jb.171.1.419-423.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leblond P., Demuyter P., Simonet J. M., Decaris B. Genetic instability and associated genome plasticity in Streptomyces ambofaciens: pulsed-field gel electrophoresis evidence for large DNA alterations in a limited genomic region. J Bacteriol. 1991 Jul;173(13):4229–4233. doi: 10.1128/jb.173.13.4229-4233.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leblond P., Fischer G., Francou F. X., Berger F., Guérineau M., Decaris B. The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA. Mol Microbiol. 1996 Jan;19(2):261–271. doi: 10.1046/j.1365-2958.1996.366894.x. [DOI] [PubMed] [Google Scholar]
  12. Leblond P., Redenbach M., Cullum J. Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2). J Bacteriol. 1993 Jun;175(11):3422–3429. doi: 10.1128/jb.175.11.3422-3429.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lezhava A., Mizukami T., Kajitani T., Kameoka D., Redenbach M., Shinkawa H., Nimi O., Kinashi H. Physical map of the linear chromosome of Streptomyces griseus. J Bacteriol. 1995 Nov;177(22):6492–6498. doi: 10.1128/jb.177.22.6492-6498.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lin Y. S., Kieser H. M., Hopwood D. A., Chen C. W. The chromosomal DNA of Streptomyces lividans 66 is linear. Mol Microbiol. 1993 Dec;10(5):923–933. doi: 10.1111/j.1365-2958.1993.tb00964.x. [DOI] [PubMed] [Google Scholar]
  15. PRIDHAM T. G., ANDERSON P., FOLEY C., LINDENFELSER L. A., HESSELTINE C. W., BENEDICT R. G. A selection of media for maintenance and taxonomic study of Streptomyces. Antibiot Annu. 1956:947–953. [PubMed] [Google Scholar]
  16. Rauland U., Glocker I., Redenbach M., Cullum J. DNA amplifications and deletions in Streptomyces lividans 66 and the loss of one end of the linear chromosome. Mol Gen Genet. 1995 Jan 6;246(1):37–44. doi: 10.1007/BF00290131. [DOI] [PubMed] [Google Scholar]
  17. Redenbach M., Flett F., Piendl W., Glocker I., Rauland U., Wafzig O., Kliem R., Leblond P., Cullum J. The Streptomyces lividans 66 chromosome contains a 1 MB deletogenic region flanked by two amplifiable regions. Mol Gen Genet. 1993 Nov;241(3-4):255–262. doi: 10.1007/BF00284676. [DOI] [PubMed] [Google Scholar]
  18. Redenbach M., Kieser H. M., Denapaite D., Eichner A., Cullum J., Kinashi H., Hopwood D. A. A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol Microbiol. 1996 Jul;21(1):77–96. doi: 10.1046/j.1365-2958.1996.6191336.x. [DOI] [PubMed] [Google Scholar]
  19. Robinson M., Lewis E., Napier E. Occurrence of reiterated DNA sequences in strains of Streptomyces produced by an interspecific protoplast fusion. Mol Gen Genet. 1981;182(2):336–340. doi: 10.1007/BF00269680. [DOI] [PubMed] [Google Scholar]
  20. Schrempf H. Deletion and amplification of DNA sequences in melanin-negative variants of Streptomyces reticuli. Mol Gen Genet. 1983;189(3):501–505. doi: 10.1007/BF00325917. [DOI] [PubMed] [Google Scholar]
  21. Smith G. E., Summers M. D. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Anal Biochem. 1980 Nov 15;109(1):123–129. doi: 10.1016/0003-2697(80)90019-6. [DOI] [PubMed] [Google Scholar]
  22. Young M., Cullum J. A plausible mechanism for large-scale chromosomal DNA amplification in streptomycetes. FEBS Lett. 1987 Feb 9;212(1):10–14. doi: 10.1016/0014-5793(87)81547-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES