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. 1997 Jul;179(14):4635–4638. doi: 10.1128/jb.179.14.4635-4638.1997

Transposition of the IS21-related element IS1415 in Rhodococcus erythropolis.

I Nagy 1, G Schoofs 1, J Vanderleyden 1, R De Mot 1
PMCID: PMC179304  PMID: 9226278

Abstract

Three copies of the IS21-related transposable element IS1415 were identified in Rhodococcus erythropolis NI86/21. Adjacent to one of the IS1415 copies, a 47-bp sequence nearly identical to the conserved 5' end of integrons was found. Accurate transposition of IS1415 carrying a chloramphenicol resistance gene (Tn5561) was demonstrated following delivery from a suicide vector to R. erythropolis SQ1.

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Selected References

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  1. Brown H. J., Stokes H. W., Hall R. M. The integrons In0, In2, and In5 are defective transposon derivatives. J Bacteriol. 1996 Aug;178(15):4429–4437. doi: 10.1128/jb.178.15.4429-4437.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown N. L., Misra T. K., Winnie J. N., Schmidt A., Seiff M., Silver S. The nucleotide sequence of the mercuric resistance operons of plasmid R100 and transposon Tn501: further evidence for mer genes which enhance the activity of the mercuric ion detoxification system. Mol Gen Genet. 1986 Jan;202(1):143–151. doi: 10.1007/BF00330531. [DOI] [PubMed] [Google Scholar]
  3. De Mot R., Nagy I., Schoofs G., Vanderleyden J. Sequences of the cobalamin biosynthetic genes cobK, cobL and cobM from Rhodococcus sp. NI86/21. Gene. 1994 May 27;143(1):91–93. doi: 10.1016/0378-1119(94)90610-6. [DOI] [PubMed] [Google Scholar]
  4. Denome S. A., Young K. D. Identification and activity of two insertion sequence elements in Rhodococcus sp. strain IGTS8. Gene. 1995 Aug 8;161(1):33–38. doi: 10.1016/0378-1119(95)00241-w. [DOI] [PubMed] [Google Scholar]
  5. Desomer J., Crespi M., Van Montagu M. Illegitimate integration of non-replicative vectors in the genome of Rhodococcus fascians upon electrotransformation as an insertional mutagenesis system. Mol Microbiol. 1991 Sep;5(9):2115–2124. doi: 10.1111/j.1365-2958.1991.tb02141.x. [DOI] [PubMed] [Google Scholar]
  6. Desomer J., Dhaese P., Montagu M. V. Transformation of Rhodococcus fascians by High-Voltage Electroporation and Development of R. fascians Cloning Vectors. Appl Environ Microbiol. 1990 Sep;56(9):2818–2825. doi: 10.1128/aem.56.9.2818-2825.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Desomer J., Vereecke D., Crespi M., Van Montagu M. The plasmid-encoded chloramphenicol-resistance protein of Rhodococcus fascians is homologous to the transmembrane tetracycline efflux proteins. Mol Microbiol. 1992 Aug;6(16):2377–2385. doi: 10.1111/j.1365-2958.1992.tb01412.x. [DOI] [PubMed] [Google Scholar]
  8. Dodd I. B., Egan J. B. Improved detection of helix-turn-helix DNA-binding motifs in protein sequences. Nucleic Acids Res. 1990 Sep 11;18(17):5019–5026. doi: 10.1093/nar/18.17.5019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Finnerty W. R. The biology and genetics of the genus Rhodococcus. Annu Rev Microbiol. 1992;46:193–218. doi: 10.1146/annurev.mi.46.100192.001205. [DOI] [PubMed] [Google Scholar]
  10. Freiberg C., Perret X., Broughton W. J., Rosenthal A. Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable "sequenase": a beginning. Genome Res. 1996 Jul;6(7):590–600. doi: 10.1101/gr.6.7.590. [DOI] [PubMed] [Google Scholar]
  11. Grobler J., Bauer F., Subden R. E., Van Vuuren H. J. The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids. Yeast. 1995 Dec;11(15):1485–1491. doi: 10.1002/yea.320111503. [DOI] [PubMed] [Google Scholar]
  12. Hall R. M., Brown H. J., Brookes D. E., Stokes H. W. Integrons found in different locations have identical 5' ends but variable 3' ends. J Bacteriol. 1994 Oct;176(20):6286–6294. doi: 10.1128/jb.176.20.6286-6294.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hall R. M., Collis C. M. Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination. Mol Microbiol. 1995 Feb;15(4):593–600. doi: 10.1111/j.1365-2958.1995.tb02368.x. [DOI] [PubMed] [Google Scholar]
  14. Jäger W., Schäfer A., Kalinowski J., Pühler A. Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker. FEMS Microbiol Lett. 1995 Feb 1;126(1):1–6. doi: 10.1111/j.1574-6968.1995.tb07381.x. [DOI] [PubMed] [Google Scholar]
  15. Kholodii G. Y., Mindlin S. Z., Bass I. A., Yurieva O. V., Minakhina S. V., Nikiforov V. G. Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an integron. Mol Microbiol. 1995 Sep;17(6):1189–1200. doi: 10.1111/j.1365-2958.1995.mmi_17061189.x. [DOI] [PubMed] [Google Scholar]
  16. Komeda H., Kobayashi M., Shimizu S. Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1. Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4267–4272. doi: 10.1073/pnas.93.9.4267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Koonin E. V. DnaC protein contains a modified ATP-binding motif and belongs to a novel family of ATPases including also DnaA. Nucleic Acids Res. 1992 Apr 25;20(8):1997–1997. doi: 10.1093/nar/20.8.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nagy I., Compernolle F., Ghys K., Vanderleyden J., De Mot R. A single cytochrome P-450 system is involved in degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine by Rhodococcus sp. strain NI86/21. Appl Environ Microbiol. 1995 May;61(5):2056–2060. doi: 10.1128/aem.61.5.2056-2060.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nagy I., Schoofs G., Compernolle F., Proost P., Vanderleyden J., de Mot R. Degradation of the thiocarbamate herbicide EPTC (S-ethyl dipropylcarbamothioate) and biosafening by Rhodococcus sp. strain NI86/21 involve an inducible cytochrome P-450 system and aldehyde dehydrogenase. J Bacteriol. 1995 Feb;177(3):676–687. doi: 10.1128/jb.177.3.676-687.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Picardeau M., Varnerot A., Rauzier J., Gicquel B., Vincent V. Mycobacterium xenopi IS1395, a novel insertion sequence expanding the IS256 family. Microbiology. 1996 Sep;142(Pt 9):2453–2461. doi: 10.1099/00221287-142-9-2453. [DOI] [PubMed] [Google Scholar]
  21. Polard P., Chandler M. Bacterial transposases and retroviral integrases. Mol Microbiol. 1995 Jan;15(1):13–23. doi: 10.1111/j.1365-2958.1995.tb02217.x. [DOI] [PubMed] [Google Scholar]
  22. Recchia G. D., Hall R. M. Gene cassettes: a new class of mobile element. Microbiology. 1995 Dec;141(Pt 12):3015–3027. doi: 10.1099/13500872-141-12-3015. [DOI] [PubMed] [Google Scholar]
  23. Reimmann C., Moore R., Little S., Savioz A., Willetts N. S., Haas D. Genetic structure, function and regulation of the transposable element IS21. Mol Gen Genet. 1989 Feb;215(3):416–424. doi: 10.1007/BF00427038. [DOI] [PubMed] [Google Scholar]
  24. Rådström P., Sköld O., Swedberg G., Flensburg J., Roy P. H., Sundström L. Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements. J Bacteriol. 1994 Jun;176(11):3257–3268. doi: 10.1128/jb.176.11.3257-3268.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shields D. C., Higgins D. G., Sharp P. M. GCWIND: a microcomputer program for identifying open reading frames according to codon positional G+C content. Comput Appl Biosci. 1992 Oct;8(5):521–523. doi: 10.1093/bioinformatics/8.5.521. [DOI] [PubMed] [Google Scholar]
  26. Solinas F., Marconi A. M., Ruzzi M., Zennaro E. Characterization and sequence of a novel insertion sequence, IS1162, from Pseudomonas fluorescens. Gene. 1995 Mar 21;155(1):77–82. doi: 10.1016/0378-1119(94)00922-f. [DOI] [PubMed] [Google Scholar]
  27. Tamura T., Nagy I., Lupas A., Lottspeich F., Cejka Z., Schoofs G., Tanaka K., De Mot R., Baumeister W. The first characterization of a eubacterial proteasome: the 20S complex of Rhodococcus. Curr Biol. 1995 Jul 1;5(7):766–774. doi: 10.1016/s0960-9822(95)00153-9. [DOI] [PubMed] [Google Scholar]
  28. Warhurst A. M., Fewson C. A. Biotransformations catalyzed by the genus Rhodococcus. Crit Rev Biotechnol. 1994;14(1):29–73. doi: 10.3109/07388559409079833. [DOI] [PubMed] [Google Scholar]
  29. Yamada H., Kobayashi M. Nitrile hydratase and its application to industrial production of acrylamide. Biosci Biotechnol Biochem. 1996 Sep;60(9):1391–1400. doi: 10.1271/bbb.60.1391. [DOI] [PubMed] [Google Scholar]

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