Abstract
Aims/hypothesis
To assess whether self-monitoring of blood glucose (SMBG) is an independent predictor of improved outcome in a community-based cohort of type 2 diabetic patients.
Materials and methods
We used longitudinal data from (1) 1,280 type 2 diabetic participants in the observational Fremantle Diabetes Study (FDS) who reported SMBG and diabetes treatment status at study entry (1993–1996), and (2) a subset of 531 participants who attended six or more annual assessments (referred to as the 5-year cohort). Diabetes-related morbidity, cardiac death and all-cause mortality were ascertained at each assessment, supplemented by linkage to the Western Australian Data Linkage System.
Results
At baseline, 70.2% (898 out of 1,280) of type 2 patients used SMBG. During 12,491 patient-years of follow-up (mean 9.8 ± 3.5 years), 486 (38.0%) type 2 participants died (196 [15.3%] from cardiac causes). SMBG was significantly less prevalent in those who died during follow-up than in those who were still alive at the end of June 2006 (65.4 vs 73.0%, p = 0.005). In Cox proportional hazards modelling, after adjustment for confounding and explanatory variables, SMBG was not independently associated with all-cause mortality, but was associated with a 79% increased risk of cardiovascular mortality in patients not treated with insulin. For the 5-year cohort, time-dependent SMBG was independently associated with a 48% reduced risk of retinopathy.
Conclusions/interpretation
SMBG was not independently associated with improved survival. Inconsistent findings relating to the association of SMBG with cardiac death and retinopathy may be due to confounding, incomplete covariate adjustment or chance.
Keywords: Cohort study, Epidemiology, Outcomes, Self-monitoring of blood glucose, Type 2 diabetes
Introduction
Intensive glycaemic control is a cost-effective way of reducing the complications associated with type 2 diabetes [1]. Whether self-monitoring of blood glucose (SMBG) can improve glycaemia is subject to debate [2, 3]. The Retrolective Study ‘Self-monitoring of Blood Glucose and Outcome in Patients with Type 2 Diabetes’ (ROSSO) investigators recently reported that SMBG was associated with decreased diabetes-related morbidity and all-cause mortality in type 2 diabetes, despite the SMBG group having a higher mean baseline fasting plasma glucose than the non-SMBG group [4]. In an Australian community-based cohort of type 2 patients we found that SMBG was not a determinant of glycaemic control [5], and now investigate whether, as in ROSSO, those who performed SMBG had better outcomes than those who did not.
Subjects and methods
Subjects The Fremantle Diabetes Study (FDS) was a longitudinal observational study in a community of 120,097 people in the state of Western Australia. We identified 2,258 subjects between 1993 and 1996, using all available sources, and recruited 1,426 (63%) to attend annual assessments, of whom 1,294 (91%) had type 2 diabetes [6]. The FDS protocol was approved by the Fremantle Hospital Human Rights Committee and all subjects gave informed consent. The present study included (1) 1,280 type 2 patients (mean age 64.1 ± 11.3 years, 48.8% men) with complete diabetes treatment and mortality data who reported SMBG status at FDS entry, and (2) a subset of 531 patients (mean age 62.4 ± 9.4 years, 54.2% men) who attended six or more consecutive annual assessments.
Clinical assessment At baseline and annual reviews, a comprehensive history was taken and a physical examination was performed. Complications were identified using standard criteria [5, 7]. Microalbuminuria was defined as an urinary albumin:creatinine ratio (ACR) ≥3.0 mg/mmol on a first morning sample, neuropathy as a score >2/8 on the clinical portion of the Michigan Neuropathy Screening Instrument, and retinopathy as any grade in one/both eyes on direct and/or indirect ophthalmoscopy and/or detailed specialist assessment. Self-report and hospitalisations were used to identify cerebrovascular disease (CVD; stroke, transient ischaemic attack) and CHD (myocardial infarction, angina, coronary revascularisation). Peripheral arterial disease (PAD) was defined as an ankle:brachial index ≤0.9 or diabetes-related amputation. Blindness in one or both eyes, foot amputation and end-stage renal disease were also defined to allow comparison with the ROSSO findings [4].
Hospital morbidity and mortality Western Australian government registers record details of all deaths and hospital admissions, and make up part of the Western Australian Data Linkage System (WADLS) [8], which provided endpoint data to the end of June 2006. The Confidentiality of Health Information Committee approved linkage with the FDS database. All hospitalisations for endpoints were identified using the International Classification of Diseases (ICD)-9-CM and ICD-10-AM diagnosis/procedure codes. Causes of death were classified independently by two authors (D.G. Bruce, T.M.E. Davis) as ‘cardiac’ or ‘other’ [7].
Statistical analysis Data were analysed using SPSS for Windows (version 11.5) and are presented as proportions or means±SD. Comparison of two independent proportions was by Fisher’s exact test. Freedom from non-fatal endpoints, all-cause mortality or cardiac death was analysed by baseline SMBG status using the Kaplan–Meier method and compared with the log-rank test. For outcomes with sufficient events, Cox proportional hazards modelling (forward conditional variable entry and removal with p < 0.05 and p > 0.05, respectively), was used to determine: (1) independent predictors of first ever occurrence of endpoints, with all clinically plausible univariate variables with a p value of less than 0.20 considered for entry; and (2) whether, after adjusting for these variables, either SMBG at baseline or the time-dependent covariate SMBG was independently associated with outcome. A p value of less than 0.05 was considered significant.
Results
At baseline, 898 type 2 patients (70.2%) performed SMBG. During 12,491 patient-years of follow-up (mean 9.8 ± 3.5 years), 486 (38.0%) died, of which 196 (15.3%) deaths were from cardiac causes. SMBG was significantly less prevalent in those who died during follow-up than in those who were alive at the end of June 2006 (65.4 vs 73.0%, p = 0.005).
SMBG and mortality In unadjusted survival analysis (Model 1), SMBG was associated with a significant 24% reduction in all-cause mortality (Table 1). After adjusting for age, sex and diabetes duration (Model 2), this became a non-significant 11% increased risk. Additional adjustment for independent risk factors for all-cause mortality (Model 3) did not alter this finding. In patients on diet±oral hypoglycaemic agents (OHAs) and in those on insulin, baseline SMBG was associated with significant unadjusted 24% and 54% reductions in the risk of death, respectively, which became non-significant after full adjustment. In unadjusted models for cardiac death, there was a significant 55% risk reduction in insulin-treated patients (Table 1); after full adjustment, this became non-significant. SMBG was associated with a 55% increased risk of cardiac death in Model 3 as a result of a significant 79% increased risk in patients not treated with insulin.
Table 1.
Hazard ratios for all-cause and cardiac mortality in 1,280 type 2 diabetes patients by SMBG status for: (1) all diabetes treatment types; (2) those who were treated by diet with or without OHAs; and (3) those taking insulin with or without OHAs
No SMBG | SMBG | |||||||
---|---|---|---|---|---|---|---|---|
Number of patients | Number of events | Absolute riska | Number of patients | Number of events | Absolute riska | Log-rank p value | HR (95%CI) | |
All patients | ||||||||
All-cause mortality | ||||||||
Model 1 (unadjusted) | 382 | 168 | 46.7 | 898 | 318 | 35.8 | 0.004 | 0.76 (0.63–0.92) |
Model 2 (adjusted)b | 1.11 (0.92–1.35) | |||||||
Model 3 (adjusted)c | 1.15 (0.93–1.44) | |||||||
Cardiac mortality | ||||||||
Model 1 (unadjusted) | 382 | 61 | 17.0 | 898 | 135 | 15.2 | 0.46 | 0.89 (0.66–1.21) |
Model 2 (adjusted)b | 1.35 (0.99–1.86) | |||||||
Model 3 (adjusted)d | 1.55 (1.07–2.24) | |||||||
Diet ± OHAs | ||||||||
All-cause mortality | ||||||||
Model 1 (unadjusted) | 354 | 145 | 42.6 | 773 | 252 | 32.5 | 0.008 | 0.76 (0.62–0.93) |
Model 2 (adjusted)b | 1.15 (0.93–1.43) | |||||||
Model 3 (adjusted)e | 1.20 (0.94–1.52) | |||||||
Cardiac mortality | ||||||||
Model 1 (unadjusted) | 354 | 51 | 15.0 | 773 | 108 | 13.9 | 0.65 | 0.93 (0.66–1.29) |
Model 2 (adjusted)b | 1.51 (1.06–2.14) | |||||||
Model 3 (adjusted)f | 1.79 (1.19–2.69) | |||||||
Insulin ± OHAs | ||||||||
All-cause mortality | ||||||||
Model 1 (unadjusted) | 28 | 23 | 119.3 | 125 | 66 | 58.4 | 0.001 | 0.46 (0.29–0.75) |
Model 2 (adjusted)b | 0.68 (0.41–1.14) | |||||||
Model 3 (adjusted)g | 0.73 (0.43–1.26) | |||||||
Cardiac mortality | ||||||||
Model 1 (unadjusted) | 28 | 10 | 51.9 | 125 | 27 | 23.9 | 0.026 | 0.45 (0.22–0.93) |
Model 2 (adjusted)b | 0.58 (0.27–1.27) | |||||||
Model 3 (adjusted)h | 0.52 (0.22–1.19) |
aEvents per 1,000 person–years
bModel 2 includes age, sex and duration of diabetes in each case. Model 3 includes all variables in Model 2, plus:
cprior CHD, CVD, PAD, neuropathy, retinopathy, ln[ACR], any exercise in past 2 weeks (negative), abdominal obesity (negative), on lipid-lowering medications (negative), Australian Aboriginal, Asian (negative), current smoker
dprior CHD, CVD, PAD, neuropathy, retinopathy, ln[ACR], systolic BP (negative), total serum cholesterol, Australian Aboriginal, current smoker
eprior CHD, CVD, PAD, neuropathy, retinopathy, ln[ACR], abdominal obesity (negative), on lipid-lowering medication (negative), Australian Aboriginal, current smoker
fprior CHD, PAD, neuropathy, retinopathy, ln[ACR], systolic BP (negative), total serum cholesterol, current smoker
gprior CHD, diabetes education (ever; negative), HbA1c, Australian Aboriginal
hprior CHD, retinopathy, HbA1c, Australian Aboriginal
Five-year cohort Consistent with intensification of diabetes therapy, and as reported previously [5], the proportion of the 5-year longitudinal cohort using SMBG increased over a mean of 5.4 ± 0.5 years, from 75.2% at entry to 85.5% at third review, with little change thereafter.We ascertained the incidence of first ever occurrence of micro- and macrovascular complications by baseline SMBG status (Table 2). Unadjusted survival analysis showed no significant benefit of baseline SMBG (p ≥ 0.11) for any endpoint, single or combination. Neither adjustment for age, sex and diabetes duration (Model 2), nor relevant additional variables independently associated with the outcomes in Cox proportional hazards modelling (Model 3), improved this result. Time-dependent Cox models were included to allow for commencement (or discontinuation) of SMBG during follow-up. In both unadjusted and adjusted models, SMBG during follow-up was associated with a reduction in the risk of retinopathy of approximately 50%. In unadjusted analysis, the risk of the development of any macrovascular disease was reduced by 33% in patients who used SMBG, but this became non-significant after full adjustment.SMBG was not associated with the first occurrence of the composite ROSSO endpoint (which occurred infrequently), either at baseline or as a time-dependent covariate.
Table 2.
Hazard ratios of first-ever non-fatal complications in the 531 type 2 diabetes patients in the longitudinal arm for single and combined micro- and macrovascular events, and the ROSSO Study non-fatal endpoint, by SMBG status at: (1) baseline; and (2) each annual assessment (time-dependent covariate)
No SMBG at baseline | SMBG at baseline | Time-dependent SMBG use | |||||||
---|---|---|---|---|---|---|---|---|---|
No. of patients | No. of events | Absolute riska | No. of patients | No. of events | Absolute riska | Log-rank p value | HR (95% CI) | HR (95% CI) | |
Retinopathy | |||||||||
Model 1 (unadjusted) | 116 | 47 | 90.0 | 349 | 112 | 68.4 | 0.11 | 0.76 (0.54–1.07) | 0.51 (0.36–0.72) |
Model 2 (adjusted)b | 0.80 (0.57–1.13) | 0.52 (0.37–0.74) | |||||||
Model 3 (adjusted)c | 0.82 (0.58–1.16) | 0.52 (0.37–0.73) | |||||||
Neuropathy | |||||||||
Model 1 (unadjusted) | 84 | 62 | 199.3 | 256 | 186 | 203.0 | 0.76 | 1.05 (0.78–1.40) | 0.77 (0.57–1.03) |
Model 2 (adjusted)b | 1.16 (0.87–1.55) | 0.79 (0.59–1.07) | |||||||
Model 3 (adjusted)d | 1.30 (0.97–1.74) | 0.89 (0.66–1.20) | |||||||
Microalbuminuria | |||||||||
Model 1 (unadjusted) | 82 | 37 | 104.1 | 258 | 103 | 90.6 | 0.38 | 0.85 (0.58–1.23) | 0.77 (0.52–1.14) |
Model 2 (adjusted)b | 0.88 (0.60–1.28) | 0.78 (0.53–1.15) | |||||||
Model 3 (adjusted)e | 0.91 (0.62–1.33) | 0.74 (0.50–1.10) | |||||||
Any microangiopathy | |||||||||
Model 1 (unadjusted) | 58 | 50 | 280.5 | 172 | 153 | 302.6 | 0.59 | 1.09 (0.79–1.51) | 0.99 (0.71–1.38) |
Model 2 (adjusted)b | 1.22 (0.89–1.68) | 0.96 (0.69–1.33) | |||||||
Model 3 (adjusted)f | 1.34 (0.97–1.86) | 0.98 (0.71–1.37) | |||||||
Myocardial infarction | |||||||||
Model 1 (unadjusted) | 131 | 6 | 8.6 | 386 | 18 | 8.8 | 0.97 | 1.02 (0.41–2.57) | 0.58 (0.24–1.41) |
Model 2 (adjusted)b | 1.13 (0.44–2.89) | 0.63 (0.26–1.54) | |||||||
Stroke | |||||||||
Model 1 (unadjusted) | 133 | 2 | 2.7 | 398 | 2 | 0.9 | 0.28 | 0.35 (0.05–2.52) | 0.19 (0.03–1.36) |
Model 2 (adjusted)b | 0.82 (0.10–6.60) | 0.30 (0.04–2.28) | |||||||
Peripheral arterial disease | |||||||||
Model 1 (unadjusted) | 101 | 51 | 119.3 | 307 | 134 | 99.3 | 0.29 | 0.84 (0.61–1.16) | 0.74 (0.53–1.04) |
Model 2 (adjusted)b | 0.97 (0.70–1.36) | 0.80 (0.57–1.13) | |||||||
Model 3 (adjusted)g | 1.13 (0.79–1.62) | 0.89 (0.62–1.28) | |||||||
Any macroangiopathy | |||||||||
Model 1 (unadjusted) | 99 | 54 | 133.7 | 297 | 133 | 103.8 | 0.11 | 0.77 (0.56–1.06) | 0.67 (0.48–0.93) |
Model 2 (adjusted)b | 0.88 (0.63–1.21) | 0.71 (0.51–0.99) | |||||||
Model 3 (adjusted)h | 0.88 (0.63–1.22) | 0.74 (0.52–1.04) | |||||||
All vascular disease | |||||||||
Model 1 (unadjusted) | 44 | 41 | 222.0 | 141 | 128 | 230.1 | 0.95 | 0.99 (0.69–1.41) | 1.38 (0.89–2.15) |
Model 2 (adjusted)b | 1.15 (0.80–1.64) | 1.43 (0.91–2.23) | |||||||
Model 3 (adjusted)i | – | – | |||||||
ROSSO endpoint | |||||||||
Model 1 (unadjusted) | 110 | 8 | 13.8 | 328 | 26 | 15.3 | 0.76 | 1.13 (0.51–2.50) | 0.54 (0.26–1.14) |
Model 2 (adjusted)b | 1.29 (0.58–2.87) | 0.58 (0.27–1.23) |
aEvents per 1,000 person–years
bModel 2 includes age, sex and duration of diabetes in each case. Model 3 includes all variables in Model 2, plus:
cfasting plasma glucose
dAsian (negative), other European, mixed/other ethnicity (negative)
eBMI, ln[ACR], aspirin-use, any exercise in past 2 weeks (negative), married (negative)
fln[ACR]
gsystolic BP, aspirin-use, diabetes education (ever; negative), smoker, √daily alcohol consumption
hsystolic BP, aspirin-use, smoker, √daily alcohol consumption
inone after adjustment forb
Discussion
The present study represents the second observational assessment of the relationship between SMBG and outcome in type 2 diabetes. With regard to all-cause mortality in the full FDS sample, our unadjusted data showed that baseline SMBG was associated with a significant risk reduction, a relationship observed in both non-insulin-treated and insulin-treated subgroups. After adjustment for other potential explanatory variables, this apparent benefit was lost in both subgroups. For both combined micro- and macroangiopathy outcomes, SMBG was not associated with a first ever event in the 5-year longitudinal cohort in either unadjusted or adjusted models, with or without time-dependent analyses. These findings are inconsistent with those of the ROSSO study [4], in which all-cause mortality and diabetes-related morbidity were reduced by 32 and 51%, respectively, in patients employing SMBG.
What is the explanation for these discrepancies? ROSSO had a larger number of patients than the FDS (3,268 vs 1,280), followed for a similar period. However, while the FDS involved a prevalent, prospectively-studied, community-based cohort and all patients had access to subsidised SMBG [5], ROSSO was ‘retrolective’—newly-diagnosed patients aged ≥45 years were selected—and only those receiving insulin were reimbursed for SMBG [4]. Whereas there was a low rate of migration of the FDS sample out of Western Australia [9] (96% had been captured on the WADLS by the end of June 2006 [10]), the retention rate of the ROSSO primary care sample is unknown. The range of potential explanatory variables (including diabetes education, marital status, education, alcohol consumption, exercise and urinary ACR) and the completeness of data collection (e.g. HbA1c and serum HDL-cholesterol results from a single laboratory were available for ≥98.8% of FDS patients compared with only 45.5 and 30.2%, respectively, from multiple laboratories in ROSSO [4]) were both much greater in the FDS. The present analyses are therefore likely to have assessed the independent contribution of SMBG to diabetes-associated morbidity and mortality with greater validity. A healthy survivor effect may also have confounded the relationship between SMBG and non-fatal outcome in ROSSO, while these analyses were undertaken in FDS participants alive at the end of 5 years of follow-up.
After adjustment, SMBG was associated with an increased risk of cardiac death in patients not treated with insulin. Although the ROSSO investigators suggest that, based on apparent benefits, SMBG may be a surrogate for greater patient empowerment and physician interest, with consequently improved compliance [4], it is equally plausible that SMBG may represent a belated attempt to improve glycaemic control by mostly non-insulin-treated patients who become aware of symptoms of coronary insufficiency and heart failure. Retinopathy was the only non-fatal complication with which SMBG was independently associated, with a significantly lower relative risk in our 5-year cohort in both unadjusted and adjusted time-dependent models. This could be a chance finding, but could also reflect the effect of other variables not measured in the FDS. It is not due to better glycaemic control in patients who perform SMBG, since SMBG use did not improve glycaemia [5]. The ROSSO study report does not contain a similar disaggregated analysis [4].
The results of the present study do not support a relationship between SMBG and improved survival in a well-characterised community-based sample of type 2 diabetic patients. We found evidence that SMBG was associated with an increased risk of cardiac death and a reduced risk of retinopathy. These conflicting results might reflect complex interactions between patient, physician and disease factors in particular circumstances, but may also represent the effects of confounding, incomplete covariate adjustment or chance.
Acknowledgements
We thank FDS participants and staff. The Raine Foundation, University of Western Australia, funded the FDS.
Duality of interest We declare no current or past interest in relationship with a company/organisation that could benefit financially from the publication of our paper.
Abbreviations
- ACR
urinary albumin:creatinine ratio
- CVD
cerebrovascular disease
- FDS
Fremantle Diabetes Study
- HR
hazard ratio
- ICD
International Classification of Diseases
- OHA
oral hypoglycaemic agent
- PAD
peripheral arterial disease
- ROSSO
Retrolective Study ‘Self-monitoring of Blood Glucose and Outcome in Patients with Type 2 Diabetes’
- SMBG
self-monitoring of blood glucose
- WADLS
Western Australian Data Linkage System
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