Abstract
Interruption of folate metabolism by trimethoprim results in the elevated expression of folate stress proteins in Escherichia coli. E. coli grown in culture medium supplemented with the folate-dependent metabolites glycine, methionine, and the purine nucleoside inosine shows reduced expression of folate stress proteins. The folate stress proteins include the universal stress protein, the ferric uptake regulatory repressor, and possibly, lipoamide dehydrogenase, the L protein component of the glycine cleavage enzyme complex.
Full Text
The Full Text of this article is available as a PDF (398.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Amyes S. G., Smith J. T. Trimethoprim action and its analogy with thymine starvation. Antimicrob Agents Chemother. 1974 Feb;5(2):169–178. doi: 10.1128/aac.5.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Basso J., Herrington M. B. Changes in translational accuracy of Escherichia coli when folate metabolism is perturbed. Microbios. 1994;77(313):231–237. [PubMed] [Google Scholar]
- De Lorenzo V., Herrero M., Giovannini F., Neilands J. B. Fur (ferric uptake regulation) protein and CAP (catabolite-activator protein) modulate transcription of fur gene in Escherichia coli. Eur J Biochem. 1988 May 2;173(3):537–546. doi: 10.1111/j.1432-1033.1988.tb14032.x. [DOI] [PubMed] [Google Scholar]
- Hamm-Alvarez S. F., Sancar A., Rajagopalan K. V. The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants. J Biol Chem. 1990 Jun 15;265(17):9850–9856. [PubMed] [Google Scholar]
- Nagy P. L., Marolewski A., Benkovic S. J., Zalkin H. Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli. J Bacteriol. 1995 Mar;177(5):1292–1298. doi: 10.1128/jb.177.5.1292-1298.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nyström T., Neidhardt F. C. Cloning, mapping and nucleotide sequencing of a gene encoding a universal stress protein in Escherichia coli. Mol Microbiol. 1992 Nov;6(21):3187–3198. doi: 10.1111/j.1365-2958.1992.tb01774.x. [DOI] [PubMed] [Google Scholar]
- Nyström T., Neidhardt F. C. Effects of overproducing the universal stress protein, UspA, in Escherichia coli K-12. J Bacteriol. 1996 Feb;178(3):927–930. doi: 10.1128/jb.178.3.927-930.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
- Rohlman C. E., Matthews R. G. Role of purine biosynthetic intermediates in response to folate stress in Escherichia coli. J Bacteriol. 1990 Dec;172(12):7200–7210. doi: 10.1128/jb.172.12.7200-7210.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stauffer L. T., Fogarty S. J., Stauffer G. V. Characterization of the Escherichia coli gcv operon. Gene. 1994 May 3;142(1):17–22. doi: 10.1016/0378-1119(94)90349-2. [DOI] [PubMed] [Google Scholar]
- Steiert P. S., Stauffer L. T., Stauffer G. V. The lpd gene product functions as the L protein in the Escherichia coli glycine cleavage enzyme system. J Bacteriol. 1990 Oct;172(10):6142–6144. doi: 10.1128/jb.172.10.6142-6144.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]