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. 1997 Sep;179(18):5663–5669. doi: 10.1128/jb.179.18.5663-5669.1997

Characterization of the capsular polysaccharide of Burkholderia (Pseudomonas) pseudomallei 304b.

H Masoud 1, M Ho 1, T Schollaardt 1, M B Perry 1
PMCID: PMC179451  PMID: 9294419

Abstract

Burkholderia (Pseudomonas) pseudomallei is the causative agent of melioidosis, a bacterial infection of considerable morbidity in areas of endemicity of Southeast Asia and northern Australia. Clinical isolates of B. pseudomallei have been demonstrated to produce a lipopolysaccharide (LPS) containing two separate and chemically distinct antigenic O polysaccharides against which infected patients produced antibodies. A putative capsular polysaccharide (CPS) has also been reported and is thought to be antigenically conserved based on results of serological studies with clinical B. pseudomallei isolates. In the present study, the CPS isolated from B. pseudomallei 304b from northeastern Thailand was found to have an [alpha]D of +99 degrees (water), was composed of D-galactose (D-Gal), 3-deoxy-D-manno-2-octulosonic acid (KDO), and O-acetyl 3:1:1), and was a linear unbranched polymer of repeating tetrasaccharide units having the following structure: -3)-2-O-Ac-beta-D-Galp-(1-4)-alpha-D-Galp-(1-3)-beta-D -Galp-(1-5)-beta-D-KDOp-(2-. Sera from 13 of 15 patients with different clinical manifestations of melioidosis but not normal controls recognize the CPS, which suggests that it is immunogenic and raises the possibility that it may have a role as a vaccine candidate and/or diagnostic agent.

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Selected References

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  1. Altman E., Brisson J. R., Gagné S. M., Perry M. B. Structure of the capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5b. Eur J Biochem. 1992 Feb 15;204(1):225–230. doi: 10.1111/j.1432-1033.1992.tb16628.x. [DOI] [PubMed] [Google Scholar]
  2. Altman E., Brisson J. R., Perry M. B. Structure of the capsular polysaccharide of Haemophilus pleuropneumoniae serotype 5. Eur J Biochem. 1987 Dec 30;170(1-2):185–192. doi: 10.1111/j.1432-1033.1987.tb13685.x. [DOI] [PubMed] [Google Scholar]
  3. Beynon L. M., Richards J. C., Perry M. B. Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 10. Carbohydr Res. 1991 Nov 11;220:185–193. doi: 10.1016/0008-6215(91)80016-g. [DOI] [PubMed] [Google Scholar]
  4. Bhattacharjee A. K., Jennings H. J., Kenny C. P. Structural elucidation of the 3-deoxy-D-manno-octulosonic acid containing meningococcal 29-e capsular polysaccharide antigen using carbon-13 nuclear magnetic resonance. Biochemistry. 1978 Feb 21;17(4):645–651. doi: 10.1021/bi00597a013. [DOI] [PubMed] [Google Scholar]
  5. Bozhko V. G., Denisov I. I., Rybkin V. S., Popov S. F., Vasil'ev V. P. Vliianie kislogo ékzopolisakharida, produtsiruemogo vozbuditelem melioidoza, na funktsional'nuiu aktivnost' makrofagov. Mikrobiol Zh. 1992 Nov-Dec;54(6):54–58. [PubMed] [Google Scholar]
  6. CHAMBON L., FOURNIER J. Constitution antigénique de Malleomyces pseudo-Mallei. I. Caractères morphologiques, culturaux, biochimiques et variations de type immunologique. Ann Inst Pasteur (Paris) 1956 Sep;91(3):355–362. [PubMed] [Google Scholar]
  7. CHAMBON L., FOURNIER J. Constitution antigénique de Malleomyces pseudo-mallei. II. Démonstration de l'existence des antigènes M, K et O et étude de leurs propriétés immunologiques. Ann Inst Pasteur (Paris) 1956 Oct;91(4):472–485. [PubMed] [Google Scholar]
  8. CHAMBON L., STAUB A. M., BOURDET M. Constitution antigénique de Malleomyces pseudomallei. III. Mode d'extraction et premiers essais de purification de l'antigène d'enveloppe K. Ann Inst Pasteur (Paris) 1957 Sep;93(3):376–389. [PubMed] [Google Scholar]
  9. Carlson R. W., Hollingsworth R. L., Dazzo F. B. A core oligosaccharide component from the lipopolysaccharide of Rhizobium trifolii ANU843. Carbohydr Res. 1988 May 1;176(1):127–135. doi: 10.1016/0008-6215(88)84064-3. [DOI] [PubMed] [Google Scholar]
  10. Cox A. D., Wilkinson S. G. Structure of the O-specific polymer for Pseudomonas cepacia serogroup O7. Carbohydr Res. 1990 Apr 2;198(1):153–156. doi: 10.1016/0008-6215(90)84286-4. [DOI] [PubMed] [Google Scholar]
  11. Dance D. A. Melioidosis: the tip of the iceberg? Clin Microbiol Rev. 1991 Jan;4(1):52–60. doi: 10.1128/cmr.4.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Denisov I. I., Iliukhin V. I., Khrapova N. P., Mel'nikov B. I., Farber S. M., Bozhko V. G., Sorokin V. Iu, Kapliev V. I. Kislyi ékzopolisakharid vozbuditelia melioidoza. Zh Mikrobiol Epidemiol Immunobiol. 1995 Nov-Dec;(6):16–17. [PubMed] [Google Scholar]
  13. Griffiss J. M., Apicella M. A., Greenwood B., Mäkelä P. H. Vaccines against encapsulated bacteria: a global agenda. Rev Infect Dis. 1987 Jan-Feb;9(1):176–188. doi: 10.1093/clinids/9.1.176. [DOI] [PubMed] [Google Scholar]
  14. HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
  15. Jann B., Jann K. Structure and biosynthesis of the capsular antigens of Escherichia coli. Curr Top Microbiol Immunol. 1990;150:19–42. doi: 10.1007/978-3-642-74694-9_2. [DOI] [PubMed] [Google Scholar]
  16. Johnson K. G., Perry M. B. Improved techniques for the preparation of bacterial lipopolysaccharides. Can J Microbiol. 1976 Jan;22(1):29–34. doi: 10.1139/m76-004. [DOI] [PubMed] [Google Scholar]
  17. Kumar A., Ernst R. R., Wüthrich K. A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules. Biochem Biophys Res Commun. 1980 Jul 16;95(1):1–6. doi: 10.1016/0006-291x(80)90695-6. [DOI] [PubMed] [Google Scholar]
  18. Lee C. J. Bacterial capsular polysaccharides--biochemistry, immunity and vaccine. Mol Immunol. 1987 Oct;24(10):1005–1019. doi: 10.1016/0161-5890(87)90067-8. [DOI] [PubMed] [Google Scholar]
  19. Leelarasamee A., Bovornkitti S. Melioidosis: review and update. Rev Infect Dis. 1989 May-Jun;11(3):413–425. doi: 10.1093/clinids/11.3.413. [DOI] [PubMed] [Google Scholar]
  20. Mort A. J., Parker S., Kuo M. S. Recovery of methylated saccharides from methylation reaction mixtures using Sep-Pak C18 cartridges. Anal Biochem. 1983 Sep;133(2):380–384. doi: 10.1016/0003-2697(83)90098-2. [DOI] [PubMed] [Google Scholar]
  21. Perry M. B., MacLean L. L., Schollaardt T., Bryan L. E., Ho M. Structural characterization of the lipopolysaccharide O antigens of Burkholderia pseudomallei. Infect Immun. 1995 Sep;63(9):3348–3352. doi: 10.1128/iai.63.9.3348-3352.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Peters T., Meyer B., Stuike-Prill R., Somorjai R., Brisson J. R. A Monte Carlo method for conformational analysis of saccharides. Carbohydr Res. 1993 Jan 15;238:49–73. doi: 10.1016/0008-6215(93)87005-d. [DOI] [PubMed] [Google Scholar]
  23. Popov S. F., Kurilov V. Ia, Iakovlev A. T. Pseudomonas pseudomallei i Pseudomonas mallei--kapsuloobrazuiushchie bakterii. Zh Mikrobiol Epidemiol Immunobiol. 1995 Sep-Oct;(5):32–36. [PubMed] [Google Scholar]
  24. Robbins J. B. Vaccines for the prevention of encapsulated bacterial diseases: current status, problems and prospects for the future. Immunochemistry. 1978 Nov;15(10-11):839–854. doi: 10.1016/0161-5890(78)90117-7. [DOI] [PubMed] [Google Scholar]
  25. Schmidt M. A., Jann K. Structure of the 2-keto-3-deoxy-D-manno-octonic-acid-containing capsular polysaccharide (K12 antigen) of the urinary-tract-infective Escherichia coli O4:K12:H-. Eur J Biochem. 1983 Apr 5;131(3):509–517. doi: 10.1111/j.1432-1033.1983.tb07291.x. [DOI] [PubMed] [Google Scholar]
  26. Steinmetz I., Rohde M., Brenneke B. Purification and characterization of an exopolysaccharide of Burkholderia (Pseudomonas) pseudomallei. Infect Immun. 1995 Oct;63(10):3959–3965. doi: 10.1128/iai.63.10.3959-3965.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Williams D. T., Perry M. B. The structure, synthesis, analysis, and occurrence of 3-deoxy-D-manno-octulosonic acid. Can J Biochem. 1969 Jul;47(7):691–695. doi: 10.1139/o69-106. [DOI] [PubMed] [Google Scholar]
  28. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T., Arakawa M. Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol. 1992;36(12):1251–1275. doi: 10.1111/j.1348-0421.1992.tb02129.x. [DOI] [PubMed] [Google Scholar]

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