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. 1997 Sep;179(18):5943–5946. doi: 10.1128/jb.179.18.5943-5946.1997

benK encodes a hydrophobic permease-like protein involved in benzoate degradation by Acinetobacter sp. strain ADP1.

L S Collier 1, N N Nichols 1, E L Neidle 1
PMCID: PMC179488  PMID: 9294456

Abstract

The chromosomal benK gene was identified within a supraoperonic gene cluster involved in benzoate degradation by Acinetobacter sp. strain ADP1, and benK was expressed in response to a benzoate metabolite, cis,cis-muconate. The disruption of benK reduced benzoate uptake and impaired the use of benzoate or benzaldehyde as the carbon source. BenK was homologous to several aromatic compound transporters.

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Selected References

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  1. Chou P. Y., Fasman G. D. Prediction of protein conformation. Biochemistry. 1974 Jan 15;13(2):222–245. doi: 10.1021/bi00699a002. [DOI] [PubMed] [Google Scholar]
  2. Conley M. P., Berg H. C., Tawa P., Stewart R. C., Ellefson D. D., Wolfe A. J. pH dependence of CheA autophosphorylation in Escherichia coli. J Bacteriol. 1994 Jul;176(13):3870–3877. doi: 10.1128/jb.176.13.3870-3877.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engelman D. M., Steitz T. A., Goldman A. Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annu Rev Biophys Biophys Chem. 1986;15:321–353. doi: 10.1146/annurev.bb.15.060186.001541. [DOI] [PubMed] [Google Scholar]
  5. Eraso J. M., Kaplan S. prrA, a putative response regulator involved in oxygen regulation of photosynthesis gene expression in Rhodobacter sphaeroides. J Bacteriol. 1994 Jan;176(1):32–43. doi: 10.1128/jb.176.1.32-43.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gralton E. M., Campbell A. L., Neidle E. L. Directed introduction of DNA cleavage sites to produce a high-resolution genetic and physical map of the Acinetobacter sp. strain ADP1 (BD413UE) chromosome. Microbiology. 1997 Apr;143(Pt 4):1345–1357. doi: 10.1099/00221287-143-4-1345. [DOI] [PubMed] [Google Scholar]
  7. Harwood C. S., Nichols N. N., Kim M. K., Ditty J. L., Parales R. E. Identification of the pcaRKF gene cluster from Pseudomonas putida: involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate. J Bacteriol. 1994 Nov;176(21):6479–6488. doi: 10.1128/jb.176.21.6479-6488.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harwood C. S., Parales R. E. The beta-ketoadipate pathway and the biology of self-identity. Annu Rev Microbiol. 1996;50:553–590. doi: 10.1146/annurev.micro.50.1.553. [DOI] [PubMed] [Google Scholar]
  9. Juni E., Janik A. Transformation of Acinetobacter calco-aceticus (Bacterium anitratum). J Bacteriol. 1969 Apr;98(1):281–288. doi: 10.1128/jb.98.1.281-288.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keen N. T., Tamaki S., Kobayashi D., Trollinger D. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene. 1988 Oct 15;70(1):191–197. doi: 10.1016/0378-1119(88)90117-5. [DOI] [PubMed] [Google Scholar]
  11. Kihara M., Macnab R. M. Cytoplasmic pH mediates pH taxis and weak-acid repellent taxis of bacteria. J Bacteriol. 1981 Mar;145(3):1209–1221. doi: 10.1128/jb.145.3.1209-1221.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kokotek W., Lotz W. Construction of a lacZ-kanamycin-resistance cassette, useful for site-directed mutagenesis and as a promoter probe. Gene. 1989 Dec 14;84(2):467–471. doi: 10.1016/0378-1119(89)90522-2. [DOI] [PubMed] [Google Scholar]
  13. Kowalchuk G. A., Hartnett G. B., Benson A., Houghton J. E., Ngai K. L., Ornston L. N. Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon. Gene. 1994 Aug 19;146(1):23–30. doi: 10.1016/0378-1119(94)90829-x. [DOI] [PubMed] [Google Scholar]
  14. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  15. Leveau J. H., van der Meer J. R. The tfdR gene product can successfully take over the role of the insertion element-inactivated TfdT protein as a transcriptional activator of the tfdCDEF gene cluster, which encodes chlorocatechol degradation in Ralstonia eutropha JMP134(pJP4) J Bacteriol. 1996 Dec;178(23):6824–6832. doi: 10.1128/jb.178.23.6824-6832.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marger M. D., Saier M. H., Jr A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport. Trends Biochem Sci. 1993 Jan;18(1):13–20. doi: 10.1016/0968-0004(93)90081-w. [DOI] [PubMed] [Google Scholar]
  17. Merkel S. M., Eberhard A. E., Gibson J., Harwood C. S. Involvement of coenzyme A thioesters in anaerobic metabolism of 4-hydroxybenzoate by Rhodopseudomonas palustris. J Bacteriol. 1989 Jan;171(1):1–7. doi: 10.1128/jb.171.1.1-7.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Neidle E. L., Hartnett C., Ornston L. N., Bairoch A., Rekik M., Harayama S. Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases. J Bacteriol. 1991 Sep;173(17):5385–5395. doi: 10.1128/jb.173.17.5385-5395.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Neidle E. L., Hartnett C., Ornston L. N. Characterization of Acinetobacter calcoaceticus catM, a repressor gene homologous in sequence to transcriptional activator genes. J Bacteriol. 1989 Oct;171(10):5410–5421. doi: 10.1128/jb.171.10.5410-5421.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nichols N. N., Harwood C. S. PcaK, a high-affinity permease for the aromatic compounds 4-hydroxybenzoate and protocatechuate from Pseudomonas putida. J Bacteriol. 1997 Aug;179(16):5056–5061. doi: 10.1128/jb.179.16.5056-5061.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Perrotta J. A., Harwood C. S. Anaerobic Metabolism of Cyclohex-1-Ene-1-Carboxylate, a Proposed Intermediate of Benzoate Degradation, by Rhodopseudomonas palustris. Appl Environ Microbiol. 1994 Jun;60(6):1775–1782. doi: 10.1128/aem.60.6.1775-1782.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Prentki P., Krisch H. M. In vitro insertional mutagenesis with a selectable DNA fragment. Gene. 1984 Sep;29(3):303–313. doi: 10.1016/0378-1119(84)90059-3. [DOI] [PubMed] [Google Scholar]
  23. Romero-Arroyo C. E., Schell M. A., Gaines G. L., 3rd, Neidle E. L. catM encodes a LysR-type transcriptional activator regulating catechol degradation in Acinetobacter calcoaceticus. J Bacteriol. 1995 Oct;177(20):5891–5898. doi: 10.1128/jb.177.20.5891-5898.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shanley M. S., Neidle E. L., Parales R. E., Ornston L. N. Cloning and expression of Acinetobacter calcoaceticus catBCDE genes in Pseudomonas putida and Escherichia coli. J Bacteriol. 1986 Feb;165(2):557–563. doi: 10.1128/jb.165.2.557-563.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Williams P. A., Shaw L. E. mucK, a gene in Acinetobacter calcoaceticus ADP1 (BD413), encodes the ability to grow on exogenous cis,cis-muconate as the sole carbon source. J Bacteriol. 1997 Sep;179(18):5935–5942. doi: 10.1128/jb.179.18.5935-5942.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]

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