Abstract
Application of a fluorescence technique for the detection of the human Y chromosome in interphase nuclei indicates that the best results may be obtained from the study of lymphocytes in blood smears. The most satisfactory Y-fluorescence is obtained with the use of buffered quinacrine mustard dihydrochloride. The method can be complemented by other standard techniques to obtain a clear idea of the sex-chromosome complement of the individual. The application of this technique to clinical and chromosomal diagnosis and to the prenatal detection of the Y chromosome in amniotic cells seems at least as promising as has proved the earlier identification of the Barr body.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barlow P., Vosa C. G. The Y chromosome in human spermatozoa. Nature. 1970 Jun 6;226(5249):961–962. doi: 10.1038/226961a0. [DOI] [PubMed] [Google Scholar]
- Caspersson T., Farber S., Foley G. E., Kudynowski J., Modest E. J., Simonsson E., Wagh U., Zech L. Chemical differentiation along metaphase chromosomes. Exp Cell Res. 1968 Jan;49(1):219–222. doi: 10.1016/0014-4827(68)90538-7. [DOI] [PubMed] [Google Scholar]
- Caspersson T., Zech L., Johansson C. Differential binding of alkylating fluorochromes in human chromosomes. Exp Cell Res. 1970 Jun;60(3):315–319. doi: 10.1016/0014-4827(70)90523-9. [DOI] [PubMed] [Google Scholar]
- Caspersson T., Zech L., Johansson C., Modest E. J. Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma. 1970;30(2):215–227. doi: 10.1007/BF00282002. [DOI] [PubMed] [Google Scholar]
- George K. P. Cytochemical differentiation along human chromosomes. Nature. 1970 Apr 4;226(5240):80–81. doi: 10.1038/226080a0. [DOI] [PubMed] [Google Scholar]
- Loveless A. Ambiguous mustard. Nature. 1970 Jul 4;227(5253):101–101. doi: 10.1038/227101c0. [DOI] [PubMed] [Google Scholar]
- Pearson P. L., Bobrow M. Definitive evidence for the short arm of the Y chromosome associating with the X chromosome during miosis in the human male. Nature. 1970 Jun 6;226(5249):959–961. doi: 10.1038/226959a0. [DOI] [PubMed] [Google Scholar]
- Pearson P. L., Bobrow M., Vosa C. G. Technique for identifying Y chromosomes in human interphase nuclei. Nature. 1970 Apr 4;226(5240):78–80. doi: 10.1038/226078a0. [DOI] [PubMed] [Google Scholar]