Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1997 Oct;179(20):6504–6508. doi: 10.1128/jb.179.20.6504-6508.1997

Evidence that rseC, a gene in the rpoE cluster, has a role in thiamine synthesis in Salmonella typhimurium.

B J Beck 1, L E Connolly 1, A De Las Peñas 1, D M Downs 1
PMCID: PMC179570  PMID: 9335303

Abstract

In Salmonella typhimurium, the genetic loci and biochemical reactions necessary for the conversion of aminoimidazole ribotide (AIR) to the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiamine remain unknown. Preliminary genetic analysis indicates that there may be more than one pathway responsible for the synthesis of HMP from AIR and that the function of these pathways depends on the availability of AIR, synthesized by the purine pathway or by the purF-independent alternative pyrimidine biosynthetic (APB) pathway (L. Petersen and D. Downs, J. Bacteriol. 178:5676-5682, 1996). An insertion in rseB, the third gene in the rpoE rseABC gene cluster at 57 min, prevented HMP synthesis in a purF mutant. Complementation analysis demonstrated that the HMP requirement of the purF rseB strain was due to polarity of the insertion in rseB on the downstream rseC gene. The role of RseC in thiamine synthesis was independent of rpoE.

Full Text

The Full Text of this article is available as a PDF (638.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Benson N. R., Goldman B. S. Rapid mapping in Salmonella typhimurium with Mud-P22 prophages. J Bacteriol. 1992 Mar;174(5):1673–1681. doi: 10.1128/jb.174.5.1673-1681.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chi E., Bartlett D. H. An rpoE-like locus controls outer membrane protein synthesis and growth at cold temperatures and high pressures in the deep-sea bacterium Photobacterium sp. strain SS9. Mol Microbiol. 1995 Aug;17(4):713–726. doi: 10.1111/j.1365-2958.1995.mmi_17040713.x. [DOI] [PubMed] [Google Scholar]
  4. De Las Peñas A., Connolly L., Gross C. A. The sigmaE-mediated response to extracytoplasmic stress in Escherichia coli is transduced by RseA and RseB, two negative regulators of sigmaE. Mol Microbiol. 1997 Apr;24(2):373–385. doi: 10.1046/j.1365-2958.1997.3611718.x. [DOI] [PubMed] [Google Scholar]
  5. Downs D. M. Evidence for a new, oxygen-regulated biosynthetic pathway for the pyrimidine moiety of thiamine in Salmonella typhimurium. J Bacteriol. 1992 Mar;174(5):1515–1521. doi: 10.1128/jb.174.5.1515-1521.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Enos-Berlage J. L., Downs D. M. Involvement of the oxidative pentose phosphate pathway in thiamine biosynthesis in Salmonella typhimurium. J Bacteriol. 1996 Mar;178(5):1476–1479. doi: 10.1128/jb.178.5.1476-1479.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fleischmann R. D., Adams M. D., White O., Clayton R. A., Kirkness E. F., Kerlavage A. R., Bult C. J., Tomb J. F., Dougherty B. A., Merrick J. M. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. 1995 Jul 28;269(5223):496–512. doi: 10.1126/science.7542800. [DOI] [PubMed] [Google Scholar]
  8. Gish W., States D. J. Identification of protein coding regions by database similarity search. Nat Genet. 1993 Mar;3(3):266–272. doi: 10.1038/ng0393-266. [DOI] [PubMed] [Google Scholar]
  9. Lonetto M. A., Brown K. L., Rudd K. E., Buttner M. J. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulation of extracytoplasmic functions. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7573–7577. doi: 10.1073/pnas.91.16.7573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martínez-Salazar J. M., Moreno S., Nájera R., Boucher J. C., Espín G., Soberón-Chávez G., Deretic V. Characterization of the genes coding for the putative sigma factor AlgU and its regulators MucA, MucB, MucC, and MucD in Azotobacter vinelandii and evaluation of their roles in alginate biosynthesis. J Bacteriol. 1996 Apr;178(7):1800–1808. doi: 10.1128/jb.178.7.1800-1808.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Missiakas D., Mayer M. P., Lemaire M., Georgopoulos C., Raina S. Modulation of the Escherichia coli sigmaE (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins. Mol Microbiol. 1997 Apr;24(2):355–371. doi: 10.1046/j.1365-2958.1997.3601713.x. [DOI] [PubMed] [Google Scholar]
  12. Newell P. C., Tucker R. G. New pyrimidine pathway involved in the biosynthesis of the pyrimidine of thiamine. Nature. 1967 Sep 23;215(5108):1384–1385. doi: 10.1038/2151384a0. [DOI] [PubMed] [Google Scholar]
  13. O'Brien K., Deno G., Ostrovsky de Spicer P., Gardner J. F., Maloy S. R. Integration host factor facilitates repression of the put operon in Salmonella typhimurium. Gene. 1992 Sep 1;118(1):13–19. doi: 10.1016/0378-1119(92)90243-i. [DOI] [PubMed] [Google Scholar]
  14. Petersen L., Downs D. M. Mutations in apbC (mrp) prevent function of the alternative pyrimidine biosynthetic pathway in Salmonella typhimurium. J Bacteriol. 1996 Oct;178(19):5676–5682. doi: 10.1128/jb.178.19.5676-5682.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Petersen L., Enos-Berlage J., Downs D. M. Genetic analysis of metabolic crosstalk and its impact on thiamine synthesis in Salmonella typhimurium. Genetics. 1996 May;143(1):37–44. doi: 10.1093/genetics/143.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schmehl M., Jahn A., Meyer zu Vilsendorf A., Hennecke S., Masepohl B., Schuppler M., Marxer M., Oelze J., Klipp W. Identification of a new class of nitrogen fixation genes in Rhodobacter capsulatus: a putative membrane complex involved in electron transport to nitrogenase. Mol Gen Genet. 1993 Dec;241(5-6):602–615. doi: 10.1007/BF00279903. [DOI] [PubMed] [Google Scholar]
  17. Schurr M. J., Yu H., Martinez-Salazar J. M., Boucher J. C., Deretic V. Control of AlgU, a member of the sigma E-like family of stress sigma factors, by the negative regulators MucA and MucB and Pseudomonas aeruginosa conversion to mucoidy in cystic fibrosis. J Bacteriol. 1996 Aug;178(16):4997–5004. doi: 10.1128/jb.178.16.4997-5004.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Webb E., Downs D. Characterization of thiL, encoding thiamin-monophosphate kinase, in Salmonella typhimurium. J Biol Chem. 1997 Jun 20;272(25):15702–15707. doi: 10.1074/jbc.272.25.15702. [DOI] [PubMed] [Google Scholar]
  19. Xie Z. D., Hershberger C. D., Shankar S., Ye R. W., Chakrabarty A. M. Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA. J Bacteriol. 1996 Aug;178(16):4990–4996. doi: 10.1128/jb.178.16.4990-4996.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES