Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1997 Nov;179(21):6865–6870. doi: 10.1128/jb.179.21.6865-6870.1997

cis-Acting sequences required for light-responsive expression of the psbDII gene in Synechococcus sp. strain PCC 7942.

S Anandan 1, S S Golden 1
PMCID: PMC179622  PMID: 9352943

Abstract

We analyzed the sequences required for promoter activity and high-light responsiveness of the psbDII gene in the cyanobacterium Synechococcus sp. strain PCC 7942 by using transcriptional fusions to a lacZ reporter gene. The basal promoter drives high constitutive expression, although no canonical -35 element is evident. The smallest fragment that showed clear light-responsive expression extends from -38 to +160, which includes 52 bp of the psbDII open reading frame. Sequences downstream from the promoter, within the untranslated leader region from +11 to +24, were required for high-light induction.

Full Text

The Full Text of this article is available as a PDF (775.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bustos S. A., Golden S. S. Expression of the psbDII gene in Synechococcus sp. strain PCC 7942 requires sequences downstream of the transcription start site. J Bacteriol. 1991 Dec;173(23):7525–7533. doi: 10.1128/jb.173.23.7525-7533.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bustos S. A., Golden S. S. Light-regulated expression of the psbD gene family in Synechococcus sp. strain PCC 7942: evidence for the role of duplicated psbD genes in cyanobacteria. Mol Gen Genet. 1992 Mar;232(2):221–230. doi: 10.1007/BF00280000. [DOI] [PubMed] [Google Scholar]
  3. Elledge S. J., Sugiono P., Guarente L., Davis R. W. Genetic selection for genes encoding sequence-specific DNA-binding proteins. Proc Natl Acad Sci U S A. 1989 May;86(10):3689–3693. doi: 10.1073/pnas.86.10.3689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Golden S. S., Brusslan J., Haselkorn R. Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2. EMBO J. 1986 Nov;5(11):2789–2798. doi: 10.1002/j.1460-2075.1986.tb04569.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Golden S. S., Brusslan J., Haselkorn R. Genetic engineering of the cyanobacterial chromosome. Methods Enzymol. 1987;153:215–231. doi: 10.1016/0076-6879(87)53055-5. [DOI] [PubMed] [Google Scholar]
  6. Golden S. S., Cho D. S., Nalty M. S. Two functional psbD genes in the cyanobacterium Synechococcus sp. strain PCC 7942. J Bacteriol. 1989 Sep;171(9):4707–4713. doi: 10.1128/jb.171.9.4707-4713.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Golden S. S., Stearns G. W. Nucleotide sequence and transcript analysis of three photosystem II genes from the cyanobacterium Synechococcus sp. PCC7942. Gene. 1988 Jul 15;67(1):85–96. doi: 10.1016/0378-1119(88)90011-x. [DOI] [PubMed] [Google Scholar]
  8. Higuchi R., Krummel B., Saiki R. K. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 1988 Aug 11;16(15):7351–7367. doi: 10.1093/nar/16.15.7351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Horton R. M., Hunt H. D., Ho S. N., Pullen J. K., Pease L. R. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene. 1989 Apr 15;77(1):61–68. doi: 10.1016/0378-1119(89)90359-4. [DOI] [PubMed] [Google Scholar]
  10. Kulkarni R. D., Golden S. S. mRNA stability is regulated by a coding-region element and the unique 5' untranslated leader sequences of the three Synechococcus psbA transcripts. Mol Microbiol. 1997 Jun;24(6):1131–1142. doi: 10.1046/j.1365-2958.1997.4201768.x. [DOI] [PubMed] [Google Scholar]
  11. Kumar A., Malloch R. A., Fujita N., Smillie D. A., Ishihama A., Hayward R. S. The minus 35-recognition region of Escherichia coli sigma 70 is inessential for initiation of transcription at an "extended minus 10" promoter. J Mol Biol. 1993 Jul 20;232(2):406–418. doi: 10.1006/jmbi.1993.1400. [DOI] [PubMed] [Google Scholar]
  12. Kustu S., Santero E., Keener J., Popham D., Weiss D. Expression of sigma 54 (ntrA)-dependent genes is probably united by a common mechanism. Microbiol Rev. 1989 Sep;53(3):367–376. doi: 10.1128/mr.53.3.367-376.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Li R., Golden S. S. Enhancer activity of light-responsive regulatory elements in the untranslated leader regions of cyanobacterial psbA genes. Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11678–11682. doi: 10.1073/pnas.90.24.11678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McClure W. R. Mechanism and control of transcription initiation in prokaryotes. Annu Rev Biochem. 1985;54:171–204. doi: 10.1146/annurev.bi.54.070185.001131. [DOI] [PubMed] [Google Scholar]
  15. Reznikoff W. S., Siegele D. A., Cowing D. W., Gross C. A. The regulation of transcription initiation in bacteria. Annu Rev Genet. 1985;19:355–387. doi: 10.1146/annurev.ge.19.120185.002035. [DOI] [PubMed] [Google Scholar]
  16. Tanaka K., Masuda S., Takahashi H. Multiple rpoD-related genes of cyanobacteria. Biosci Biotechnol Biochem. 1992 Jul;56(7):1113–1117. doi: 10.1271/bbb.56.1113. [DOI] [PubMed] [Google Scholar]
  17. Tanaka K., Masuda S., Takahashi H. The complete nucleotide sequence of the gene (rpoD1) encoding the principal sigma factor of the RNA polymerase from the cyanobacterium Synechococcus sp. strain PCC7942. Biochim Biophys Acta. 1992 Aug 17;1132(1):94–96. doi: 10.1016/0167-4781(92)90060-d. [DOI] [PubMed] [Google Scholar]
  18. Tsinoremas N. F., Ishiura M., Kondo T., Andersson C. R., Tanaka K., Takahashi H., Johnson C. H., Golden S. S. A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria. EMBO J. 1996 May 15;15(10):2488–2495. [PMC free article] [PubMed] [Google Scholar]
  19. Wei T. F., Ramasubramanian T. S., Pu F., Golden J. W. Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection. J Bacteriol. 1993 Jul;175(13):4025–4035. doi: 10.1128/jb.175.13.4025-4035.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yarchuk O., Jacques N., Guillerez J., Dreyfus M. Interdependence of translation, transcription and mRNA degradation in the lacZ gene. J Mol Biol. 1992 Aug 5;226(3):581–596. doi: 10.1016/0022-2836(92)90617-s. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES