Abstract
This is a correction of an earlier published article.
Text
After the publication of this work[1], we became aware that the our description of the methods used to identify Shigella spp. did not describe the identification of a subset of Shigella spp. which were untypeable with commercial sera. Additional typing was conducted by Dr. Kaisar A. Talukder and coworkers (Laboratory Sciences Division, ICDDR, B: Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh. Email:kaisar@icddrb.org.)
Brief description of the methods used for serotyping the untypeable S. flexneri strains using MASF
All Shigella strains which agglutinated with commercial polyvalent anti-S. flexneri serum (Denka Seiken, Tokyo, Japan) but were untypeable using commercial serotype specific antisera were examined using a panel of monoclonal antibodies against S. flexneri (MASF B) according to the typing scheme shown in Table 1 using monoclonal antibodies from one supplier (Reagensia AB, Stockholm, Sweden) with the exception of MASF 1c which was provided by Dr. N. I. A. Carlin (SBL, Sweden) [2-5]. Untypeable S. flexneri strains which agglutinated strongly with polyclonal MASF B and type antigen factor IV-2 but not with group factor Y-5 or 6 thereby excluding their characterization into serotype 4a or 4b were designated as serotype 4 if they agglutinated with a new antigenic determinant E1037 (IV-1). Untypeable strains which agglutinated with antibodies against the provisional antigen, MASF IV-1 specific for the new antigenic determinant E1037, were designated as 4X. Untypeable strains which agglutinated only with MASF Ic were designated as 1c.
Table 1.
S. flexneri serotyping criteria using MASF antibodies
| Serotype | Type antigen specific | MASF B | Group antigen specific | Provisional antigen specific | |||||||
| 1 (1:-)a | 11 (11:-) | IV-2 (IV:-) | V (V:-) | VI (VI:-) | B (-:1) | Y-5 (-:3.4) | 6 (-:6) | 7.8 (-:7.8) | IV-1 (-:E1037) | 1c | |
| 1a | + | + | + | ||||||||
| 1b | + | + | + | ||||||||
| 1c | + | + | |||||||||
| 2a | + | + | + | ||||||||
| 2b | + | + | + | ||||||||
| 3a | + | + | + | ||||||||
| 3b | + | + | + | ||||||||
| 3c | + | + | |||||||||
| 4:- | + | + | + | ||||||||
| 4a | + | + | + | +/- | |||||||
| 4b | + | + | + | ||||||||
| 5a | + | + | + | ||||||||
| 5b | + | + | + | ||||||||
| 6 | + | + | +/- | ||||||||
| X | + | + | +/- | ||||||||
| Y | + | + | +/- | ||||||||
| 4X | + | + | |||||||||
a Shown in parentheses is the antigen recognized.
+ Positive reaction
Pre-publication history
The pre-publication history for this paper can be accessed here:
Contributor Information
Magdarina D Agtini, Email: magdarina@yahoo.com.
Rooswanti Soeharno, Email: rooswanti@litbang.depkes.go.id.
Murad Lesmana, Email: murad@namru2.org.
Narain H Punjabi, Email: narain@namru2.org.
Cyrus Simanjuntak, Email: cyrushs@hotmail.com.
Ferry Wangsasaputra, Email: ferry@namru2.med.navy.mil.
Dazwir Nurdin, Email: ferry@namru2.med.navy.mil.
Sri Pandam Pulungsih, Email: rspiss@idola.net.id.
Ainur Rofiq, Email: rofiq@idola.net.id.
Hari Santoso, Email: santoso@hotmail.com.
H Pujarwoto, Email: ferry@hotmail.com.
Agus Sjahrurachman, Email: Sjahrurachman@idola.net.id.
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References
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