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. 1997 Dec;179(24):7812–7815. doi: 10.1128/jb.179.24.7812-7815.1997

Synthesis of nitric oxide from the two equivalent guanidino nitrogens of L-arginine by Lactobacillus fermentum.

H Morita 1, H Yoshikawa 1, R Sakata 1, Y Nagata 1, H Tanaka 1
PMCID: PMC179746  PMID: 9401042

Abstract

Ten strains of Lactobacillus fermentum that differed in origin converted metmyoglobin to nitrosylmyoglobin [a pentacoordinate nitric oxide (NO) complex of Fe(II) myoglobin] in MRS broth at pH 4.3. Of the 10 strains, L. fermentum IFO 3956 possessed the strongest capacity to convert metmyoglobin to nitrosylmyoglobin. This strain synthesizes NO enzymatically from the two equivalent guanidino nitrogens of L-arginine. To our knowledge, this demonstrates for the first time the production of NO synthesized from the guanidino nitrogens of L-arginine by lactic acid bacteria. IFO 3956 may possess a bacterial NO synthase.

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Selected References

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  1. Chen Y., Rosazza J. P. A bacterial nitric oxide synthase from a Nocardia species. Biochem Biophys Res Commun. 1994 Sep 15;203(2):1251–1258. doi: 10.1006/bbrc.1994.2317. [DOI] [PubMed] [Google Scholar]
  2. Chen Y., Rosazza J. P. Purification and characterization of nitric oxide synthase (NOSNoc) from a Nocardia species. J Bacteriol. 1995 Sep;177(17):5122–5128. doi: 10.1128/jb.177.17.5122-5128.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cunin R., Glansdorff N., Piérard A., Stalon V. Biosynthesis and metabolism of arginine in bacteria. Microbiol Rev. 1986 Sep;50(3):314–352. doi: 10.1128/mr.50.3.314-352.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Duprat A. F., Traylor T. G., Wu G. Z., Coletta M., Sharma V. S., Walda K. N., Magde D. Myoglobin-NO at low pH: free four-coordinated heme in the protein pocket. Biochemistry. 1995 Feb 28;34(8):2634–2644. doi: 10.1021/bi00008a030. [DOI] [PubMed] [Google Scholar]
  5. Goretski J., Hollocher T. C. The kinetic and isotopic competence of nitric oxide as an intermediate in denitrification. J Biol Chem. 1990 Jan 15;265(2):889–895. [PubMed] [Google Scholar]
  6. Green L. C., Wagner D. A., Glogowski J., Skipper P. L., Wishnok J. S., Tannenbaum S. R. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem. 1982 Oct;126(1):131–138. doi: 10.1016/0003-2697(82)90118-x. [DOI] [PubMed] [Google Scholar]
  7. Hevel J. M., White K. A., Marletta M. A. Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein. J Biol Chem. 1991 Dec 5;266(34):22789–22791. [PubMed] [Google Scholar]
  8. Iyengar R., Stuehr D. J., Marletta M. A. Macrophage synthesis of nitrite, nitrate, and N-nitrosamines: precursors and role of the respiratory burst. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6369–6373. doi: 10.1073/pnas.84.18.6369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 1992 Sep;6(12):3051–3064. [PubMed] [Google Scholar]
  11. Papen H., von Berg R., Hinkel I., Thoene B., Rennenberg H. Heterotrophic nitrification by Alcaligenes faecalis: NO2-, NO3-, N2O, and NO production in exponentially growing cultures. Appl Environ Microbiol. 1989 Aug;55(8):2068–2072. doi: 10.1128/aem.55.8.2068-2072.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pollock J. S., Förstermann U., Mitchell J. A., Warner T. D., Schmidt H. H., Nakane M., Murad F. Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10480–10484. doi: 10.1073/pnas.88.23.10480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Poolman B., Driessen A. J., Konings W. N. Regulation of arginine-ornithine exchange and the arginine deiminase pathway in Streptococcus lactis. J Bacteriol. 1987 Dec;169(12):5597–5604. doi: 10.1128/jb.169.12.5597-5604.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stuehr D. J., Cho H. J., Kwon N. S., Weise M. F., Nathan C. F. Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an FAD- and FMN-containing flavoprotein. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7773–7777. doi: 10.1073/pnas.88.17.7773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stuehr D. J., Griffith O. W. Mammalian nitric oxide synthases. Adv Enzymol Relat Areas Mol Biol. 1992;65:287–346. doi: 10.1002/9780470123119.ch8. [DOI] [PubMed] [Google Scholar]
  16. Tesch J. W., Rehg W. R., Sievers R. E. Microdetermination of nitrates and nitrites in saliva, blood, water, and suspended particulates in air by gas chromatography. J Chromatogr. 1976 Nov 3;126:743–755. doi: 10.1016/s0021-9673(01)84117-0. [DOI] [PubMed] [Google Scholar]
  17. Vane J. R., Botting R. M. Secretory functions of the vascular endothelium. J Physiol Pharmacol. 1992 Sep;43(3):195–207. [PubMed] [Google Scholar]
  18. Ye R. W., Averill B. A., Tiedje J. M. Denitrification: production and consumption of nitric oxide. Appl Environ Microbiol. 1994 Apr;60(4):1053–1058. doi: 10.1128/aem.60.4.1053-1058.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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