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. 1984 Dec;26(6):837–840. doi: 10.1128/aac.26.6.837

Enhancement of the in vitro activity of amphotericin B against Aspergillus spp. by tetracycline analogs.

C E Hughes, C Harris, L R Peterson, D N Gerding
PMCID: PMC180035  PMID: 6524900

Abstract

Strains of Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger were tested for in vitro susceptibility to amphotericin B alone and in combination with fixed concentrations of tetracycline, doxycycline, or minocycline, using buffered minimal essential medium in microtiter plates. Enhanced inhibitory activity was seen, especially with combinations of amphotericin B and minocycline. Synergistic activity between amphotericin B and minocycline was observed in each of five isolates of each species when tested in a checkerboard dilution scheme. Time-kill curves demonstrated killing an A. fumigatus isolated at concentrations of amphotericin B that were four- or eightfold lower in the presence of 5 or 15 micrograms of minocycline per ml than with amphotericin B alone. Of the tetracycline analogs tested, minocycline has the greatest activity against A. fumigatus, A. flavus, and A. niger conidia when potentiated by amphotericin B.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anséhn S., Granström S., Höjer H., Nilsson L., Akesson E., Lundin A., Thore A. In-vitro effects of Candida albicans of amphotericin B combined with other antibiotics. Preliminary observations. Scand J Infect Dis Suppl. 1976;(9):62–66. [PubMed] [Google Scholar]
  2. Arroyo J., Medoff G., Kobayashi G. S. Therapy of murine aspergillosis with amphotericin B in combination with rifampin of 5-fluorocytosine. Antimicrob Agents Chemother. 1977 Jan;11(1):21–25. doi: 10.1128/aac.11.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Battaner E., Vazquez D. Inhibitors of protein synthesis by ribosomes of the 80-S type. Biochim Biophys Acta. 1971 Dec 16;254(2):316–330. doi: 10.1016/0005-2787(71)90840-9. [DOI] [PubMed] [Google Scholar]
  4. Forsgren A., Schmeling D., Quie P. G. Effect of tetracycline on the phagocytic function of human leukocytes. J Infect Dis. 1974 Oct;130(4):412–415. doi: 10.1093/infdis/130.4.412. [DOI] [PubMed] [Google Scholar]
  5. Glette J., Sandberg S., Hopen G., Solberg C. O. Influence of tetracyclines on human polymorphonuclear leukocyte function. Antimicrob Agents Chemother. 1984 Mar;25(3):354–357. doi: 10.1128/aac.25.3.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graybill J. R., Mitchell L. Treatment of murine cryptococcosis with minocycline and amphotericin B. Sabouraudia. 1980 Jun;18(2):137–144. doi: 10.1080/00362178085380221. [DOI] [PubMed] [Google Scholar]
  7. Hallander H. O., Dornbusch K., Gezelius L., Jacobson K., Karlsson I. Synergism between aminoglycosides and cephalosporins with antipseudomonal activity: interaction index and killing curve method. Antimicrob Agents Chemother. 1982 Nov;22(5):743–752. doi: 10.1128/aac.22.5.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hughes C. E., Harris C., Moody J. A., Peterson L. R., Gerding D. N. In vitro activities of amphotericin B in combination with four antifungal agents and rifampin against Aspergillus spp. Antimicrob Agents Chemother. 1984 May;25(5):560–562. doi: 10.1128/aac.25.5.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huppert M., Sun S. H., Vukovich K. R. Combined amphotericin B-tetracycline therapy for experimental coccidioidomycosis. Antimicrob Agents Chemother. 1974 May;5(5):473–478. doi: 10.1128/aac.5.5.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kinsky S. C. Antibiotic interaction with model membranes. Annu Rev Pharmacol. 1970;10:119–142. doi: 10.1146/annurev.pa.10.040170.001003. [DOI] [PubMed] [Google Scholar]
  11. Kitahara M., Seth V. K., Medoff G., Kobayashi G. S. Activity of amphotericin B, 5-fluorocytosine, and rifampin against six clinical isolates of Aspergillus. Antimicrob Agents Chemother. 1976 Jun;9(6):915–919. doi: 10.1128/aac.9.6.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kwan C. N., Medoff G., Kobayashi G. S., Schlessinger D., Raskas H. J. Potentiation of the antifungal effects of antibiotics by amphotericin B. Antimicrob Agents Chemother. 1972 Aug;2(2):61–65. doi: 10.1128/aac.2.2.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lew M. A., Beckett K. M., Levin M. J. Antifungal activity of four tetracycline analogues against Candida albicans in vitro: potentiation by amphotericin B. J Infect Dis. 1977 Aug;136(2):263–270. doi: 10.1093/infdis/136.2.263. [DOI] [PubMed] [Google Scholar]
  14. Medoff G., Brajtburg J., Kobayashi G. S., Bolard J. Antifungal agents useful in therapy of systemic fungal infections. Annu Rev Pharmacol Toxicol. 1983;23:303–330. doi: 10.1146/annurev.pa.23.040183.001511. [DOI] [PubMed] [Google Scholar]
  15. Medoff G., Kobayashi G. S., Kwan C. N., Schlessinger D., Venkov P. Potentiation of rifampicin and 5-fluorocytosine as antifungal antibiotics by amphotericin B (yeast-membrane permeability-ribosomal RNA-eukaryotic cell-synergism). Proc Natl Acad Sci U S A. 1972 Jan;69(1):196–199. doi: 10.1073/pnas.69.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thong Y. H., Rowan-Kelly B., Shepherd C., Ferrante A. Synergism between tetracycline and amphotericin B in experimental amoebic meningoencephalitis. Med J Aust. 1978 Jun 17;1(12):663–664. doi: 10.5694/j.1326-5377.1978.tb112633.x. [DOI] [PubMed] [Google Scholar]

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