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. 1985 May;27(5):712–714. doi: 10.1128/aac.27.5.712

Effects of aminoglycoside antibiotics on polymorphonuclear leukocyte function in vivo.

F R Venezio, C A DiVincenzo
PMCID: PMC180138  PMID: 3893317

Abstract

In vitro incubation of aminoglycoside antibiotics with human polymorphonuclear leukocytes (PMNs) has been shown to induce abnormalities in cell function. This study was designed to determine whether there are similar abnormalities in leukocyte function after exposure to the action of these agents in vivo. Four aminoglycosides (gentamicin, tobramycin, netilmicin, and amikacin) were tested. In vitro incubation did not induce a chemotactic defect when measured by an under-agarose method. However, inhibition of candidacidal activity was reproducible after in vitro incubation of all aminoglycosides tested. Nevertheless, when the aminoglycosides were administered intravenously to normal volunteers, PMN function, including adherence to nylon wool columns, chemotaxis, phagocytosis, and killing of Candida albicans, was unimpaired at 1, 3, and 24 h postinfusion. Therefore, we conclude that aminoglycoside antibiotic administration does not induce PMN dysfunction in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaperon E. A., Sanders W. E., Jr Suppression of lymphocyte responses by cephalosporins. Infect Immun. 1978 Feb;19(2):378–384. doi: 10.1128/iai.19.2.378-384.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ferrari F. A., Pagani A., Marconi M., Stefanoni R., Siccardi A. G. Inhibition of candidacidal activity of human neutrophil leukocytes by aminoglycoside antibiotics. Antimicrob Agents Chemother. 1980 Jan;17(1):87–88. doi: 10.1128/aac.17.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forsgren A., Schmeling D. Effect of antibiotics of chemotaxis of human leukocytes. Antimicrob Agents Chemother. 1977 Apr;11(4):580–584. doi: 10.1128/aac.11.4.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goodhart G. L. Effect of aminoglycosides on the chemotactic response of human polymorphonuclear leukocytes. Antimicrob Agents Chemother. 1977 Oct;12(4):540–542. doi: 10.1128/aac.12.4.540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hjorth R., Jonsson A. K., Vretblad P. A rapid method for purification of human granulocytes using percoll. A comparison with dextran sedimentation. J Immunol Methods. 1981;43(1):95–101. doi: 10.1016/0022-1759(81)90040-5. [DOI] [PubMed] [Google Scholar]
  6. Lehrer R. I., Cline M. J. Interaction of Candida albicans with human leukocytes and serum. J Bacteriol. 1969 Jun;98(3):996–1004. doi: 10.1128/jb.98.3.996-1004.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lehrer R. I. Effects of colchicine and chloramphenicol on the oxidative metabolism and phagocytic activity of human neutrophils. J Infect Dis. 1973 Jan;127(1):40–48. doi: 10.1093/infdis/127.1.40. [DOI] [PubMed] [Google Scholar]
  8. MacGregor R. R., Spagnuolo P. J., Lentnek A. L. Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system. N Engl J Med. 1974 Sep 26;291(13):642–646. doi: 10.1056/NEJM197409262911302. [DOI] [PubMed] [Google Scholar]
  9. Mukerjee P., Schuldt S., Kasik J. E. Effect of rifampin on cutaneous hypersensitivity to purified protein derivative in humans. Antimicrob Agents Chemother. 1973 Dec;4(6):607–611. doi: 10.1128/aac.4.6.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Munster A. M., Loadholdt C. B., Leary A. G., Barnes M. A. The effect of antibiotics on cell-mediated immunity. Surgery. 1977 Jun;81(6):692–695. [PubMed] [Google Scholar]
  11. Nelson R. D., Quie P. G., Simmons R. L. Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J Immunol. 1975 Dec;115(6):1650–1656. [PubMed] [Google Scholar]
  12. Seklecki M. M., Quintiliani R., Maderazo E. G. Aminoglycoside antibiotics moderately impair granulocyte function. Antimicrob Agents Chemother. 1978 Mar;13(3):552–554. doi: 10.1128/aac.13.3.552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Siegel J. P., Remington J. S. Effect of antimicrobial agents on chemiluminescence of human polymorphonuclear leukocytes in response to phagocytosis. J Antimicrob Chemother. 1982 Dec;10(6):505–515. doi: 10.1093/jac/10.6.505. [DOI] [PubMed] [Google Scholar]

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