Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1985 Jun;27(6):985–988. doi: 10.1128/aac.27.6.985

Elimination of multicopy plasmid R6K by bleomycin.

P V Attfield, R J Pinney
PMCID: PMC180205  PMID: 2411218

Abstract

Bleomycin eliminated multicopy plasmid R6K from growing cells of Escherichia coli AB1157 but failed to cure either of the low-copy plasmids R1 or R46. Measurements of R6K-encoded beta-lactamase and of covalently closed plasmid DNA indicated that the drug causes a progressive reduction in plasmid copy number.

Full text

PDF
985

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attfield P. V., Pinney R. J. Plasmid R46-mediated protection against bleomycin is poLA+-dependent. J Gen Microbiol. 1982 Mar;128(3):539–547. doi: 10.1099/00221287-128-3-539. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooper P. K. Characterization of long patch excision repair of DNA in ultraviolet-irradiated Escherichia coli: an inducible function under rec-lex control. Mol Gen Genet. 1982;185(2):189–197. doi: 10.1007/BF00330785. [DOI] [PubMed] [Google Scholar]
  4. Crosa J. H., Luttropp L. K., Falkow S. Mode of replication of the conjugative R-plasmid RSF1040 in Escherichia coli. J Bacteriol. 1976 Apr;126(1):454–466. doi: 10.1128/jb.126.1.454-466.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dale J. W., Smith J. T. The purification and properties of the -lactamase specified by the resistance factor R-1818 in Escherichia coli and Proteus mirabilis. Biochem J. 1971 Jul;123(4):493–500. doi: 10.1042/bj1230493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hahn F. E., Ciak J. Elimination of resistance determinants from R-factor R1 by intercalative compounds. Antimicrob Agents Chemother. 1976 Jan;9(1):77–80. doi: 10.1128/aac.9.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hahn F. E. Experimental elimination of R factors. Antibiot Chemother (1971) 1975;20:196–226. doi: 10.1159/000398468. [DOI] [PubMed] [Google Scholar]
  8. Hanawalt P. C., Cooper P. K., Ganesan A. K., Smith C. A. DNA repair in bacteria and mammalian cells. Annu Rev Biochem. 1979;48:783–836. doi: 10.1146/annurev.bi.48.070179.004031. [DOI] [PubMed] [Google Scholar]
  9. Heffron F., Sublett R., Hedges R. W., Jacob A., Falkow S. Origin of the TEM-beta-lactamase gene found on plasmids. J Bacteriol. 1975 Apr;122(1):250–256. doi: 10.1128/jb.122.1.250-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holmans P. L., Clowes R. C. Transposition of a duplicate antibiotic resistance gene and generation of deletions in plasmid R6K. J Bacteriol. 1979 Feb;137(2):977–989. doi: 10.1128/jb.137.2.977-989.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kontomichalou P., Mitani M., Clowes R. C. Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control. J Bacteriol. 1970 Oct;104(1):34–44. doi: 10.1128/jb.104.1.34-44.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lloyd R. S., Haidle C. W., Hewitt R. R. Bleomycin-induced alkaline-labile damage and direct strand breakage of PM2 DNA. Cancer Res. 1978 Oct;38(10):3191–3196. [PubMed] [Google Scholar]
  13. Lloyd R. S., Haidle C. W., Robberson D. L. Bleomycin fragmentation of duplex DNA occurs as staggered single-strand scissions. Gene. 1979 Nov;7(3-4):303–316. doi: 10.1016/0378-1119(79)90050-7. [DOI] [PubMed] [Google Scholar]
  14. Lloyd R. S., Haidle C. W., Robberson D. L. Site specificity of bleomycin-mediated single-strand scissions and alkali-labile damage in duplex DNA. Gene. 1979 Nov;7(3-4):289–302. doi: 10.1016/0378-1119(79)90049-0. [DOI] [PubMed] [Google Scholar]
  15. Macrina F. L., Weatherly G. G., Curtiss R., 3rd R6K plasmid replication: influence of chromosomal genotype in minicell-producing strains of Escherichia coli K-12. J Bacteriol. 1974 Dec;120(3):1387–1400. doi: 10.1128/jb.120.3.1387-1400.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nordström U. M., Engberg B., Nordström K. Competition for DNA polymerase III between the chromosome and the R-factor R1. Mol Gen Genet. 1974;135(3):185–190. doi: 10.1007/BF00268614. [DOI] [PubMed] [Google Scholar]
  17. Pinney R. J., Bremer K., Smith J. T. R factor elimination by inhibitors of thymidylate synthetase (fluorodeoxyuridine and showdomycin) and the occurrence of single strand breaks in plasmid DNA. Mol Gen Genet. 1974;133(2):163–174. doi: 10.1007/BF00264837. [DOI] [PubMed] [Google Scholar]
  18. Povirk L. F., Hogan M., Dattagupta N. Binding of bleomycin to DNA: intercalation of the bithiazole rings. Biochemistry. 1979 Jan 9;18(1):96–101. doi: 10.1021/bi00568a015. [DOI] [PubMed] [Google Scholar]
  19. Rowbury R. J. The replication of bacterial plasmids. Sci Prog. 1978 Summer;65(258):231–250. [PubMed] [Google Scholar]
  20. Suzuki H., Nagai K., Yamaki H., Tanaka N., Umezawa H. On the mechanism of action of bleomycin: scission of DNA strands in vitro and in vivo. J Antibiot (Tokyo) 1969 Sep;22(9):446–448. doi: 10.7164/antibiotics.22.446. [DOI] [PubMed] [Google Scholar]
  21. Tweats D. J., Smith J. T. Interaction between the plasmid R6K and Escherichia coli with defective DNA polymerase I. Genet Res. 1978 Aug;32(1):25–35. doi: 10.1017/s0016672300018498. [DOI] [PubMed] [Google Scholar]
  22. Veltkamp E., Stuitje A. R. Replication and structure of the bacteriocinogenic plasmids Clo DF13 and CoI E1. Plasmid. 1981 Jan;5(1):76–99. doi: 10.1016/0147-619x(81)90078-0. [DOI] [PubMed] [Google Scholar]
  23. Wechsler J., Kline B. C. Mutation and identification of the F plasmid locus determining resistance to acridine orange curing. Plasmid. 1980 Nov;4(3):276–280. doi: 10.1016/0147-619x(80)90066-9. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES