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. 1985 Aug;28(2):195–199. doi: 10.1128/aac.28.2.195

Protein antigens of encapsulated Klebsiella pneumoniae surface exposed after growth in the presence of subinhibitory concentrations of cephalosporins.

J L Kadurugamuwa, H Anwar, M R Brown, O Zak
PMCID: PMC180218  PMID: 3914857

Abstract

It recently has been reported by us that cephalosporins, at a concentration below that influencing growth rate, reduced the production of enterochelin and capsule formation of iron-depleted Klebsiella pneumoniae. We now report on the antigenicity of the outer membrane components and surface-exposed protein antigens of iron-depleted cells grown in the presence or absence of cephalosporins. All major outer membrane proteins, including iron-regulated membrane proteins, were immunogenic. Encapsulated K. pneumoniae grown in antibiotic-free media had three protein antigens (60, 35.5, and 32.5 kilodaltons) exposed on the surface that were accessible to antibodies. Growth of the same cultures in the presence of subinhibitory concentrations of cephalosporins resulted in the exposure of a greater number of protein antigenic determinants, including iron-regulated membrane proteins, which become readily accessible to antibodies. It was also found that immunoblotting was generally more sensitive than conventional staining of the acrylamide gel with Coomassie blue in the detection of proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anwar H., Brown M. R., Lambert P. A. Effect of nutrient depletion on sensitivity of Pseudomonas cepacia to phagocytosis and serum bactericidal activity at different temperatures. J Gen Microbiol. 1983 Jul;129(7):2021–2027. doi: 10.1099/00221287-129-7-2021. [DOI] [PubMed] [Google Scholar]
  2. Anwar H., Lambert P. A., Brown M. R. Influence of sodium dodecyl sulphate quality on the electrophoretic mobility of the outer membrane proteins of mucoid and non-mucoid Pseudomonas aeruginosa. Biochim Biophys Acta. 1983 Dec 13;761(2):119–125. doi: 10.1016/0304-4165(83)90220-9. [DOI] [PubMed] [Google Scholar]
  3. Bjorn M. J., Iglewski B. H., Ives S. K., Sadoff J. C., Vasil M. L. Effect of iron on yields of exotoxin A in cultures of Pseudomonas aeruginosa PA-103. Infect Immun. 1978 Mar;19(3):785–791. doi: 10.1128/iai.19.3.785-791.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bjorn M. J., Sokol P. A., Iglewski B. H. Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures. J Bacteriol. 1979 Apr;138(1):193–200. doi: 10.1128/jb.138.1.193-200.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown M. R., Melling J. Loss of sensitivity to EDTA by Pseudomonas aeruginosa grown under conditions of Mg-limitation. J Gen Microbiol. 1968 Dec;54(3):439–444. doi: 10.1099/00221287-54-3-439. [DOI] [PubMed] [Google Scholar]
  6. Brown M. R. Nutrient depletion and antibiotic susceptibility. J Antimicrob Chemother. 1977 May;3(3):198–201. doi: 10.1093/jac/3.3.198. [DOI] [PubMed] [Google Scholar]
  7. Costerton J. W., Irvin R. T., Cheng K. J. The role of bacterial surface structures in pathogenesis. Crit Rev Microbiol. 1981;8(4):303–338. doi: 10.3109/10408418109085082. [DOI] [PubMed] [Google Scholar]
  8. Domenico P., Johanson W. G., Jr, Straus D. C. Lobar pneumonia in rats produced by clinical isolates of Klebsiella pneumoniae. Infect Immun. 1982 Jul;37(1):327–335. doi: 10.1128/iai.37.1.327-335.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Filip C., Fletcher G., Wulff J. L., Earhart C. F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973 Sep;115(3):717–722. doi: 10.1128/jb.115.3.717-722.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Finch J. E., Brown M. R. Effect of growth environment on Pseudomonas aeruginosa killing by rabbit polymorphonuclear leudocytes and cationic proteins. Infect Immun. 1978 May;20(2):340–346. doi: 10.1128/iai.20.2.340-346.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Finch J. E., Brown M. R. The influence of nutrient limitation in a chemostat on the sensitivity of Pseudomonas aeruginosa to polymyxin and to EDTA. J Antimicrob Chemother. 1975 Dec;1(4):379–386. doi: 10.1093/jac/1.4.379. [DOI] [PubMed] [Google Scholar]
  12. Finkelstein R. A., Sciortino C. V., McIntosh M. A. Role of iron in microbe-host interactions. Rev Infect Dis. 1983 Sep-Oct;5 (Suppl 4):S759–S777. doi: 10.1093/clinids/5.supplement_4.s759. [DOI] [PubMed] [Google Scholar]
  13. Gemmell C. G., Spear T., Peterson P. K. Morphological changes in bacteroides fragilis and klebsiella pneumoniae attributable to growth in the presence of various antibiotics. Eur J Clin Microbiol. 1983 Jun;2(3):217–221. doi: 10.1007/BF02029520. [DOI] [PubMed] [Google Scholar]
  14. Kadurugamuwa J. L., Anwar H., Brown M. R., Zak O. Effect of subinhibitory concentrations of cephalosporins on surface properties and siderophore production in iron-depleted Klebsiella pneumoniae. Antimicrob Agents Chemother. 1985 Feb;27(2):220–223. doi: 10.1128/aac.27.2.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ombaka E. A., Cozens R. M., Brown M. R. Influence of nutrient limitation of growth on stability and production of virulence factors of mucoid and nonmucoid strains of Pseudomonas aeruginosa. Rev Infect Dis. 1983 Nov-Dec;5 (Suppl 5):S880–S888. doi: 10.1093/clinids/5.supplement_5.s880. [DOI] [PubMed] [Google Scholar]
  16. Shand G. H., Anwar H., Kadurugamuwa J., Brown M. R., Silverman S. H., Melling J. In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions. Infect Immun. 1985 Apr;48(1):35–39. doi: 10.1128/iai.48.1.35-39.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith H. Microbial surfaces in relation to pathogenicity. Bacteriol Rev. 1977 Jun;41(2):475–500. doi: 10.1128/br.41.2.475-500.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Spratt B. G. Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2999–3003. doi: 10.1073/pnas.72.8.2999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Spratt B. G. Properties of the penicillin-binding proteins of Escherichia coli K12,. Eur J Biochem. 1977 Jan;72(2):341–352. doi: 10.1111/j.1432-1033.1977.tb11258.x. [DOI] [PubMed] [Google Scholar]
  20. Swanson J. Surface-exposed protein antigens of the gonococcal outer membrane. Infect Immun. 1981 Dec;34(3):804–816. doi: 10.1128/iai.34.3.804-816.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Troy F. A., Frerman F. E., Heath E. C. The biosynthesis of capsular polysaccharide in Aerobacter aerogenes. J Biol Chem. 1971 Jan 10;246(1):118–133. [PubMed] [Google Scholar]
  23. Waxman D. J., Strominger J. L. Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Annu Rev Biochem. 1983;52:825–869. doi: 10.1146/annurev.bi.52.070183.004141. [DOI] [PubMed] [Google Scholar]
  24. Williams P., Brown M. R., Lambert P. A. Effect of iron deprivation on the production of siderophores and outer membrane proteins in Klebsiella aerogenes. J Gen Microbiol. 1984 Sep;130(9):2357–2365. doi: 10.1099/00221287-130-9-2357. [DOI] [PubMed] [Google Scholar]
  25. Xiu J. H., Magnusson K. E., Stendahl O., Edebo L. Physicochemical surface properties and phagocytosis by polymorphonuclear leucocytes of different serogroups of Salmonella. J Gen Microbiol. 1983 Oct;129(10):3075–3084. doi: 10.1099/00221287-129-10-3075. [DOI] [PubMed] [Google Scholar]
  26. Zak O., Kradolfer F. Effects of subminimal inhibitory concentrations of antibiotics in experimental infections. Rev Infect Dis. 1979 Sep-Oct;1(5):862–879. doi: 10.1093/clinids/1.5.862. [DOI] [PubMed] [Google Scholar]

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