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Epitope mapping of autoantibodies directed against human Fas. Oligopeptides were synthesized on activated membranes using the SPOTs system. The peptides were 12 amino acids in length and had a sequential overlap of nine amino acids. The membranes were blocked, incubated with 1/50 diluted sample sera, then detected using β-galactosidase-conjugated goat anti-human immunoglobulin antibody. Hatched sequences indicate each epitope recognized by the autoantibodies.
