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. 2005 Sep;116(1):21–29. doi: 10.1111/j.1365-2567.2005.02192.x

Figure 6.

Figure 6

(a) Time schedule of siRNA experiments. Details are given in the Materials and methods. (b) The gel image of MP-RT-PCR for the Fas gene in KMS-12PE cells cultured for −24–0 hr with RPMI-1640 medium supplemented with 5% fetal bovine serum with (TF Cont) or without (Cont) transfection control medium or siRNA medium. (c) The relative Fas gene expression level was analysed from the gel-image shown in (b). Although the TF Cont showed a slight reduction, the siRNA sample demonstrated a marked decrease in Fas gene expression. (d) After harvesting RNA samples, each group of cells was resuspended in the appropriate medium containing 5% serum from either HV-6 or SIL-1. After 24 and 48 hr, the WST-1 assay was performed. At both time points, silencing the Fas gene led to a statistically significant recovery of the SIL-1 serum-induced growth inhibition.