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. 1985 Oct;28(4):521–523. doi: 10.1128/aac.28.4.521

Rapid method for determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography.

H Kubo, Y Kobayashi, T Nishikawa
PMCID: PMC180296  PMID: 4073874

Abstract

A rapid, simple, and accurate method for the determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography is described. The serum proteins were precipitated with 3.5% perchloric acid containing sodium octanesulfonate. After centrifugation, a sample of the supernatant was directly injected into the chromatograph. The determination of kanamycin and dibekacin was performed by a combination of reverse-phase, ion-pair chromatography, postcolumn derivatization with o-phthalaldehyde, and fluorescence detection. The correlation coefficients with fluorescence polarization immunoassay were 0.996 for kanamycin and 0.957 for dibekacin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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