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. 1985 Dec;28(6):730–734. doi: 10.1128/aac.28.6.730

Antimicrobial therapy of experimental Legionella micdadei pneumonia in guinea pigs.

A W Pasculle, J N Dowling, F N Frola, D A McDevitt, M A Levi
PMCID: PMC180318  PMID: 3878688

Abstract

Several antimicrobial agents were evaluated for activity against experimental Legionella micdadei pneumonia in guinea pigs. Erythromycin, rifampin, doxycycline, and sulfamethoxazole-trimethoprim produced significant reductions in mortality. Penicillin, cefazolin, cefoxitin, chloramphenicol, and gentamicin were not efficacious even though, at the doses administered, the peak concentrations of these agents in serum substantially exceeded their MICs for the test strain. It is suggested that the poor performance of the latter group of agents resulted from poor penetration into cells in which L. micdadei was multiplying.

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Selected References

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  1. Bacheson M. A., Friedman H. M., Benson C. E. Antimicrobial susceptibility of intracellular Legionella pneumophila. Antimicrob Agents Chemother. 1981 Nov;20(5):691–692. doi: 10.1128/aac.20.5.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dowling J. N., Weyant R. S., Pasculle A. W. Bactericidal activity of antibiotics against Legionella micdadei (Pittsburgh pneumonia agent). Antimicrob Agents Chemother. 1982 Aug;22(2):272–276. doi: 10.1128/aac.22.2.272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dudley M. N., Levitz R. E., Quintiliani R., Hickingbotham J. M., Nightingale C. H. Pharmacokinetics of trimethoprim and sulfamethoxazole in serum and cerebrospinal fluid of adult patients with normal meninges. Antimicrob Agents Chemother. 1984 Dec;26(6):811–814. doi: 10.1128/aac.26.6.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelstein P. H., Calarco K., Yasui V. K. Antimicrobial therapy of experimentally induced Legionnaires' disease in guinea pigs. Am Rev Respir Dis. 1984 Nov;130(5):849–856. doi: 10.1164/arrd.1984.130.5.849. [DOI] [PubMed] [Google Scholar]
  5. Edelstein P. H. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol. 1981 Sep;14(3):298–303. doi: 10.1128/jcm.14.3.298-303.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edelstein P. H., Meyer R. D., Finegold S. M. Laboratory diagnosis of Legionnaires' disease. Am Rev Respir Dis. 1980 Feb;121(2):317–327. doi: 10.1164/arrd.1980.121.2.317. [DOI] [PubMed] [Google Scholar]
  7. Edelstein P. H., Meyer R. D., Finegold S. M. Long-term followup of two patients with pulmonary cavitation caused by Legionella pneumophila. Am Rev Respir Dis. 1981 Jul;124(1):90–93. doi: 10.1164/arrd.1981.124.1.90. [DOI] [PubMed] [Google Scholar]
  8. Farrar W. E., Kent T. H., Elliott V. B. Lethal gram-negative bacterial superinfection in Guinea pigs given bacitracin. J Bacteriol. 1966 Aug;92(2):496–501. doi: 10.1128/jb.92.2.496-501.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fraser D. W., Wachsmuth I., Bopp C., Feeley J. C., Tsai T. F. Antibiotic treatment of guinea-pigs infected with agent of Legionnaires' disease. Lancet. 1978 Jan 28;1(8057):175–178. doi: 10.1016/s0140-6736(78)90611-6. [DOI] [PubMed] [Google Scholar]
  10. Gibson D. H., Fitzgeorge R. B., Baskerville A. Antibiotic therapy of experimental airborne Legionnaires' disease. J Infect. 1983 Nov;7(3):210–217. doi: 10.1016/s0163-4453(83)97034-2. [DOI] [PubMed] [Google Scholar]
  11. Gibson D. H., Fitzgeorge R. B. Persistence in serum and lungs of guinea pigs of erythromycin, gentamicin, chloramphenicol and rifampicin and their in-vitro activities against Legionella pneumophila. J Antimicrob Chemother. 1983 Sep;12(3):235–244. doi: 10.1093/jac/12.3.235. [DOI] [PubMed] [Google Scholar]
  12. Hand W. L., Corwin R. W., Steinberg T. H., Grossman G. D. Uptake of antibiotics by human alveolar macrophages. Am Rev Respir Dis. 1984 Jun;129(6):933–937. doi: 10.1164/arrd.1984.129.6.933. [DOI] [PubMed] [Google Scholar]
  13. Horwitz M. A., Silverstein S. C. Intracellular multiplication of Legionnaires' disease bacteria (Legionella pneumophila) in human monocytes is reversibly inhibited by erythromycin and rifampin. J Clin Invest. 1983 Jan;71(1):15–26. doi: 10.1172/JCI110744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Muder R. R., Yu V. L., Zuravleff J. J. Pneumonia due to the Pittsburgh pneumonia agent: new clinical perspective with a review of the literature. Medicine (Baltimore) 1983 Mar;62(2):120–128. doi: 10.1097/00005792-198303000-00005. [DOI] [PubMed] [Google Scholar]
  15. Myerowitz R. L., Pasculle A. W., Dowling J. N., Pazin G. J., Sr, Puerzer M., Yee R. B., Rinaldo C. R., Jr, Hakala T. R. Opportunistic lung infection due to "Pittsburgh Pneumonia Agent". N Engl J Med. 1979 Nov 1;301(18):953–958. doi: 10.1056/NEJM197911013011801. [DOI] [PubMed] [Google Scholar]
  16. Pasculle A. W., Dowling J. N., Weyant R. S., Sniffen J. M., Cordes L. G., Gorman G. M., Feeley J. C. Susceptibility of Pittsburgh pneumonia agent (Legionella micdadei) and other newly recognized members of the genus Legionella to nineteen antimicrobial agents. Antimicrob Agents Chemother. 1981 Dec;20(6):793–799. doi: 10.1128/aac.20.6.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pasculle A. W., Feeley J. C., Gibson R. J., Cordes L. G., Myerowitz R. L., Patton C. M., Gorman G. W., Carmack C. L., Ezzell J. W., Dowling J. N. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis. 1980 Jun;141(6):727–732. doi: 10.1093/infdis/141.6.727. [DOI] [PubMed] [Google Scholar]
  18. Pasculle A. W., Myerowitz R. L., Rinaldo C. R., Jr New bacterial agent of pneumonia isolated from renal-transplant recipients. Lancet. 1979 Jul 14;2(8133):58–61. doi: 10.1016/s0140-6736(79)90117-x. [DOI] [PubMed] [Google Scholar]
  19. Prokesch R. C., Hand W. L. Antibiotic entry into human polymorphonuclear leukocytes. Antimicrob Agents Chemother. 1982 Mar;21(3):373–380. doi: 10.1128/aac.21.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rogers B. H., Donowitz G. R., Walker G. K., Harding S. A., Sande M. A. Opportunistic pneumonia: a clinicopathological study of five cases caused by an unidentified acid-fast bacterium. N Engl J Med. 1979 Nov 1;301(18):959–961. doi: 10.1056/NEJM197911013011802. [DOI] [PubMed] [Google Scholar]
  21. Rudin J. E., Wing E. J. A comparative study of Legionella micdadei and other nosocomial acquired pneumonia. Chest. 1984 Nov;86(5):675–680. doi: 10.1378/chest.86.5.675. [DOI] [PubMed] [Google Scholar]
  22. Sabath L. D., Casey J. I., Ruch P. A., Stumpf L. L., Finland M. Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma. J Lab Clin Med. 1971 Sep;78(3):457–463. [PubMed] [Google Scholar]
  23. Seger R. A., Baumgartner S., Tiefenauer L. X., Gmünder F. K. Chronic granulomatous disease: effect of sulfamethoxazole/trimethoprim on neutrophil microbicidal function. Helv Paediatr Acta. 1981;36(6):579–588. [PubMed] [Google Scholar]
  24. Steigbigel N. H., Reed C. W., Finland M. Absorption and excretion of five tetracycline analogues in normal young men. Am J Med Sci. 1968 May;255:296–312. doi: 10.1097/00000441-196805000-00005. [DOI] [PubMed] [Google Scholar]
  25. Toro J., Sawatari K., Kanda T., Saito A., Hara K. New beta-lactamase-resistant cephem treatment of guinea pigs infected with Legionella pneumophila. Microbiol Immunol. 1983;27(8):649–654. doi: 10.1111/j.1348-0421.1983.tb00627.x. [DOI] [PubMed] [Google Scholar]
  26. Vree T. B., Hekster Y. A., Baars A. M., Damsma J. E., Kleijin E. V. Determination of trimethoprim and sulfamethoxazole (co-trimoxazole) in body fluids of man by means of high-performance liquid chromatography. J Chromatogr. 1978 Jul 1;146(1):103–112. doi: 10.1016/s0378-4347(00)81294-3. [DOI] [PubMed] [Google Scholar]
  27. Wing E. J., Schafer F. J., Pasculle A. W. Successful treatment of Legionella micdadei (Pittsburgh pneumonia agent) pneumonia with erythromycin. Am J Med. 1981 Nov;71(5):836–840. doi: 10.1016/0002-9343(81)90376-4. [DOI] [PubMed] [Google Scholar]

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