Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1985 Dec;28(6):773–777. doi: 10.1128/aac.28.6.773

Cephamycin inactivation due to enzymatic hydrolysis by beta-lactamase from Bacteroides fragilis.

A Yotsuji, S Minami, H Kakizawa, T Yasuda, A Takai, I Saikawa, M Inoue, S Mitsuhashi
PMCID: PMC180326  PMID: 3878690

Abstract

The susceptibility of 53 clinical isolates of Bacteroides fragilis to cephamycins was examined. Judging from the MICs for 50% of the strains tested, moxalactam was the most active, however, judging from the MICs for 90% of the strains tested, cefbuperazone was more effective than moxalactam. A correlation was observed between in vitro activity of benzylpenicillin and cephaloridine and beta-lactamase production. Inactivation due to enzymatic hydrolysis of cephamycins over a short time was not observed; however, inactivation was detected by a double disk diffusion test, and moxalactam was most easily inactivated. We conclude that inactivation due to enzymatic hydrolysis of cephamycins over a long time may play an important role in resistance to some cephamycins in strains of B. fragilis.

Full text

PDF
773

Images in this article

774Image
on p.774

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. D., Sykes R. B. Characterisation of a -lactamase obtained from a strain of Bacteroides fragilis resistant to -lactam antibiotics. J Med Microbiol. 1973 May;6(2):201–206. doi: 10.1099/00222615-6-2-201. [DOI] [PubMed] [Google Scholar]
  2. Dornbusch K., Nord C. E., Olsson B. Antibiotic susceptibility testing of anaerobic bacteria by the standardized disc diffusion method with special reference to bacteroides fragilis. Scand J Infect Dis. 1975;7(1):59–66. doi: 10.3109/inf.1975.7.issue-1.11. [DOI] [PubMed] [Google Scholar]
  3. Keim G. R., Sibley P. L., Hines F. A., Miller M. M., Peterson A. E., Yoon Y. H. Parenteral toxicological profile of the monocyclic beta-lactam antibiotic SQ 26,776 in mice, rats and dogs. J Antimicrob Chemother. 1981 Dec;8 (Suppl E):141–146. doi: 10.1093/jac/8.suppl_e.141. [DOI] [PubMed] [Google Scholar]
  4. Masuda G., Tomioka S., Hasegawa M. Detection of beta-lactamase production by gram-negative bacteria. J Antibiot (Tokyo) 1976 Jun;29(6):662–664. doi: 10.7164/antibiotics.29.662. [DOI] [PubMed] [Google Scholar]
  5. Minami S., Matsubara N., Yotsuji A., Araki H., Watanabe Y., Yasuda T., Saikawa I., Mitsuhashi S. Inactivation of cephamycins by various beta-lactamases from gram-negative bacteria. J Antibiot (Tokyo) 1984 May;37(5):577–587. doi: 10.7164/antibiotics.37.577. [DOI] [PubMed] [Google Scholar]
  6. Minami S., Matsubara N., Yotsuji A., Watanabe Y., Yasuda T., Saikawa I., Mitsuhashi S. Antibacterial activity of cefoperazone alone and in combination against cephalosporinase-producing Enterobacter cloacae. Antimicrob Agents Chemother. 1983 Jul;24(1):123–125. doi: 10.1128/aac.24.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Minami S., Yotsuji A., Inoue M., Mitsuhashi S. Induction of beta-lactamase by various beta-lactam antibiotics in Enterobacter cloacae. Antimicrob Agents Chemother. 1980 Sep;18(3):382–385. doi: 10.1128/aac.18.3.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Olson B., Weinstein R. A., Nathan C., Kabins S. A. Broad-spectrum beta-lactam resistance in Enterobacter: emergence during treatment and mechanisms of resistance. J Antimicrob Chemother. 1983 Apr;11(4):299–310. doi: 10.1093/jac/11.4.299. [DOI] [PubMed] [Google Scholar]
  9. Olsson B., Nord C. E., Wadström T. Formation of beta-lactamase in Bacteroides fragilis: cell-bound and extracellular activity. Antimicrob Agents Chemother. 1976 May;9(5):727–735. doi: 10.1128/aac.9.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sato K., Inoue M., Mitsuhashi S. Activity of beta-lactamase produced by Bacteroides fragilis against newly introduced cephalosporins. Antimicrob Agents Chemother. 1980 Apr;17(4):736–737. doi: 10.1128/aac.17.4.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sutter V. L., Finegold S. M. Susceptibility of anaerobic bacteria to 23 antimicrobial agents. Antimicrob Agents Chemother. 1976 Oct;10(4):736–752. doi: 10.1128/aac.10.4.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tai M., Fukuoka Y., Yotsuji A., Kumano K., Takahata M., Mikami H., Yasuda T., Saikawa I., Mitsuhashi S. In vitro and in vivo antibacterial activity of T-1982, a new semisynthetic cephamycin antibiotic. Antimicrob Agents Chemother. 1982 Nov;22(5):728–734. doi: 10.1128/aac.22.5.728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Then R. L., Angehrn P. Trapping of nonhydrolyzable cephalosporins by cephalosporinases in Enterobacter cloacae and Pseudomonas aeruginosa as a possible resistance mechanism. Antimicrob Agents Chemother. 1982 May;21(5):711–717. doi: 10.1128/aac.21.5.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Toda M., Ikeuchi T., Tajima M., Inoue M., Mitsuhashi S. Comparative inhibition of beta-lactamases by cephamycin antibiotics. J Antibiot (Tokyo) 1981 Jan;34(1):114–117. doi: 10.7164/antibiotics.34.114. [DOI] [PubMed] [Google Scholar]
  15. Vu H., Nikaido H. Role of beta-lactam hydrolysis in the mechanism of resistance of a beta-lactamase-constitutive Enterobacter cloacae strain to expanded-spectrum beta-lactams. Antimicrob Agents Chemother. 1985 Mar;27(3):393–398. doi: 10.1128/aac.27.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yoshida T., Matsuura S., Mayama M., Kameda Y., Kuwahara S. Moxalactam (6059-S), a novel 1-oxa-beta-lactam with an expanded antibacterial spectrum: laboratory evaluation. Antimicrob Agents Chemother. 1980 Mar;17(3):302–312. doi: 10.1128/aac.17.3.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yotsuji A., Minami S., Araki Y., Inoue M., Mitsuhashi S. Inducer activity of beta-lactam antibiotics for the beta-lactamases of Proteus rettgeri and Proteus vulgaris. J Antibiot (Tokyo) 1982 Nov;35(11):1590–1593. doi: 10.7164/antibiotics.35.1590. [DOI] [PubMed] [Google Scholar]
  18. Yotsuji A., Minami S., Inoue M., Mitsuhashi S. Properties of novel beta-lactamase produced by Bacteroides fragilis. Antimicrob Agents Chemother. 1983 Dec;24(6):925–929. doi: 10.1128/aac.24.6.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yotsuji A., Minami S., Okamoto S., Yasuda T., Takai A., Saikawa I., Inoue M., Mitsuhashi S. Immunological properties of a novel beta-lactamase produced by Bacteroides fragilis. J Antibiot (Tokyo) 1984 Nov;37(11):1483–1485. doi: 10.7164/antibiotics.37.1483. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES