Abstract
The addition of NaCl to cation-supplemented Mueller-Hinton broth increased the MICs of gentamicin, amikacin, and netilmicin for coagulase-positive and -negative staphylococci in a concentration-dependent manner. At 2% NaCl, geometric mean MICs were elevated 14.1- to 25.6-fold. Mueller-Hinton broth containing added NaCl should not be used for testing the susceptibility of staphylococci to aminoglycosides.
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Selected References
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- BARBER M. Methicillin-resistant staphylococci. J Clin Pathol. 1961 Jul;14:385–393. doi: 10.1136/jcp.14.4.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benveniste R., Davies J. Mechanisms of antibiotic resistance in bacteria. Annu Rev Biochem. 1973;42:471–506. doi: 10.1146/annurev.bi.42.070173.002351. [DOI] [PubMed] [Google Scholar]
- Edberg S. C., Bottenbley C. J., Gam K. Use of sodium polyanethol sulfonate to selectively inhibit aminoglycoside and polymyxin antibiotics in a rapid blood level antibiotic assay. Antimicrob Agents Chemother. 1976 Mar;9(3):414–417. doi: 10.1128/aac.9.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hewitt J. H., Coe A. W., Parker M. T. The detection of methicillin resistance in Staphylococcus aureus. J Med Microbiol. 1969 Nov 4;2(4):443–456. doi: 10.1099/00222615-2-4-443. [DOI] [PubMed] [Google Scholar]
- Mates S. M., Eisenberg E. S., Mandel L. J., Patel L., Kaback H. R., Miller M. H. Membrane potential and gentamicin uptake in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6693–6697. doi: 10.1073/pnas.79.21.6693. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mates S. M., Patel L., Kaback H. R., Miller M. H. Membrane potential in anaerobically growing Staphylococcus aureus and its relationship to gentamicin uptake. Antimicrob Agents Chemother. 1983 Apr;23(4):526–530. doi: 10.1128/aac.23.4.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reller L. B., Schoenknecht F. D., Kenny M. A., Sherris J. C. Antibiotic susceptibility testing of Pseudomonas aeruginosa: selection of a control strain and criteria for magnesium and calcium content in media. J Infect Dis. 1974 Nov;130(5):454–463. doi: 10.1093/infdis/130.5.454. [DOI] [PubMed] [Google Scholar]
- Thornsberry C., McDougal L. K. Successful use of broth microdilution in susceptibility tests for methicillin-resistant (heteroresistant) staphylococci. J Clin Microbiol. 1983 Nov;18(5):1084–1091. doi: 10.1128/jcm.18.5.1084-1091.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]