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. 1986 Dec;30(6):883–887. doi: 10.1128/aac.30.6.883

Microplate phosphocellulose binding assay for aminoglycoside-modifying enzymes.

R C Cooksey, B G Metchock, C Thornsberry
PMCID: PMC180612  PMID: 3028252

Abstract

We modified the phosphocellulose binding assay for aminoglycoside-modifying enzymes (AMEs) by use of microdilution plates and a multichannel micropipette. Batteries of aminoglycoside substrates for screening organisms for the presence of AMEs as well as for subclassifying enzymes were prepared and stored in microdilution plates. When tested in parallel with the conventional tube reaction assay, the microplate assay yielded comparable radioactive counts and therefore equally correct identifications of AMEs in 32 isolates representing nine bacterial species. Other modifications, such as multichannel dispensing of crude enzyme preparations and radioisotopic precursors, provided a more rapid, convenient, and less expensive means of examining large collections of organisms for AMEs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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