Gagliardi M.C., Finocchi A., Orlandi P., Cursi L., Cancrini C., Moschese V., Miyawaki T. & Rossi P. Bruton's tyrosine kinase defect in dendritic cells from X-linked agammaglobulinaemia patients does not influence their differentiation, maturation and antigen-presenting cell function. Clin Exp Immunol 2003; 131:115–122.
In this article, page 121, 2nd column, line 8, the sentence was incorrectly printed as “In our DC maturation we induced DC maturation … ”. However, it should have read “In our experiments we induced DC maturation … ”.
Figure 3 was printed in a format that was different to that required by the author: Fig. 3 is reprinted below in the format originally provided by the author.
Fig. 3.
Stimulation of naïve T cells by XLA DC. Immature DC (○) were left untreated or stimulated with LPS for 24 h (▪). A mixed leucocyte reaction (MLR) was then set up where irradiated DC from four XLA patients (A–D) and two healthy donors were cultured at different cell numbers with 1×105 unfractionated cord blood cells. The proliferative response was measured after 5 days by thymidine incorporation. The background proliferation of cord blood cells alone was <100 cpm. The data shown are from one representative experiment out of two performed.
In Fig. 4, panels (a) and (b) were incorrectly labelled in the figure as “Healthy donor”, when they referred to XLA patients. The corrected Fig. 4 is printed below.
Fig. 4.
Intracellular staining for IL-4 and IFN-γ produced by cord blood T cells. T cells (90–95% CD4+, data not shown) expanded from the MLR assays shown in Fig. 3 (A–D, XLA patients; and two healthy donors) were stimulated with PMA and ionomycin for 4 h. Cells were fixed, permeabilized and stained with FITC-conjugated anti-IFN-γ and PE-conjugated anti-IL-4 mAbs. The numbers indicate the percentage of cells in each quadrant.