Abstract
After inducing mutagenesis with nitrosoguanidine, we used vancomycin enrichment in isolating auxotrophic mutants of Mycobacterium smegmatis. Compared with cycloserine and penicillin G, which are also cell wall inhibitors, vancomycin, with 24 h of exposure, produced less cell lysis and a greater reduction in viable cell numbers. With vancomycin enrichment, 13 specific auxotrophs were isolated after nitrosoguanidine-induced mutation, whereas only two mutants were isolated without enrichment.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Grange J. M. The genetics of mycobacteria and mycobacteriophages - a review. Tubercle. 1975 Sep;56(3):227–238. doi: 10.1016/0041-3879(75)90056-2. [DOI] [PubMed] [Google Scholar]
- Guerola N., Ingraham J. L., Cerdá-Olmedo E. Induction of closely linked multiple mutations by nitrosoguanidine. Nat New Biol. 1971 Mar 24;230(12):122–125. doi: 10.1038/newbio230122a0. [DOI] [PubMed] [Google Scholar]
- Holland K. T., Ratledge C. A procedure for selecting and isolating specific auxotrophic mutants of Mycobacterium smegmatis. J Gen Microbiol. 1971 Apr;66(1):115–118. doi: 10.1099/00221287-66-1-115. [DOI] [PubMed] [Google Scholar]
- Horne D., Tomasz A. Tolerant response of Streptococcus sanguis to beta-lactams and other cell wall inhibitors. Antimicrob Agents Chemother. 1977 May;11(5):888–896. doi: 10.1128/aac.11.5.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JORDAN D. C. Effect of vancomycin on the synthesis of the cell wall mucopeptide of Staphylococcus aureus. Biochem Biophys Res Commun. 1961 Nov 20;6:167–170. doi: 10.1016/0006-291x(61)90122-x. [DOI] [PubMed] [Google Scholar]
- REYNOLDS P. E. Studies on the mode of action of vancomycin. Biochim Biophys Acta. 1961 Sep 16;52:403–405. doi: 10.1016/0006-3002(61)90698-9. [DOI] [PubMed] [Google Scholar]
- Slosárek M., Konícková-Radochová M., Konícek J. Genetic transfers in Mycobacteria. Folia Microbiol (Praha) 1978;23(2):140–151. doi: 10.1007/BF02915315. [DOI] [PubMed] [Google Scholar]