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. 1981 May;19(5):934–936. doi: 10.1128/aac.19.5.934

Procedure for Assay of Tobramycin in Serum Containing Moxalactam

David F Mahoney 1, T F Butler 1, J R Turner 1
PMCID: PMC181547  PMID: 6457559

Abstract

Moxalactam was examined as a substrate for 11 β-lactamases. Hydrolysis was shown only with the β-lactamase from Bacillus cereus, a nonpathogenic bacterium. Tobramycin was assayed in tobramycin-moxalactam mixtures by using this β-lactamase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Datta N., Richmond M. H. The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli. Biochem J. 1966 Jan;98(1):204–209. doi: 10.1042/bj0980204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davies R. B., Abraham E. P. Separation, purification and properties of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9. Biochem J. 1974 Oct;143(1):115–127. doi: 10.1042/bj1430115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lamb J. W., Mann J. M., Simmons R. J. Factors influencing the microbiological assay of tobramycin. Antimicrob Agents Chemother. 1972 Apr;1(4):323–328. doi: 10.1128/aac.1.4.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mahoney D. F., Koppel G. A., Turner J. R. Substrate inhibition of beta-lactamases, a method for predicting enzymatic stability of cephalosporins. Antimicrob Agents Chemother. 1976 Sep;10(3):470–475. doi: 10.1128/aac.10.3.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. O'Callaghan C. H., Muggleton P. W., Ross G. W. Effects of beta-lactamase from gram-negative organisms on cephalosporins and penicillins. Antimicrob Agents Chemother (Bethesda) 1968;8:57–63. [PubMed] [Google Scholar]
  7. Richmond M. H. Beta-lactamase (Staphylococcus aureus). Methods Enzymol. 1975;43:664–672. doi: 10.1016/0076-6879(75)43131-7. [DOI] [PubMed] [Google Scholar]
  8. Richmond M. H. The beta-lactamase stability of a novel beta-lactam antibiotic containing a 7 alpha-methoxyoxacephem nucleus. J Antimicrob Chemother. 1980 Jul;6(4):445–453. doi: 10.1093/jac/6.4.445. [DOI] [PubMed] [Google Scholar]
  9. Ross G. W., Boulton M. G. Purification of beta-lactamases on QAE-sephadex. Biochim Biophys Acta. 1973 Jun 6;309(2):430–439. doi: 10.1016/0005-2744(73)90041-7. [DOI] [PubMed] [Google Scholar]
  10. Sato K., Inoue M., Mitsuhashi S. Activity of beta-lactamase produced by Bacteroides fragilis against newly introduced cephalosporins. Antimicrob Agents Chemother. 1980 Apr;17(4):736–737. doi: 10.1128/aac.17.4.736. [DOI] [PMC free article] [PubMed] [Google Scholar]

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