Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1981 Jun;19(6):958–964. doi: 10.1128/aac.19.6.958

Aminoglycoside-resistant mutants of Pseudomonas aeruginosa deficient in cytochrome d, nitrite reductase, and aerobic transport.

L E Bryan, S Kwan
PMCID: PMC181592  PMID: 6791588

Abstract

Two gentamicin-resistant mutants of Pseudomonas aeruginosa PAO 503 were selected after ethyl methane sulfonate mutagenesis. Mutant PAO 2403 had significantly increased resistance to aminoglycoside but not to other antibiotics. Mutant PAO 2402 showed a similar spectrum of resistance but of lower magnitude. Both mutants showed no detectable cytochrome d and had a high frequency of reversion to a fully wild-type phenotype. PAO 2403 had a marked decrease and PAO 2402 had a moderate decrease in nitrite reductase activity. Both mutants had reduced uptake of gentamicin and dihydrostreptomycin. Mutant PAO 2403 showed a general decrease in transport rate of cationic compounds, whereas mutant PAO 2402 had only deficient glucose transport. Both mutants showed enhanced rates of glutamine transport and no change in glutamic acid transport. Other components of electron transport and oxidative phosphorylation were normal. These mutants involve ferrocytochrome C551 oxidoreductase formed only on anaerobic growth but illustrate transport defects in aerobically grown cells.

Full text

PDF
958

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Bryan L. E., Nicas T., Holloway B. W., Crowther C. Aminoglycoside-resistant mutation of Pseudomonas aeruginosa defective in cytochrome c552 and nitrate reductase. Antimicrob Agents Chemother. 1980 Jan;17(1):71–79. doi: 10.1128/aac.17.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bryan L. E., Van Den Elzen H. M. Effects of membrane-energy mutations and cations on streptomycin and gentamicin accumulation by bacteria: a model for entry of streptomycin and gentamicin in susceptible and resistant bacteria. Antimicrob Agents Chemother. 1977 Aug;12(2):163–177. doi: 10.1128/aac.12.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bryan L. E., Van Den Elzen H. M. Gentamicin accumulation by sensitive strains of Escherichia coli and Pseudomonas aeruginosa. J Antibiot (Tokyo) 1975 Sep;28(9):696–703. doi: 10.7164/antibiotics.28.696. [DOI] [PubMed] [Google Scholar]
  5. Bryan L. E., Van den Elzen H. M. Streptomycin accumulation in susceptible and resistant strains of Escherichia coli and Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1976 Jun;9(6):928–938. doi: 10.1128/aac.9.6.928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dickie P., Bryan L. E., Pickard M. A. Effect of enzymatic adenylylation on dihydrostreptomycin accumulation in Escherichia coli carrying an R-factor: model explaining aminoglycoside resistance by inactivating mechanisms. Antimicrob Agents Chemother. 1978 Oct;14(4):569–580. doi: 10.1128/aac.14.4.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eagon R. G., Phibbs P. V., Jr Kinetics of transport of glucose, fructose, and mannitol by Pseudomonas aeruginosa. Can J Biochem. 1971 Sep;49(9):1031–1041. doi: 10.1139/o71-151. [DOI] [PubMed] [Google Scholar]
  8. Mills B. J., Holloway B. W. Mutants of Pseudomonas aeruginosa that show specific hypersensitivity to aminoglycosides. Antimicrob Agents Chemother. 1976 Sep;10(3):411–416. doi: 10.1128/aac.10.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Niven D. F., Hamilton W. A. Mechanisms of energy coupling to the transport of amino acids by Staphylococcus aureus. Eur J Biochem. 1974 May 15;44(2):517–522. doi: 10.1111/j.1432-1033.1974.tb03510.x. [DOI] [PubMed] [Google Scholar]
  10. Wharton D. C., Gudat J. C., Gibson Q. H. Cytochrome oxidase from Pseudomonas aeruginosa. I. Reaction with copper protein. Biochim Biophys Acta. 1973 Apr 5;292(3):611–620. doi: 10.1016/0005-2728(73)90009-1. [DOI] [PubMed] [Google Scholar]
  11. YAMANAKA T., OTA A., OKUNUKI K. Nitrite reductase activity of Pseudomonas cytochrome oxidase. Biochim Biophys Acta. 1960 Nov 4;44:397–398. doi: 10.1016/0006-3002(60)91593-6. [DOI] [PubMed] [Google Scholar]
  12. van Hartingsveldt J., Stouthamer A. H. Mapping and characerization of mutants of Pseudomonas aeruginosa affected in nitrate respiration in aerobic or anaerobic growth. J Gen Microbiol. 1973 Jan;74(1):97–106. doi: 10.1099/00221287-74-1-97. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES